Antonie van Leeuwenhoek

, Volume 54, Issue 6, pp 567–573 | Cite as

Low- and intermediate-copy-number cloning vectors based on thePseudomonas plasmid pVS1

  • Y. Itoh
  • L. Soldati
  • T. Leisinger
  • D. Haas
Short Communication

Abstract

The cloning vector pME290 (6.8 kb), which is derived from thePseudomonas plasmid pVS1 and has about 7 copies, was mutagenized in vitro to provide derivatives with altered copy numbers. Thus, pME292 (about 1–3 copies) and pME294 (about 15–20 copies) were isolated. These vectors were used in the characterization of theP. aeruginosa argF gene encoding ornithine carbamoyltransferase.

Key words

Pseudomonas pVS1 derivatives low-copy-number cloning vectors 

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References

  1. Bagdasarian M.M., E. Amann, R. Lurz, B. Rückert & M. Bagdasarian (1983) Activity of the hybridtrp-lac(tac) promoter ofEscherichia coli inPseudomonas putida. Construction of broadhost-range, controlled-expression vectors. Gene 26: 273–282Google Scholar
  2. De Reuse H., E. Touati, P. Glaser & A. Danchin (1986) Low copy number plasmid vectors for gene cloning and for monitoring gene expression. FEMS Microbiol. Lett. 37: 193–196Google Scholar
  3. Haas D., B.W. Holloway, A. Schamböck & T. Leisinger (1977) The genetic organization of arginine biosynthesis inPseudomonas aeruginosa. Mol. Gen. Genet. 154: 7–22Google Scholar
  4. Hashimoto-Gotoh T., F.C.H. Franklin, A. Nordheim & K.N. Timmis (1981) Specific-purpose plasmid cloning vectors. I. Low copy number, temperature-sensitive, mobilization-defective pSC101-derived containment vectors. Gene 16: 227–235Google Scholar
  5. Humphreys G.O., G.A. Willshaw, H.R. Smith & E.S. Anderson (1976) Mutagenesis of plasmid DNA with hydroxylamine: Isolation of mutants of multi-copy plasmids. Mol. Gen. Genet. 145: 101–108Google Scholar
  6. Isaac J. H. & B.W. Holloway (1972) Control of arginine biosynthesis inPseudomonas aeruginosa. J. Gen. Microbiol. 73: 427–438Google Scholar
  7. Itoh Y., J.M. Watson, D. Haas & T. Leisinger (1984) Genetic and molecular characterization of thePseudomonas plasmid pVS1. Plasmid 11: 206–220Google Scholar
  8. Itoh Y. & D. Haas (1985) Cloning vectors derived from thePseudomonas plasmid pVS1. Gene 36: 27–36Google Scholar
  9. Jeenes D.J., L. Soldati, H. Baur, J.M. Watson, A. Mercenier, C. Reimmann, T. Leisinger & D. Haas (1986) Expression of biosynthetic genes fromPseudomonas aeruginosa andEscherichia coli in the heterologous host. Mol. Gen. Genet. 203: 421–429Google Scholar
  10. King A., K. Shannon & I. Phillips (1988) In vitro antibacterial activity and susceptibility of cefsulodin, an antipseudomonal cephalosporin, to beta-lactamases. Antimicrob. Agents Chemother. 17: 165–169Google Scholar
  11. Leisinger T., D. Haas & M.P. Hegarty (1972) Indospicine as an arginine antagonist inEscherichia coli andPseudomonas aeruginosa. Biochim. Biophys. Acta 262: 214–219Google Scholar
  12. Miller R.V. & T.A. Kokjohn (1987) Cloning and characterization of thecI repressor ofPseudomonas aeruginosa bacteriophage D3: a functional analog of phage lambdacI protein. J. Bacteriol. 169: 1847–1852Google Scholar
  13. Philippon A., G. Paul & P. Nevot (1981) Comparative in-vitro activity of cefsulodin and ceftazidime against ticarcillin-resistantPseudomonas aeruginosa. J. Antimicrob. Chemother. 8 Suppl. B: 119–122Google Scholar
  14. Rella M. & D. Haas (1982) Resistance ofPseudomonas aeruginosa PAO to nalidixic acid and low levels of β-lactam antibiotics: mapping of chromosomal genes. Antimicrob. Agents Chemother. 22: 242–249Google Scholar
  15. Som T. & J.-I. Tomizawa (1983) Regulatory regions of ColEl that are involved in determination of plasmid copy number. Proc. Natl. Acad. Sci. USA 80: 3232–3236Google Scholar
  16. Stanisich V.A. & B.W. Holloway (1972) A mutant sex factor ofPseudomonas aeruginosa. Genet. Res. 19: 91–108.Google Scholar
  17. Voellmy R. & T. Leisinger (1978) Regulation of enzyme synthesis in the arginine biosynthetic pathway ofPseudomonas aeruginosa. J. Gen. Microbiol. 109: 25–35Google Scholar
  18. Uhlin B.E. & K. Nordström (1977) R Plasmid gene dosage effects inEscherichia coli K-12: Copy mutants of the R plasmid Rldrd-19. Plasmid 1: 1–7Google Scholar
  19. Wang K., S.E. Stachel, B. Timmerman, M. Van Montagu & P.C. Zambryski (1987) Site-specific nick in the T-DNA border sequence as a result ofAgrobacterium vir gene expression. Science 235: 587–591Google Scholar

Copyright information

© Kluwer Academic Publishers 1988

Authors and Affiliations

  • Y. Itoh
    • 1
  • L. Soldati
    • 2
  • T. Leisinger
    • 2
  • D. Haas
    • 2
  1. 1.Dept. of BacteriologyShinshu University School of MedicineMatsumotoJapan
  2. 2.Mikrobiologisches InstitutEidgenössische Technische Hochschule ZürichZürichSwitzerland

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