Pflügers Archiv

, Volume 338, Issue 2, pp 133–157 | Cite as

Action of a serum protein on muscular contraction

  • Anwar A. Hakim
Article
Received:

Summary

The mechanism of action of a “serum protein” isolated from human serum was assessed in several experimental preparations including glycerol-treated muscle fibers, rat heart papillary muscle and isolatedin vitro perfused rat heart. The action of the “serum protein” was studied also on canine and human heart papillary muscles which were made to respond to electrical stimulation with “ultrasonication modified epinephrine”. In addition the action of the protein on adenosine 5′ triphosphate generated precipitation of purified human actomyosin was investigated.

The “serum protein” enhanced and intensified the generation of ATP induced tension in glycerol-extracted muscle fibers. It intensified the developed tension (DT) and increased the rate of development of tension (dT/dt) without influencing the time peak tension (TPT) of capillary muscles from rat, canine and human hearts in response to electrical stimulation. The “serum protein” increased the force of contraction of the isolatedin vitro perfused rat heart, and accelerated the adenosine 5′ triphosphate generated precipitation of purified human heart actomyosin.

Key words

Serum Protein Serum Enzyme Isometric Contraction Papillary Muscle Potentiation of Excitation Contraction Serum Enhancing Enzyme Human Papillary Muscle 
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Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • Anwar A. Hakim
    • 1
  1. 1.The Biochemistry Research Laboratories of the I.T.R. Department of Preventive MedicineDMP School of Medicine University of Illinois at the Medical CenterChicagoUSA

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