Differential mobilization of leucocyte and lymphocyte subpopulations into the circulation during endurance exercise

  • Holger Gabriel
  • Lothar Schwarz
  • Petra Born
  • Wilfried Kindermann
Article

Summary

A total of 14 healthy subjects [means (SD): 27.6 (3.8) years; body mass 77.8 (6.6) kg; height 183 (6) cm] performed endurance exercise to exhaustion at 100% of the individual anaerobic threshold (Than) on a cycle ergometer (mean workload 207 (55) W; lactate concentrations 3.4 (1.2) mmol · l−1; duration 83.8 (22.2) min, including 5 min at 50% of individual Than). Leucocyte subpopulations were measured by flow cytometry and catecholamines by radioimmunological methods. Blood samples were taken before and several times during exercise. Values were corrected for plasma volume changes and analysed using ANOVA for repeated measures. During the first 10 min of exercise, of all cell subpopulations the natural killer cells (CD3CD16/CD56+) increased the most (229%). Also CD3÷CD16/CD56+ (84%), CD8÷CD45RO (69%) cells, eosinophils (36%) and monocytes (62%) increased rapidly during thattime.CD3+, CD3+HLA-DR+, CD4+CD45RO+, CD4+CD45RO, CD8+CD45RO÷ and CD19+ cells either did not increase or increased only slightly during exercise. Adrenaline and noradrenaline increased nearly linearly by 36% and 77% respectively at 10 min exercise. The increase of natural killer cells and heart rates between rest and 10 min of exercise correlated significantly (r=0.576,P=0.031). We conclude that natural killer cells, cytotoxic, non-MHC-restricted T-cells, monocytes and eosinophils are mobilized rapidly during the first minutes of endurance exercise. Both catecholamines and increased blood flow are likely to contribute this effect.

Key words

Leucocyte subpopulations Natural killer cells Catecholamines Endurance exercise 

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References

  1. Berk LS, Niemann DC, Youngberg WS, Arabatzis K, SimpsonWesterberg M, Lee JW, Tan SA, Eby WC (1990) The effect of long endurance running on natural killer cells in marathoners. Med Sci Sports Exerc 22:207–212Google Scholar
  2. Borg GAV (1973) Perceived exertion: a note on “history” and methods. Med Sci Sports Exerc 5:90–93Google Scholar
  3. Crary B, Hauser SL, Borysenko M, Kutz I, Hoban C, Ault KA, Weiner HL, Benson H (1983) Epinephrine-induced changes in the distribution of lymphocyte subsets in peripheral blood of humans. J Immunol 131:1178–1181Google Scholar
  4. Da Prada M, Zürcher G (1976) Simultaneous radioenzymatic determination of plasma and tissue adrenaline, noradrenaline, and dopamine within the femtomole range. Life Sci 19:1161–1174Google Scholar
  5. Dill DB, Costill DL (1974) Calculation of percentage changes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248Google Scholar
  6. Gabriel H, Schwarz L, Urhausen A, Biro G, Kindermann W (1991a) 30-minute-lasting endurance exercises of different intensities and lymphocyte subpopulations. In: Bernett P, Jeschke D (eds) Sport und Medizin - Pro und Contra. Zuckschwerdt, Munich, pp 618–620Google Scholar
  7. Gabriel H, Urhausen A, Kindermann W (1991b) Circulating leucocyte and lymphocyte subpopulations before and after intensive endurance exercise to exhaustion. Eur J Appl Physiol 63:449–457Google Scholar
  8. Gabriel H, Schwarz L, Steffens G, Kindermann W (1992a) Immunoregulatory hormones, circulating leukocyte and lymphocyte subpopulations before and after endurance exercise of different intensities. Int J Sports Med 13:359–366Google Scholar
  9. Gabriel H, Urhausen A, Kindermann W (1992b) Mobilization of circulating leukocyte and lymphocyte subpopulations during and after a short, anaerobic exercise. Eur J Appl Physiol 65:164–170Google Scholar
  10. Hebermann RB (1986) Natural killer cells. Annu Rev Med 37:347–352Google Scholar
  11. Hoffmann-Goetz L, Simpson JR, Cipp N, Arumugam Y, Houston ME (1990) Lymphocyte subset responses to repeated submaximal exercise in men. J Appl Physiol 68:1069–1074Google Scholar
  12. Hohorst HJ (1962) L-(+)-Lactat, Bestimmung mit Lactatdehydrogenase und DPN. In: Bergmeyer HU (ed) Methoden der enzymatischen Analyse. Verlag Chemie, Weinheim, pp 266–277Google Scholar
  13. Kappel M, Tvede N, Galbo H, Haahr PM, Kjaer M, Linstow M, Klarlund M, Pedersen BK (1991) Evidence that the effect of physical exercise on NK cell activity is mediated by epinephrine. J Appl Physiol 70:2530–2534Google Scholar
  14. Keast D, Cameron K, Morton AR (1988) Exercise and immune response. Sports Med 5:248–267Google Scholar
  15. Kendall A, Hoffmann-Goetz L, Houston ME, MacNeil B, Arumugam Y (1990) Exercise and blood lymphocyte subset responses: intensity, duration, and subject fitness effects. J Appl Physiol 69:251–260Google Scholar
  16. Landmann RMA, Bürgisser E, Wesp M, Bühler FR (1984) Betaadrenergic receptors are different in subpopulations of human circulating lymphocytes. J Recept Res 4:37–50Google Scholar
  17. Lanier LL, Le AM, Civin CI, Loken MR, Phillips JH (1986) The relationship of CD16 (Leu-11) and Leu-19 (NKH-1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol 136:4480–4486Google Scholar
  18. Mackinnon LT (1989) Exercise and natural killer cells. What is the relationship? Sports Med 7:141–149Google Scholar
  19. Maisel AS, Harris T, Rearden CA, Michel MC (1990) ß-Adrener-gic receptors in lymphocyte subsets after exercise. Circulation 82:2003–2010Google Scholar
  20. McCarthy DA, Dale MM (1988) The leucocytosis of exercise. Sports Med 6:333–363Google Scholar
  21. Nehlsen-Cannarella SL, Nieman DC, Balk-Lanberton AJ, Markhoff PA, Arabatzis K, Chritton DBH, Gusewitch G, Le JW (1991) The effects of moderate training on immune response. Med Sci Sports Exerc 23:64–70Google Scholar
  22. Niemann DC, Berk LS, Simpson-Westerberg M, Arabatzis K, Youngberg WS, Tan SA, Lee JW, Eby WC (1989) Effects of long endurance running on immune system parameters and lymphocyte function in experienced marathoners. Int J Sports Med 10:317–323Google Scholar
  23. Niemann DC, Nehlsen-Cannarella SL, Donohue KM, Chritton DBH, Haddock BL, Stout RW, Lee JW (1991) The effect of acute moderate exercise on leukocyte and lymphocyte subpopulations. Med Sci Sports Exerc 23:578–585Google Scholar
  24. Pedersen BK, Tvede N, Klarlund K, Christensen LD, Hansen FR, Galbo H, Kharazmi A, Halkjaer-Kristensen J (1990) Indomethacin in vitro and in vivo abolishes post exercise suppression of natural killer cell activity in peripheral blood. Int J Sports Med 11:127–131Google Scholar
  25. Shephard RJ, Verde TV, Thomas SG, Shek P (1991) Physical activity and the immune system. Can J Sport Sci 16:163–185Google Scholar
  26. Stauber WT, Fritz VK, Vogelbach DW, Dahlmann B (1988) Characterization of muscle injured by forced lengthening. I. Cellular infiltrates. Med Sci Sports Exerc 20:345–353Google Scholar
  27. Stegmann H, Kindermann W, Schnabel H (1981) Lactate kinetics and individual anaerobic threshold. Int J Sports Med 2:160–165Google Scholar
  28. Tvede N, Pedersen BK, Hansen FR, Bendix T, Christensen LD, Galbo H, Halkjaer-Kristensen J (1989) Effect of physical exercise on blood mononuclear cell subpopulations and in vitro proliferative responses. Scand J Immunol 29:383–389Google Scholar
  29. Van Tits LJ, Michel MC, Grosse-Wilde H, Happel M, Eigler FW, Soliman A, Brodde OE (1990) Catecholamines increase lymphocyte β2-adrenergic receptors via a β2-adrenergic, spleen-dependent process. Am J Physiol 258:E191-E202Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Holger Gabriel
    • 1
  • Lothar Schwarz
    • 1
  • Petra Born
    • 1
  • Wilfried Kindermann
    • 1
  1. 1.Institute of Sports and Performance MedicineUniversity of SaarlandSaarbrückenGermany

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