Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effect of internal calcium concentration on calcium currents in rat sensory neurones

  • 26 Accesses

  • 57 Citations

Abstract

Using the patch-clamp technique in whole-cell configuration we have investigated the effect of increasing the internal calcium concentration (Cai) from below 10−8 M to 10−6 M on the three calcium currents: ICa, T (T for transient), ICa, S (S for sustained), ICa, N (N for neither), recently described in rat sensory neurones> Increasing Cai led to a dose-dependent reduction of the amplitude of ICa, S and, as Cai reached 5×10−7M ICa, S was nearly abolished. ICa, N is well evidenced from 5×10−10 M to 10−7 M where its is a large current. Preliminary observations indicate an increase of its inactivation rate following, as expected for a possible Cai dependent-inactivation, the increase of Cai from 5×10−10 M to 10−7 M. With Ca=5×10−7 M, all the cells displayed ICa, T and half of the cells in addition ICa, N, but it was of small amplitude. At Cai=10−6 M, most of the recorded cells only exhibited ICa, T.

This is a preview of subscription content, log in to check access.

References

  1. 1

    Bossu, J.-L., Feltz, A., Thomann, J.-M.: Depolarization elicits two distinct calcium currents. Pflügers Arch.403, 360–368 (1985)

  2. 2

    Bossu, J.-L., Feltz, A.: Inactivation of the low-threshold transient calcium current in rat sensory neurones: Evidence for a dual process. J. Physiol. (Lond.) in the press

  3. 3

    Carbone, E., Lux, H.D.: A low voltage-activated, fully inactivating Ca channel in vertebrate sensory neurones. Nature310, 501–502 (1984)

  4. 4

    Eckert, R., Chad, J.D.: Inactivation of Ca channels. Progress in Biophysiocs and Molecular Biology44, 215–267 (1984)

  5. 5

    Fedulova, S.A., Kostyuk, P.G., Veselovsky, N.S.: Two types of calcium channels in the somatic membrane of new-born rat dorsal root ganglion neurones. J. Physiol. (Lond.)359, 431–446 (1985)

  6. 6

    Lee, K.S., Marban, E., Tsien, R.W.: Inactivation of calcium channels in mammalian heart cells: joint dependence on membrane potential and intracellular calcium. J. Physiol. (Lond.)364, 395–411 (1985)

  7. 7

    Nowycky, M.C., Fox, A., Tsien R.W.: Three types of neuronal calcium channel with different calcium agonist sensitivity Nature316, 440–443 (1985)

  8. 8

    Reuter, H.: Calcium channel modulation by neurotransmitters, enzymes and drugs. Nature301, 569–574 (1983)

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Dupont, J.-., Bossu, J.-. & Feltz, A. Effect of internal calcium concentration on calcium currents in rat sensory neurones. Pflugers Arch. 406, 433–435 (1986). https://doi.org/10.1007/BF00590950

Download citation

Key words

  • Ca currents
  • internal Ca2+
  • sensory neurones