Antonie van Leeuwenhoek

, Volume 54, Issue 6, pp 567–573 | Cite as

Low- and intermediate-copy-number cloning vectors based on thePseudomonas plasmid pVS1

  • Y. Itoh
  • L. Soldati
  • T. Leisinger
  • D. Haas
Short Communication


The cloning vector pME290 (6.8 kb), which is derived from thePseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1–3 copies) and pME294 (about 15–20 copies) were isolated. These vectors were used in the characterization of theP. aeruginosa argF gene encoding ornithine carbamoyltransferase.

Key words

Pseudomonas pVS1 derivatives low-copy-number cloning vectors 


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Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Y. Itoh
    • 1
  • L. Soldati
    • 2
  • T. Leisinger
    • 2
  • D. Haas
    • 2
  1. 1.Dept. of BacteriologyShinshu University School of MedicineMatsumotoJapan
  2. 2.Mikrobiologisches InstitutEidgenössische Technische Hochschule ZürichZürichSwitzerland

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