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Planta

, Volume 198, Issue 3, pp 433–439 | Cite as

Induction of embryogenesis with colchicine instead of heat in microspores ofBrassica napus L. cv. Topas

  • Ji-Ping Zhao
  • Daina H. Simmonds
  • William Newcomb
Article

Abstract

Prior to this report, heat treatment (32.5°C, 24 h) was the method used to induce embryogenesis fromBrassica napus microspores. Continuous culture at 25°C results in pollen development. This study shows that colchicine alone, at the non-inductive temperature of 25°C, can induce embryogenesis, thus demonstrating that heat shock is not required for embryogenic induction inB. napus cv. Topas. Embryogenic frequencies of over 15% were obtained by culturing isolated microspores with 25 μM colchicine for 42 h at 25°C. The microspore developmental stages responsive to colchicine were unicellular vacuolate and late unicellular, somewhat earlier stages than the population responsive to heat induction. Other groups have reported that heat-shock proteins are essential to the induction of embryogenesis. The present study offers a method of embryogenic induction without the use of heat which will allow discrimination between the factors associated with response to heat shock and those involved with changing cell development.

Key words

Brassica napus Colchicine Heat shock Microspore embryogenesis Microtubules 

Abbreviations

LU

Late-unicellular

PPB

Preprophase band

UV

unicellular-vacuolate

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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Ji-Ping Zhao
    • 1
  • Daina H. Simmonds
    • 2
  • William Newcomb
    • 1
  1. 1.Department of BiologyQueen's UniversityKingstonCanada
  2. 2.Plant Research Centre, Agriculture Canada, CEF#21OttawaCanada

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