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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Calcium binding activity by chick intestinal brush-border membrane vesicles

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Uptake of Ca2+ by vesicle preparation of chick intestinal brush-border membranes was rapid and extensive. With tracer quantities of Ca2+ uptake was complete in 10 min whereas with 2.0 mM Ca2+ maximum uptake by the vesicles occurred after one hour incubation. The maximum concentration of Ca2+ found in the vesicles was four times greater than the external Ca2+ concentration showing that the majority of the Ca2+ was membrane bound.

The Ca2+ taken up by the vesicles was probably bound to the vesicle's interior since it was not replaced by exposure of loaded vesicles to La3+ (5 mM). The uptake of Ca2+ by the vesicles at different Ca2+ concentrations was analyzed and a high affinity Ca2+ binding site was found with an association constant for Ca2+ of 5×10−5 M. More of these sites were found in the duodenum than the ileum and vitamin D increases the number of these sites.

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Wilson, P.W., Lawson, D.E.M. Calcium binding activity by chick intestinal brush-border membrane vesicles. Pflugers Arch. 389, 69–74 (1980). https://doi.org/10.1007/BF00587930

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Key words

  • Calcium
  • Vitamin D
  • Intestinal vesicles
  • Brush-border membranes