We have cloned GAM2, which is required for transcription ofSTA1, a gene encoding an extracellular glucoamylase inSaccharomyces cerevisiae var.diastaticus. DNA sequence analysis revealed thatGAM2 is the same gene asSIN3, known to be a general negative regulator of yeast genes. RNA blot analysis indicated thatGAM2/SIN3 also acts as a positive regulator ofGAM3/ADR6, which in turn is required for transcription ofSTA1 andADH2. These results suggest thatGAM2 regulatesSTA1 expression through transcriptional activation ofGAM3 and indicate that GAM2/SIN3 protein is a transcriptional regulator that can play a role in both activation and repression of transcription.
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Yoshimoto, H., Ohmae, M. & Yamashita, I. TheSaccharomyces cerevisiae GAM2/SIN3 protein plays a role in both activation and repression of transcription. Molec. Gen. Genet. 233, 327–330 (1992). https://doi.org/10.1007/BF00587597
- Transcriptional regulator