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Molecular mapping of the immunologic surface of the hCG molecule by monoclonal antibodies (MCA)

Development of 3 different, epitope-selective immunoradiometric assays (IRMA) for measurement of holo-hCG and its free subunits in normal subjects, carcinoma patients and carcinoma cell line cultures

Vermessung der immunologischen Oberfläche des hCG-Moleküls durch monoklonale Antikörper (MCA) —

Entwicklung von 3 verschiedenen epitop-selektiven immunoradiometrischen Assays (IRMA) zur Messung von Holo-hCG und seinen freien Untereinheiten in normalen Individuen, Carcinom-Patienten und Carcinom-Zellkulturen

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Conclusion

The 2-site IRMA-design with MCA is demonstrated to be superior over conventional “l-site”-RIA with polyclonal antibodies since it clearly distinguishes different molecular species of a hormone, especially hCG. Such parallel measurements are considered to be of diagnostic value for screening and therapeutic monitoring of tumor patients who produce freeα- and/or freeβ-subunits early in the course of the disease, or exclusively throughout or in addition to holo-hCG.

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References

  1. Kofler R, Berger P, Wick G (1982) Am J Reprod Immunology 2:212–216

  2. Kofler R, Kalchschmid E, Berger P, Wick G (1981) Immunobiol 160:196–207

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Author information

Correspondence to S. Schwarz.

Additional information

Supported by the Austrian Cancer Research Fund 1982/83 and in part by the Österreichische Fonds zur Förderung der wissenschaftlichen Forschung, P.4597, 1983

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Schwarz, S., Berger, P. & Wick, G. Molecular mapping of the immunologic surface of the hCG molecule by monoclonal antibodies (MCA). Z. Anal. Chem. 317, 727 (1984). https://doi.org/10.1007/BF00593881

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Keywords

  • Physical Chemistry
  • Analytical Chemistry
  • Monoclonal Antibody
  • Inorganic Chemistry
  • Polyclonal Antibody