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Planta

, Volume 198, Issue 3, pp 365–370 | Cite as

Glutathione synthesis in maize genotypes with different sensitivities to chilling

  • Gábor Kocsy
  • Monika Brunner
  • Adrian Rüegsegger
  • Peter Stamp
  • Christian BrunoldEmail author
Article

Abstract

The effect of chilling on enzymes, substrates and products of sulfate reduction, gultathione synthesis and metabolism was studied in shoots and roots of maize (Zea mays L.) genotypes with different chilling sensitivity. At full expansion of the second leaf, chilling at 12 °C inhibited dry weight increase in shoots and roots compared to controls at 25 °C and induced an increase in adenosine 5′-phosphosulfate sulfotransferase and γ-glutamylcysteine synthetase (EC 6.3.2.2) activity in the second leaf of all genotypes tested. Glutathione synthetase (EC 6.3.2.3) activity was about one order of magnitude higher than γ-glutamylcysteine synthetase activity, but remained unchanged during chilling except for one genotype. During chilling, cysteine and glutathione content of second leaves increased to significantly higher levels in the two most chilling-tolerant genotypes. Comparing the most tolerant and most sensitive genotype showed that chilling induced a greater incorporation of35S from [35S]sulfate into cysteine and glutathione in the chilling-tolerant than in the sensitive genotype. Chilling decreased the amount of35S-label incorporated into proteins in shoots of both genotypes, but had no effect on this incorporation in the roots. Glutathione reductase (EC 1.6.4.2) and nitrate reductase (EC 1.6.6.1) activity were constitutively higher in the chilling-tolerant genotypes, but showed no changes in most examined genotypes during 3 d at 12 °C. Our results indicate that in maize glutathione is involved in protection against chilling damage.

Key words

Chilling Glutathione synthesis Sulfate reductionZea 

Abbreviations

APSSTase

adenosine 5′-phosphosulfate sulfotransferase

γEC

γ-glutamylcysteine

GR

glutathione reductase

OSH

glutathione

NR

nitrate reductase

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References

  1. Anderson JV, Chevone BI, Hess JL (1992) Seasonal variation in the antioxidant system of eastern white pine needles. Plant Physiol 98: 501–508Google Scholar
  2. Asada K (1994) Production and action of active oxygen species in photosynthetic tissues. In: Foyer CH, Mullineaux PM (eds) Causes of photooxidative stress and amelioration of defense systems in plants. CRC Press, Boca Raton, pp 77–104Google Scholar
  3. Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye-binding. Anal Biochem 72: 248–254Google Scholar
  4. Brunold C (1990) Reduction of sulfate to sulfide. In: Rennenberg H, Brunold C, de Kok LJ, Stulen I (eds) Sulfur nutrition and sulfur assimilation in higher plants. SPB Academic Publishing bv, The Hague, pp 13–31Google Scholar
  5. Brunold C (1993) Regulatory interactions between sulfate and nitrate reduction. In: de Kok LJ, Stulen I, Rennenberg H, Brunold C, Rauser WE (eds) Sulfur nutrition and assimilation in higher plants. SPB Academic Publishing bv, The Hague, pp 61–75Google Scholar
  6. Brunold C, Suter M (1984) Regulation of sulfate assimilation by nitrogen nutrition in the duckweedLemna minor L. Plant Physiol 76: 579–583Google Scholar
  7. Brunold C, Suter M (1990) Adenosine 5′-phosphosulfate sulfotransferase. In: Lea P (ed) Methods in plant biochemistry. Academic Press, London, pp 339–343Google Scholar
  8. De Kok LJ, Oosterhuis FA (1983) Effects of frost-hardening and salinity on glutathione and sulfhydryl levels and on glutathione reductase activity in spinach leaves. Physiol Plant 58: 47–51Google Scholar
  9. Edwards EA, Enard C, Creissen GP, Mullineaux PM (1994) Synthesis and properties of glutathione reductase in stressed peas. Planta 192: 137–143Google Scholar
  10. Esterbauer H, Grill D (1978) Seasonal variation of glutathione and glutathione reductase in needles ofPicea abies. Plant Physiol 61: 119–121Google Scholar
  11. Foyer CH, Halliwell B (1976) The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. Planta 133: 21–25Google Scholar
  12. Foyer CH, Lelandais M, Kunert KJ (1994) Photooxidative stress in plants. Physiol Plant 92: 696–717Google Scholar
  13. Guy CL, Carter JV (1984) Characterization of partially purified glutathione reductase from cold hardened and nonhardened spinach leaf tissue. Cryobiology 21: 454–464Google Scholar
  14. Guy CL, Carter JV, Yelenosky G, Guy CT (1984) Changes in glutathione content during cold acclimation inCornus sericea andCitrus sinensis. Cryobiology 21: 443–453Google Scholar
  15. Hausladen A, Alscher RG (1994) Cold-hardiness-specific glutathione reductase isozymes in red spruce. Plant Physiol 105: 215–223Google Scholar
  16. Hell R, Bergmann L (1988) Glutathione synthetase in tobacco suspension cultures: catalytic properties and localisation. Physiol Plant 72: 70–76Google Scholar
  17. Hell R, Bergmann L (1990) γ-Glutamylcysteine synthetase in higher plants: catalytic properties and subcellular localisation. Planta 180: 603–612Google Scholar
  18. Henschel G (1970) Untersuchungen über die Aufnahme von15N markiertem Harnstoff beiPhaseolus vulgaris L. Ph.D. thesis, University of HohenheimStuttgartGoogle Scholar
  19. Jahnke LS, Hull MR, Long SP (1991) Chilling stress and oxygen metabolising enzymes inZea mays andZea diploperennis. Plant Cell Environ 14:97–104Google Scholar
  20. Li J, Schiff JA (1991) Purification and properties of adenosine 5′-phosphosulfate sulfotransferase fromEuglena. Biochem J 274: 355–360Google Scholar
  21. Neuenschwander U, Suter M, Brunold C (1991) Regulation of sulfate assimilation by light and O-acetyl-l-serine inLemna minor L. Plant Physiol 97: 253–258Google Scholar
  22. Neyra CA, Hageman RH (1975) Nitrate uptake and induction of nitrate reductase in excised corn roots. Plant Physiol 56: 692–695Google Scholar
  23. Nussbaum S, Schmutz D, Brunold C (1988) Regulation of assimilatory sulfate reduction by cadmium inZea mays L. Plant Physiol 88: 1407–1410Google Scholar
  24. Prasad TK, Anderson MD, Martin BA, Stewart CR (1994) Evidence for chilling induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell 6: 65–74Google Scholar
  25. Rennenberg H, Brunold C (1994) Significance of glutathione meta-bolism in plants under stress. Prog Bot 55: 142–156Google Scholar
  26. Rüegsegger A, Brunold C (1992) Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings. Plant Physiol 99: 428–433Google Scholar
  27. Scandalios JG (1990) Response of plant antioxidant defence genes to environmental stress. In: Scandalios JG (ed) Genomic responses to environmental stress. Academic Press Inc, San Diego, pp 2–42Google Scholar
  28. Schupp R, Rennenberg H (1988) Diurnal changes in the glutathione content of spruce needles (Picea abies L.). Plant Sci 57: 113–117Google Scholar
  29. Smith IK, Vierheller TL, Thorne CA (1988) Assay of glutathione reductase in crude tissue homogenates using 5,5′dithiobis(2-nitrobenzoic acid). Anal Biochem 175: 408–413Google Scholar
  30. Stamp P, Geisler G, Thiraporn R (1983) Adaptation to sub- and supraoptimal temperatures of inbred maize lines differing in origin with regard to seedling development and photosynthetic traits. Physiol Plant 58: 62–68Google Scholar
  31. Stuiver CEE, de Kok LJ, Kuiper PJC (1992) Does glutathione play a role in freezing tolerance of plants? Phyton (Horn, Austria), Special issue: “Sulfur-Metabolism” 32: 139–142Google Scholar
  32. Vierheller TL, Smith IK (1990) Effect of chilling on glutathione reductase and total glutathione in soybean leavesGlycine max (L.) Merr. In: Rennenberg H, Brunold C, de Kok LJ, Stulen I (eds) Sulfur nutrition and sulfur assimilation in higher plants. SPB Academic Publishing bv, The Hague, pp 261–265Google Scholar
  33. Walker MA, McKersie BD (1993) Role of the ascorbate-glutathione antioxidant system in chilling resistance of tomato. J Plant Physiol 141: 234–239Google Scholar
  34. Wang CY (1995) Temperature preconditioning affects glutathione content and glutathione reductase activity in chilled zucchini squash. J Plant Physiol 145: 148–152Google Scholar

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Gábor Kocsy
    • 1
  • Monika Brunner
    • 1
  • Adrian Rüegsegger
    • 1
  • Peter Stamp
    • 2
  • Christian Brunold
    • 1
    Email author
  1. 1.Institute of Plant PhysiologyUniversity of BerneBerneSwitzerland
  2. 2.Institute of Plant Sciences, ETH ZurichZürichSwitzerland

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