Quantitation of phenobarbital and its main metabolites in human urine

  • N. Kållberg
  • S. Agurell
  • Ö. Ericsson
  • E. Bucht
  • B. Jalling
  • L. O. Boréus
Article

Summary

A method for the quantitative determination of phenobarbital and free and conjugatedp-hydroxyphenobarbital in urine samples is described. The method includes initial extraction, purification on a small chromatographic column and finally determination by gas chromatography. The barbituric acids are methylated by trimethylanilinium hydroxide which serves as a “flash heater” methylating agent. The conjugate ofp-hydroxyphenobarbital, which appears to be a glucuronide, is hydrolysed with hydrochloric acid.

Key words

Conjugate-cleavage drug metabolism gas chromatography phenobarbital urinary excretion 

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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • N. Kållberg
    • 1
  • S. Agurell
    • 1
  • Ö. Ericsson
    • 2
  • E. Bucht
    • 3
  • B. Jalling
    • 3
  • L. O. Boréus
    • 3
  1. 1.The Karolinska PharmacyStockholmSweden
  2. 2.Dept. of Organic Chemistry, Faculty of PharmacyUniversity of UppsalaStockholmSweden
  3. 3.Dept. of Clinical PharmacologyKarolinska HospitalStockholmSweden

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