Uptake of Ca2+ by vesicle preparation of chick intestinal brush-border membranes was rapid and extensive. With tracer quantities of Ca2+ uptake was complete in 10 min whereas with 2.0 mM Ca2+ maximum uptake by the vesicles occurred after one hour incubation. The maximum concentration of Ca2+ found in the vesicles was four times greater than the external Ca2+ concentration showing that the majority of the Ca2+ was membrane bound.
The Ca2+ taken up by the vesicles was probably bound to the vesicle's interior since it was not replaced by exposure of loaded vesicles to La3+ (5 mM). The uptake of Ca2+ by the vesicles at different Ca2+ concentrations was analyzed and a high affinity Ca2+ binding site was found with an association constant for Ca2+ of 5×10−5 M. More of these sites were found in the duodenum than the ileum and vitamin D increases the number of these sites.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Busse D, Jahn A, Steinmaier G (1975) Carrier-mediated transfer ofd-glucose in brush border vesicles derived from rabbit renal tubules. Na+-dependent versus Na+-independent transfer. Biochim Biophys Acta 401:231–243
Clark ML, Lanz HC, Senior JR, (1969) Enzymatic distinction of rat intestinal cell brush border and endoplasmic reticular membranes. Biochim Biophys Acta 183:233–235
Gmaj P, Murer H, Kinne R (1979) Calcium ion transport across plasma membranes isolated from rat kidney cortex. Biochem J 178:549–557
Haase W, Schafer A, Murer H, Kinne R (1978) Studies on the orientation of brush-border membrane vesicles. Biochem J 172:57–62
Hopfer U (1978) Transport in isolated plasma membranes. Am J Physiol 234:F89-F96
Hopfer U, Nelson K, Perrotto J, Isselbacher KJ (1973) Glucose transport in isolated brush-border membrane from rat small intestine. J Biol Chem 248:25–32
Hubscher G, West GR, Brindley DN (1975) Studies on the fractionation of mucosal homogenates from the small intestine. Biochem J 97:629–642
Koldovsky O (1971) Developmental changes of β-galactosidase and β-glucuronidase in the rat liver and kidney. Arch Biochem Biophys 142:378–381
Lawson DEM (1978) Biochemical responses of the intestine to vitamin D. In: Lawson DEM (ed) Vitamin D. Academic Press, London & New York, p 167
Lawson DEM, Wilson PW, Barker DC, Kodicek E (1969) The isolation of chick intestinal nuclei. Effect of vitamin D3 on nuclear metabolism. Biochem J 115:263–268
Max EE, Goodman DPB, Rasmussen H (1978) Purification and characterisation of chick intestine brush-border membrane. Effects of 1α(OH) vitamin D3 treatment. Biochim Biophys Acta 511:224–236
Norman AW (1978) Calcium and phosphorus absorption. In: Lawson DEM (ed) Vitamin D. Academic Press, London and New York, p 93
Parvin R, Smith RD (1969) Phosphoramidates v. Probable identity of rat liver microsomal glucose-6-phosphatase, phosphoramidase phosphoramidate-hexose phosphotransferase. Biochemistry 8:1748–1755
Quigley JP, Gotterer GS (1969) Distribution of (Na+−K+) stimulated ATPase activity in rat intestinal mucosa. Biochim Biophys Acta 173:456–468
Rasmussen H, Fontaine O, Max EE, Goodman DBP (1979) The effect of 1α-hydroxyvitamin D3 administration on calcium transport in chick intestine brush-border membrane vesicles. J Biol Chem 254:2993–2999
Sigrist-Nelson K, Murer H, Hopfer U (1975) Active alanine transport in isolated brush-border membranes. J Biol Chem 250:5674–5680
Strobel HW, Digham JD (1978) Purification and properties of NADPH-Cytochrome P-450 reductase. In: Fleischer S, Packer L (eds) Methods of enzymology, vol LII, part C. Academic Press, New York and London, p 89
Wasserman RH, Taylor AN (1969) Intestinal absorption of calcium. In: Comar CL, Bronner F (eds) Mineral metabolism, vol. 3. Academic Press, New York and London, p 321
Wharton DC, Tzagaloff A (1967) Cytochrome oxidase from beef heart mitochondria. In: Estabrook RW, Pullman ME (eds) Methods of enzymology, vol X. Academic Press, New York and London, p 245
Wilson PW, Lawson DEM (1977) 1,25-Dihydroxyvitamin D stimulation of specific membrane proteins in chick intestine. Biochim Biophys Acta 497:805–811
Wilson PW, Lawson DEM (1978) Incorporation of [3H]leucine into an actin-like protein in response to 1,25-dihydroxycholecalciferol in chick intestinal brush-borders. Biochem J 173:627–631
Yamagami K, Terayama H (1979) Ca2+ binding sites in plasma membranes of rat liver and hepatoma cells, and effect of concanavalin A on the Ca2+ binding sites and cellular uptake of Ca2+. Biochim Biophys Acta 558:199–213
About this article
Cite this article
Wilson, P.W., Lawson, D.E.M. Calcium binding activity by chick intestinal brush-border membrane vesicles. Pflugers Arch. 389, 69–74 (1980). https://doi.org/10.1007/BF00587930
- Vitamin D
- Intestinal vesicles
- Brush-border membranes