In glycerol-extracted insect fibrillar muscle suspended in ATP salt solution the incorporation of32Pi into ATP was studied during the performance of positive or negative oscillatory work and under a variety of mechanical and ionic conditions. An increase in calcium ion concentration from 10−8–10−5 M increased the incorporation rate in proportion to the increase in ATPase activity, mean tension and immediate stiffness, which is a measure of the extent of actin-myosin interaction. Sinusoidal stretches (at 1% Lo) performed at 5 Hz induced the fibres to perform optimal positive oscillatory work and it caused a doubling of the incorporation rate (and ATPase activity). A decrease or increase of the frequency below or above the optimum of 5 Hz always decreased the power output as well as the incorporation rate which, however, was still noticeable even under conditions where work was doneon the fibres. A similar frequency dependence was found when square-wave rather than sinusoidal stretches were applied and this effect could be related to the finding that the rate of stretch-induced incorporation was highest shortly after stretching and then declined to low values (after about 100 ms). These results suggest the formation of an energy-rich intermediate (actomyosin-ADP?) during the contraction process induced by stretching and this intermediate must be assumed to accumulate transiently after stretching.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Gillis, J. M., Maréchal, G.: Influence of tension on the incorporation of inorganic phosphate in glycerinated muscle fibres. J. Physiol. (Lond.)214, 41 P (1971)
Gillis, J. M., Maréchal, G.: The incorporation of radioactive phosphate into ATP in glycerinated fibres stretched or released during contraction. J. Mechanochem. Cell Motil.3, 55–68 (1974)
Herzig, J. W.: Mode of stretch activation in heart muscle. Pflügers Arch.355, Suppl. R 13 (1975)
Hill, A. V., Howarth, J. V.: The reversal of chemical reactions in contracting muscle during an applied stretch. Proc. roy. Soc. B151, 169–193 (1959)
Hotta, K., Fujita, Y.: On the intermediate complex of actomyosin ATPase. Physiol. Chem. Physics3, 196–204 (1971)
Huxley, A. F., Simmons, R. M.: Proposed mechanism of force generation in striated muscle. Nature (Lond.)233, 533–538 (1971)
Jewell, B. R., Rüegg, J. C.: Oscillatory contraction of insect fibrillar muscle after glycerol extraction. Proc. roy. Soc. B164, 428–459 (1966)
Lymn, R. W., Taylor, E. W.: Mechanism of adenosine triphosphate hydrolysis by actomyosin. Biochemistry10, 4617–4624 (1971)
Mannherz, H. G.: On the reversibility of the biochemical reactions of muscular contraction during the absorption of negative work. FEBS Letters10, 233–236 (1970)
Pybus, J., Tregear, R. T.: The relationship of adenosine triphosphatase activity to tension and power output of insect flight muscle. J. Physiol. (Lond.)247, 71–89 (1975)
Schädler, M., Steiger, G. J., Rüegg, J. C.: Mechanical activation and isometric oscillation in insect fibrillar muscle. Pflügers Arch.330, 217–229 (1971)
Steiger, G. J., Rüegg, J. C.: Energetics and “efficiency” in the contractile machinery of an insect fibrillar muscle at various frequencies of oscillation. Pflügers Arch.307, 1–21 (1969)
Trentham, D. R., Bardsley, R. G., Eccleston, J. F., Weeds, A. G.: Elementary process of the magnesium-ion-dependent adenosine triphosphatase activity of heavy meromyosin. Biochem. J.126, 635–644 (1972)
Ulbrich, M.: Wirkung mechanischer Faktoren auf die Inkorporation von32P-Phosphat in Adenosintriphosphat bei glycerin-extrahierten Insektenmuskeln. (Untersuchungen über die Reversibilität der chemomechanischen Energietransformation). Thesis, Bochum 1975
Ulbrich, M., Paulsen, G.: Bildung eines energiereichen Aktomyosin-ADP-Komplexes in extrahierten Muskelfasern und isolierten Proteinen. Hoppe-Seylers Z. physiol. Chem.354, 232 (1973)
Ulbrich, M., Rüegg, J. C.: Stretch induced formation of ATP-32P in glycerinated fibres of insect flight muscle. Experientia (Basel)27, 45–46 (1971)
Wolcott, R. G., Boyer, P. D.: The reversal of the myosin and actomyosin ATPase reactions and the free energy of ATP binding to myosin. Biochem. biophys. Res. Commun.57, 709–716 (1974)
Supported by a grant from the Deutsche Forschungsgemeinschaft (Ru 154, 8–10).
About this article
Cite this article
Ulbrich, M., Rüegg, J.C. Is the chemomechanical energy transformation reversible?. Pflugers Arch. 363, 219–222 (1976). https://doi.org/10.1007/BF00594604
- Insect flight muscle
- ATP-Phosphate exchange
- Contractile mechanism
- Calcium, activition of
- Chemo-mechanical energy transformation, reversibility of