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Contributions of immobilized and free cells of salt-tolerantZygosaccharomyces rouxii andCandida versatilis to the production of ethanol and 4-ethylguaiacol

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The contribution of immobilized cells and free cells released from gel beads to ethanol production by the salt-tolerant yeastsZygosaccharomyces rouxii andCandida versatilis, and 4-ethylguaiacol (4-EG) production byC. versatilis were investigated using an airlift reactor. The amounts of ethanol produced by free cells were about 65% and about 90% of total ethanol in the reactor forZ. rouxii andC. versatilis, respectively. It was found that immobilized cells gave a much lower specific productivity of ethanol (ethanol production per hour per cell) than free cells of both yeasts, especially ofC. versatilis. 4-EG was produced mainly by immobilized cells ofC. versatilis; the amount of 4-EG produced by free cells was about 20% of the total 4-EG, in contrast to the results of ethanol production. However, the specific productivity of 4-EG (4-EG production per hour per cell) by immobilized and free cells was fairly similar.

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  1. Casey GP, Ingledew WM (1986) Ethanol tolerance in yeasts. Crit Rev Microbiol 13:369–377

  2. Dombek KM, Ingram LO (1987) Ethanol production during batch fermentation withSaccharomyces cerevisiae: change in glycolytic enzymes and internal pH. Appl Environ Microbiol 53:1286–1291

  3. Hamada T, Ishiyama T, Motai H (1989) Continuous fermentation of soy sauce by immobilizedZygosaccharomyces rouxii in an airlift reactor. Appl Microbiol Biotechnol 31:346–350

  4. Hamada T, Sugishita M, Motai H (1990) Continuous production of 4-ethylguaiacol by immobilized cells of salt-tolerantCandida versatilis in an airlift reactor. J Ferment Bioeng 69:166–169

  5. Hamano M, Okuhara A, Aoyama Y, Saito N (1971) Quantitative determination of acetic acid and ethyl alcohol in soy sauce with gas liquid chromatography. Seasoning Science (Japan) 18:72–78

  6. Ingram LO, Buttke TM (1984) Effects of ethanol on microorganisms. Adv Microb Physiol 25:253–300

  7. Mori A, Matsumoto N, Imai C (1989) Growth behavior of immobilized acetic bacteria. Biotechnol Lett 11:183–188

  8. Moulin G, Boze H, Galzy P (1984) Inhibition of alcoholic fermentation. Biotechnol Bioeng 25:365–382

  9. Ohta S, Suenaga H, Nagahama M, Yamashita S, Hirano T, Yamaguchi T (1989) Production of persimmon- and kiwifruit-wine using immobilized cells. Nippon Shokuhin Kogyo Gakkaishi 36:903–909

  10. Osaki K, Okamoto Y, Akao T, Nagata S, Takamatsu H (1985) Fermentation of soy sauce with immobilized whole cells. J Food Sci 50:1289–1292

  11. Toda K, Ohtake H, Asakura T (1986) Ethanol production in horizontal bioreactor. Appl Microbiol Biotechnol 24:97–101

  12. Wada M, Kato J, Chibata I (1981) Continuous production of ethanol in high concentration using immobilized growing cells. Eur J Appl Microbiol Biotechnol 11:67–71

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Correspondence to Takashi Hamada.

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Hamada, T., Sugishita, M. & Motai, H. Contributions of immobilized and free cells of salt-tolerantZygosaccharomyces rouxii andCandida versatilis to the production of ethanol and 4-ethylguaiacol. Appl Microbiol Biotechnol 33, 624–628 (1990). https://doi.org/10.1007/BF00604926

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  • Ethanol Production
  • Specific Productivity
  • Immobilize Cell
  • Free Cell
  • Airlift Reactor