Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Collagenolytic-like activity of normal human epidermis

  • 15 Accesses

  • 2 Citations


A protease with a bacterial-like collagenolytic activity was demonstrated in epidermal homogenates of 7 normal human volunteers, using the epidermis of suction-induced blisters and a synthetic chromophore substrate. The enzyme was quantitated and expressed in terms of an equivalent amount of bacterial collagenase.

The relationship of these proteases to the different collagenases is discussed.


Eine Protease mit bakterienähnlicher collagenolytischer Aktivität wurde in Epidermishomogenaten aus Saugblasen von 7 Normalpersonen unter Verwendung eines synthetischen Chromophorensubstrates gefunden.

Die Enzymaktivität wurde quantitativ in Äquivalentmengen zu bakterieller Collagenase bestimmt. Das Verhältnis dieser Protease zu den verschiedenen Collagenasen wird diskutiert.

This is a preview of subscription content, log in to check access.


  1. 1.

    Eisen, A. Z.: Human skin collagenase: Localization and distribution in normal human skin. J. invest. Derm.52, 442–448 (1969).

  2. 2.

    —, Jeffrey, J. J., Gross, J.: Human skin collagenase. Isolation and mechanism of attack on the collagen molecule. Biochim. biophys. Acta (Amst.)151, 637–645 (1968).

  3. 3.

    Gries, G., Buresch, H., Strauch, L.: Collagenolytic enzymes in human serum. Experientia (Basel)26, 31–33 (1970).

  4. 4.

    Grillo, H. C., Gross, J.: Collagenolytic activity and epithelial-mesenchymal interactions in healing mammalian wounds. Paper from platform. J. Cell Biol.23, 39A (1964).

  5. 5.

    —, —: Collagenolytic activity during mammalian wound repair. Develop. Biol.15, 300–317 (1967).

  6. 6.

    Gross, J., Lapiere, C. M.: Collagenolytic activity in amphibian tissues: A tissue culture assay. Proc. nat. Acad. Sci.48, 1014–1022 (1962).

  7. 7.

    Harper, E., Gross, J.: Separation of collagenase and peptidase activities of tadpole tissues in culture. Biochim. biophys. Acta (Amst.)198, 286–292 (1970).

  8. 8.

    Heidrich, H. G., Prokopova, D., Hannig, K.: The use of synthetic substrates for the determination of mammalian collagenases. Hoppe-Seylers Z. physiol. Chem.350, 1430–1436 (1969).

  9. 9.

    Kang, A. H., Nagai, Y., Piez, K. A., Gross, J.: Studies on the structure of collagen utilizing a collagenolytic enzyme from tadpole. Biochemistry5, 509–515 (1966).

  10. 10.

    Kiistala, U.: Suction blister device for separation of viable epidermis from dermis. J. invest. Derm.50, 129–137 (1968).

  11. 11.

    —, Mustakallio, K. K.: Dermo-epidermal separation with suction. Electron microscopic and histochemical study of initial events of blistering on human skin. J. invest. Derm.48, 466–477 (1967).

  12. 12.

    Kono, T.: Purification of bacterial collagenolytic enzymes. Fed. Proc.26, 670 (1967).

  13. 13.

    —: Purification and partial characterization of collagenolytic enzymes from Clostridium histolyticum. Biochemistry7, 1106–1114 (1968).

  14. 14.

    Lapiere, C. M., Gross, J.: Animal collagenase and collagen metabolism. In: Mechanisms of hard tissue destruction. Publ. No. 75, pp. 663–694. Am. Assoc. Advance. Sci. Washington D.C. (1963).

  15. 15.

    Lazarus, G. S., Fullmer, H. M.: Collagenase production by human dermis in vitro. J. invest. Derm.52, 545–547 (1969).

  16. 16.

    Nagai, Y., Lapiere, C. M., Gross, J.: Tadpole collagenase. Preparation and purification. Biochemistry5, 3123–3130 (1966).

  17. 17.

    —, Sakakibara, S., Noda, H., Akabori, S.: Hydrolysis of synthetic peptides by collagenase. Biochim. biophys. Acta (Amst.)37, 567–569 (1960).

  18. 18.

    Sakai, T., Gross, J.: Some properties of the products of reaction of tadpole collagenase with collagen. Biochemistry6, 518–527 (1967).

  19. 19.

    Strauch, L., Vencelj, H.: Collagenase in mammalain cells. Hoppe-Seylers Z. physiol. Chem.348, 465–468 (1967).

  20. 20.

    Waldvogel, F. A., Swartz, M. N.: Collagenolytic activity of bacteria. J. Bact.98, 662–667 (1969).

  21. 21.

    Wünsch, E., Heidrich, H. G.: Zur quantitativen Bestimmung der Kollagenase. Hoppe-Seylers Z. physiol. Chem.333, 149–151 (1963).

Download references

Author information

Additional information

Supported by a grant from the Research Committee, Faculties of Medical Sciences, American University of Beirut.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kaidbey, K.H., Kurban, A.K. & Tenekjian, K. Collagenolytic-like activity of normal human epidermis. Arch. Derm. Forsch. 242, 1–5 (1971). https://doi.org/10.1007/BF00595284

Download citation


  • Public Health
  • Enzyme
  • Collagenase
  • Equivalent Amount
  • Human Volunteer