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Plasma membranes of the kidney

III. Influence of diuretics on ATPase activity

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The effects of furosemide, amiloride and ethacrynic acid on ATPase activity in rat renal plasma membranes were studiedin vivo andin vitro. Furosemide increased volume and electrolyte content of the urine but had no influence on ATPase activity. Amiloride produced a moderate natriuresis and antikaliuresis and inhibited both Mg-ATPase and NaK-ATPase activity. Ethacrynic acid, that was active as a diuretic only after intravenous injection had no influence on NaK-ATPase activity.In vitro at concentrations of 1 mM, furosemide had no influence on ATPase activity, amiloride inhibited Mg-ATPase, NaK-ATPase and K+-activated AcPase activity. In kinetic studies the mechanism of inhibition of total ATPase activity was further characterized. No consistent relationship was observed between inhibition of Na+ reabsorption and inhibition of plasma membrane NaK-ATPase. Thus, it was concluded that these diuretics do not act by inhibiting plasma membrane NaK-ATPase.

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Ebel, H., Ehrich, J., de Santo, N.G. et al. Plasma membranes of the kidney. Pflugers Arch. 335, 224–234 (1972). https://doi.org/10.1007/BF00592159

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Key words

  • Renal Plasma Membranes
  • Diuretics
  • NaK-ATPase