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Biosynthesis of collagen and elastin by chick embryo skin during maturation

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Summary

The biosynthess of collagen and elastin by chick embryo skin of 11–16 days of development was studied. An increased uptake of (14C) proline and (14C) lysine on day 12 was observed in collagen peptides and in elastin extracted from the skin samples.

(14C)hydroxyproline followed the same pattern as the uptake of its precursor into the collagenase-digested peptide fraction. The conversion of (14C)lysine into (14C)hydroxylysine was low. The conversion of (14C)glutamic acid and (14C)glutamine into (14C)proline and (14C)hydroxyproline by the skin of chicken embryos may be an important pathway.

As far as (14C)proline and (14C)lysine is concerned elastin showed a similar pattern of changes during the maturation process.

Zusammenfassung

Die Kollagen- und Elastinbiosynthese in 11–16 Tage alter Hühnchenhaut wurde untersucht. Am 12. Tag zeigte sich eine erhöhte Aufnahme von14C-Prolin und C14-Lysin in Kollagenpeptide und Elastin, die aus den Hautpräparaten isoliert wurden. C14-Hydroxyprolin zeigte ein gleiches Muster des Einbaues in die kollagenase-verdauliche Peptidfraktion. Die Konversion von14C-Lysin in14C-Hydroxylysin war gering. Der Übergang von C14-Glutaminsäure und C14-Glutamin in14C-Prolin und14C-Hydroxyprolin in der Hühnchenhaut ist möglicherweise eine wichtige Route. Elastin zeigte hinsichtlich C14-Prolin und C14-Lysin während des Reifungsprozesses ein ähnliches Einbauverhalten.

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References

  1. 1.

    Blumenkrantz, N., Asboe-Hansen, G.: Biosynthesis of collagen (14C)hydroxyproline and (14C)hydroxylysine during maturation of chick embryos. Mech. Age. Dev.1, 445 (1972/1973)

  2. 2.

    Blumenkrantz, N., Prockop, D. J.: A rapid assay for14C-labelled hydroxylysine in collagen and related materials. Analyt. Biochem.30, 377 (1969)

  3. 3.

    Boucek, R. J., Hlavackova, V.: Synthesis and degradation of collagen in the developing corium of the chick embryo. Biochem. J.110, 435 (1968)

  4. 4.

    Bruzuszkiewicz-Zarnowska, H.: Glutamic acid participation in the process of collagen biosynthesis. Bull. Acad. pol. Sci.19, 167 (1971)

  5. 5.

    Fitzpatrick, M., Hospelhorn, V. D.: Changes in amino acid composition of aortic elastin with ageing and arteriosclerosis. Amer. Heart J.69, 211 (1965)

  6. 6.

    Halme, J.: Development of protocollagen proline hydroxylase activity in the chick embryo. Biochim. biophys. Acta (Amst.)192, 90 (1969)

  7. 7.

    Ishibashi, S., Ide, T., Tsurufuji, S.: Role of glutamic acid as a precursor of collagen proline and hydroxyproline. Biochim. biophys. Acta (Amst.)165, 296 (1968)

  8. 8.

    Juva, K., Prockop, D. J.: Modified procedure for the assay of H-3 or C-14-labelled hydroxyproline. Analyt. Biochem.15, 77 (1966)

  9. 9.

    Kivirikko, K. J.: Hydroxyproline-containing fractions in normal and cortisonetreated chick embryos. Acta physiol. scand., Suppl.219, 92 (1963)

  10. 10.

    Neuman, R. E.: Hydroxyproline content in the developing chick embryo. Proc. Soc. exp. Biol. (N.Y.)75, 37 (1950)

  11. 11.

    Prockop, D. J., Juva, K.: Synthesis of hydroxyprolinein vitro by the hydroxylation of proline in a precursor of collagen. Proc. nat. Acad. Sci. (Wash.)53, 661 (1965)

  12. 12.

    Sandberg, L. B., Cleary, E. G.: Pattern of lysine incorporation into elastin of growing chick aorta. Biochim. biophys. Acta (Amst.)154, 411 (1968)

  13. 13.

    Schiller, S., Slover, G. A., Dorfman, A.: A method for the separation of acid mucopolysaccharides: Its application to the isolation of heparin from the skin of rats. J. biol. Chem.236, 983 (1961)

  14. 14.

    Uitto, J.: A method for studying collagen biosynthesis in human skin biopsiesin vitro. Biochim. biophys. Acta (Amst.)201, 438 (1970)

  15. 15.

    Woessner, J. F., Jr., Bashey, R. I., Boucek, R. J.: Collagen development in heart and skin of the chick embryo. Biochim. biophys. Acta (Amst.)140, 329 (1967)

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Blumenkrantz, N., Asboe-Hansen, G. Biosynthesis of collagen and elastin by chick embryo skin during maturation. Arch. Derm. Forsch. 249, 99–104 (1974). https://doi.org/10.1007/BF00595571

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Keywords

  • Collagen
  • Peptide
  • Proline
  • Lysine
  • Glutamine