Synovial tissue has been examined by electron microscopy from patients suffering from either sero-positive or sero-negative inflammatory arthropathies to allow direct comparison of the ultrastructural changes found in these groups and to confirm and extend observations previously made in a smaller group of sero-negative arthropathies. Both groups have been compared with material from healthy controls.
The sero-positive group comprised 13 cases of ‘definite’ or ‘classical’ rheumatoid arthritis. The sero-negative group consisted of 9 cases of arthritis secondary to Crohn's disease (3); Reiter's syndrome (2); Whipple's disease (1); Behcet's disease (1); Wegener's granulomatosis (1) and ankylosing spondylitis (1). The control tissue was obtained from 6 non-arthritic subjects undergoing surgery for non-inflammatory conditions.
Confirmation was obtained of changes previously reported in sub-cellular organelles, especially in synovial B cells, in all forms of inflammatory arthritis as compared with controls. Attention is now drawn to other intracellular changes in B cells and intermediate cells which included: a marked increase of intermediate filaments and microfilaments: and proliferation of pinocytotic vesicles and rough endoplasmic reticulum. These changes were often accompanied by the presence, in the immediate environment of these cells, of extracellular microfibrillary masses but little or no accumulation of intermediate filaments.
It was confirmed that synovial A cells were reduced in number but showed changes suggestive of increased phagocytic activity and also exhibited proliferation of cytoskeletal elements.
Differences in these structural changes between seropositive and seronegative arthritis were of degree rather than of kind and no ‘specific’ or diagnostic differences were observed between the various forms of seronegative athropathies. The possible significance of the structural changes observed is discussed.
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Barland P, Novikoff AB, Hamerman D (1962) Electron microscopy of the human synovial membrane. J Cell Biol 14:207–220
Barland P, Novikoff AB, Hamerman D (1964) Fine structure and cytochemistry of the rheumatoid synovial membrane, with special reference to Lysosomes. Am J Pathol 44:853–866
Barland P, Novikoff AB, Hamerman D (1964) Lysosomes in the synovial membrane in rheumatoid arthritis: a mechanism for cartilage erosion. Trans Ass Am Physicians 77:239
Buckley IK, Raju TR, Stewart M (1977) Some intermediate sized filaments in cultured chick embryo fibroblasts contain F-actin. J Cell Biol 75a, MF 583
Dryll, Lansaman J, Cazalis P, Peltier AP, De Seze S (1977) Light and electron microscopic study of capillaries in normal and inflammatory human synovial membrane. J Clin Pathol 30:556–562
Farr M, Morris CJ, Hollywell CA, Scott DL, Bacon PA, Walton KW (1983) Amyloidosis in Whipple's arthritis. J Roy Soc Med (in press)
Felix M, Straüli P (1976) Different distribution pattern of 100 Å filaments in resting and locomotive leukaemia cells. Nature 261:604–606
Ghadially FN, Roy S (1967) Ultrastructure of synovial membrane in rheumatoid arthritis. Ann Rheum Dis 26:426–443
Ghadially FN, Roy S (1969) Ultrastructure of synovial joints in health and disease. Butterworths, London
Goffin YA, Thona Y, Potvliege PR (1981) Microdeposition of amyloid in the joints. Ann Rheum Dis 40:27–33
Goldie I (1969) The synovial microvascular derangement in rheumatoid arthritis and osteoarthritis. Acta Orthop Scand 40:751–764
Hirohata K, Kobayashi I (1964) Fine structure of synovial tissues in rheumatoid arthritis. Kobe J Med Sci 10:195
Hynes RO, Destree AR (1978) 10 nm filaments in normal and transformed cells. Cell 13:151–163
Ishikawa H, Bischoff R, Holtzer H (1969) Formation of arrowhead complexes with heavy meromyosin in a variety of cell types. J Cell Biol 43:312–328
Jorgensen AO, Subrahmanya L, Turnbull C (1976) Localisation of the neurofilament protein in neuroblastoma cells by immunofluorescent staining. Proc Nat Acad Sci USA 73:3192–3196
Lazarides SE (1980) Intermediate filaments as mechanical integrators of cellular space. Nature 283:249–256
Lazarides E, Baltzer D Jr (1977) Heterogeneity of desmins between mammalian and avian muscles and their co-isolation with a specific isoelectric variant of actin. J Cell Biol 75a, MF 427
Lehto VP, Virtanen I, Kurki P (1978) Intermediate filaments anchor the nuclei in nuclear monolayers of cultured human fibroblasts. Nature 272:175–177
Morris CJ, Farr M, Hollywell CA, Hawkins CF, Scott DL, Walton KW (1983) Ultrastructure of the synovial membrane in seronegative inflammatory arthropathies. J Roy Soc Med 76:27–31
Norton WL, Ziff M (1966) Electron microscopic observations on the rheumatoid synovial membrane. Arthritis Rheum 9:589–610
Pollard TO, Weihing RR (1974) Actin and myosin and cell movement. CRC Crit Rev Biochem 2:1–65
Reynolds ES (1963) The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17:208–212
Small JV, Cellis JE (1978) Direct visualization of the 10 nm 100 Å filament network in whole and enucleated cultured cells. J Cell Sci 31:393–409
Spurr AR (1969) A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26:31–43
Williamson N, Holt JP (1960) A synovial biopsy needle. Lancet 1:799
Wynne-Roberts CR, Castor CW (1972) Ultrastructural comparison of rheumatoid and non rheumatoid synovial fibroblasts grown in tissue culture. Arthritis Rheum (1972) 15:65–83
Wyllie JC, Haust MD, More RH (1966) The fine structure of synovial lining cells in rheumatoid arthritis. Lab Invest 15:519–529
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Hollywell, C., Morris, C.J., Farr, M. et al. Ultrastructure of synovial changes in rheumatoid disease and in seronegative inflammatory arthropathies. Vichows Archiv A Pathol Anat 400, 345–355 (1983). https://doi.org/10.1007/BF00612195
- Cytoplasmic filaments
- Synovial cells
- Rheumatoid arthritis
- Seronegative arthropathies