, Volume 71, Issue 1, pp 81–88 | Cite as

Localization of gold in biological tissue

A photochemical method for light and electronmicroscopy
  • G. Danscher


A detailed description is given of a method by which gold can be visualized in frozen, paraffin and Epon sections. Histological sections from animals treated with gold compounds are exposed to UV-light from 30 min to several hours. The reduced, metallic gold is then visualized by means of a photographic developer containing silver lactate. Light- and electronmicroscope photographs showing gold in different organs from rats and mice treated with aurothioglucose, aurothiosulfate and aurothiomalate are presented.


Public Health Gold Lactate Paraffin Biological Tissue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Blackstad TW (1975) Electron microscopy of experimental axonal degeneration in photochemically modified Golgi preparations: A procedure for precise mapping of nervous connections. Brain Res 95:191–210Google Scholar
  2. Danscher G (1980) Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electronmicroscopy. (In preparation)Google Scholar
  3. Danscher G, Hansen HJ (1980) X-ray microprobe analysis of tissues from rats and mice intravitally treated with gold and silver. (In preparation)Google Scholar
  4. Doré JL (1974) The demonstration and distribution of gold in tissue sections. Thesis (London)Google Scholar
  5. Doré JL, Vernon-Roberts BA (1976) A method for the selective demonstration of gold in tissue sections. Med Lab Sci 33:209–213Google Scholar
  6. Gilg E (1952) A photochemical method for microdetection of gold in tissue sections. Acta Psychiatr Scand 27:43–56Google Scholar
  7. Gottlieb LN, Smith PM, Smith EM (1972) Tissue gold concentration in a rheumatoid arthristic receiving chrysotherapy. Arthritis and Rheumatism 15:16–22Google Scholar
  8. Jessop JD, Vernon-Roberts B, Harris J (1973) Effects of gold salts and prednisolone on inflammatory cells. Ann Rheum Dis 32:294–300Google Scholar
  9. Liesegang RE, Rieder W (1921) Versuche mit einer “Keimmethode” zum Nachweis von Silber in Gewebsschnitten. Z Wiss Mikrosk 38:334–338Google Scholar
  10. Persillin RH, Ziff M (1966) The effect of gold salt on lysosomal enzymes of the peritoneal macrophage. Arthritis and Rheumatism 9:57–65Google Scholar
  11. Pillai CKS, Nandi US (1973) Binding of gold (III) with DNA. Biopolymers 12:1431–1435Google Scholar
  12. Puddephatt RS (1978) The chemistry of gold. Top Org Gen Chem [NY] 16:160Google Scholar
  13. Querido A (1947) Gold intoxication of nervous elements. On the permeability of the blood-brain-barrier. Acta Psychiatr 12:151Google Scholar
  14. Roberts WJ (1935) A new procedure for the detection of gold in animal tissues. Proc R Acad [Amsterdam] 38:540–544Google Scholar
  15. Vernon-Roberts B, Doré JL, Jessop JD, Henderson WJ (1976) Selective concentration and localization of gold in macrophages of synovial and other tissues during and after chrysotherapy in rheumatoid patients. Ann Rheum Dis 35:477–486Google Scholar

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • G. Danscher
    • 1
  1. 1.Institute of Anatomy BUniversity of AarhusAarhus CDenmark

Personalised recommendations