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Analysis of oligospirostanosides in a suspension culture of Dioscorea deltoidea cells by high-performance liquid chromatography

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Abstract

A method has been developed for the quantitative determination of oligospirostanosides in a culture ofDioscorea deltoidea cells with the aid of HPLC on a LiChrosorb RP-18 column. Elution was performed with acetonitrile-water (50–75%) with detection at 207 nm.

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Additional information

A. N. Bakh Institute of Biochemistry, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 549–551, July–August, 1988.

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Paukov, V.N., Vasil'eva, I.S., Karasev, N.N. et al. Analysis of oligospirostanosides in a suspension culture of Dioscorea deltoidea cells by high-performance liquid chromatography. Chem Nat Compd 24, 465–467 (1988). https://doi.org/10.1007/BF00598533

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Keywords

  • Suspension Culture
  • Lactam
  • Vindoline
  • Dioscin
  • Steroid Glycoside