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Arachidonic acid metabolism in human skin fibroblast cultures

Arachidonsäurestoffwechsel in menschlichen Hautfibroblasten-Kulturen

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Our ongoing study shows that HSF cultures can be used as an in vitro model for AA metabolism studies. The incubation medium however has to be standardised in order to obtain comparable results. The addition of albumin to the medium is necessary for dissolution of different agents like ionophore, indomethacin or others and does not change the AA metabolism. Differences between different cell lines of healthy donors are under investigation but seem to be of minor importance when the skin biopsie is taken from the same place, whereas greater differences were observed between HSF cultured from arm or foreskin biopsies. Finally it could be proved that the cyclooxygenase pathway is more important than that of the lipoxygenase and that 11-HETE derives from the cyclooxygenase and not from the lipoxygenase activity. The main lipoxygenase product is 15-HETE.

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  1. 1.

    Bryant RW, Feinmark SJ, Malteja AN, Bailey JM (1978) J Biol Chem 253:8134–8142

  2. 2.

    Folch J, Lees M, Stanley GH (1957) J Biol Chem 226:497–512

  3. 3.

    Hopkins NK, Sun F, Gorman RR (1978) Biochem Biophys Res Commun 85:827–836

  4. 4.

    Porter NA, Wolf RA, Yarbro EM, Weenen H (1979) Biochem Biophys Res Commun 89:1058–1064

  5. 5.

    Taylor L, Polgar P (1981) Prostaglandins 22:723–728

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Correspondence to H. Gleispach.

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Gleispach, H., Mayer, B., Moser, R. et al. Arachidonic acid metabolism in human skin fibroblast cultures. Z. Anal. Chem. 317, 740–741 (1984). https://doi.org/10.1007/BF00593893

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  • Arachidonic Acid
  • Indomethacin
  • Acid Metabolism
  • Human Skin
  • Healthy Donor