, Volume 198, Issue 3, pp 385–396 | Cite as

Isolation of high-chlorophyll-fluorescence mutants ofArabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and Northern hybridisation

  • Jörg Meurer
  • Karin Meierhoff
  • Peter WesthoffEmail author


Thirty-four recessive photosynthetic mutants of the high-chlorophyll-fluorescence (hcf) phenotype have been isolated by screening 7700 M2 progenies of ethyl methane sulfonate-treated seeds ofArabidopsis thaliana. Most of the mutants isolated were found to be seedlinglethal, but could be grown on sucrose-supplemented media. Chlorophyll (Chl) fluorescence induction, absorption changes in the reaction-centre chlorophyll of PS I (P700) at 830 nm and Chla/Chlb ratios were recorded in order to probe the photosynthetic functions and to define the mutational lesion. These studies were complemented by immunoblot and Northern analyses which finally led to the classification of the mutants into six different groups. Four classes of mutants were affected in PS I, PS II (two different classes) or the intersystem electron-transport chain, respectively. A fifth mutant class was of pleiotropic nature and the sixth class comprised a Chlb-deficient mutant. Several of the mutants showed severe deficiencies in the levels of subunits of PS I, PS II or the cytochromeb6/f complex. Thus the mutational lesions could be located precisely. Only one mutant was defective in the transcript patterns of some plastid-encoded photosynthesis genes. Hence most of the mutants isolated appear to be affected in translational and post-translational regulatory processes of thylakoid membrane biogenesis or in structural genes encoding constituent subunits of the thylakoid protein complexes.

Key words

Arabidopsis High-chlorophyll-fluorescence mutants Photosynthesis 

Abbreviations and Symbols




in-vivo absorbance change of P700 at 830 nm measured immediately after interruption of the actinic white light which had been given for four minutes


in-vivo absorbance change of P700 at 830 nm induced by background far-red light at 720 nm


ethyl methane sulfonate

Fo and Fm

minimal and maximal Chla fluorescence of dark-adapted leaves

F′o, F′m and Fs

minimal, maximal and steady-state Chla fluorescence of light-adapted leaves


variable fluorescence


quantum yield of PS I


quantum yield of PS II


high chlorophyll fluorescence


reaction-centre chlorophyll of PS I


non-photochemical quenching


photochemical quenching


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Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  1. 1.Institut für Entwicklungs- und Molekularbiologie der PflanzenHeinrich-Heine-UniversitätDüsseldorfGermany

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