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Isolation and characterization of cDNA clones encodingcdc2 homologues fromOryza sativa: a functional homologue and cognate variants

Summary

Using probes obtained by PCR amplification, we have isolated two cognate rice cDNAs (cdc2Os-1 andcdc2Os-2) encoding structural homologues of thecdc2 +/CDC28(cdc2) protein kinase from a cDNA library prepared from cultured rice cells. Comparison of the deduced amino acid sequences of cdc2Os-1 and cdc2Os-2 showed that they are 83 % identical. They are 62 % identical toCDC28 ofSaccharomyces cerevisiae and much more similar to the yeast and mammalian p34cdc2 kinases than to riceR2, acdc2-related kinase isolated previously by screening the same rice cDNA library with a different oligonucleotide probe. Southern blot analysis indicated that the three rice clones (cdc2Os-1,cdc2Os-2 andR2) are derived from distinct genes and are each found in a single copy per rice haploid genome. RNA blot analysis revealed that these genes are expressed in proliferating rice cells and in young rice seedlings.cdc2Os-1 could complement a temperature-sensitive yeast mutant ofcdc28. However, despite the similarity in structure, bothcdc2Os-2 andR2 were unable to complement the same mutant. Thus, the present results demonstrate the presence of structurally related, but functionally distinct cognates of thecdc2 cell cycle kinase in rice.

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Author information

Correspondence to Junji Hashimoto.

Additional information

The nucleotide sequence data in this paper have been deposited in the EMBL database under accession number X60374 (cdc2Os-1) and X60375 (cdc2Os-2)

Communicated by K. Isono

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Hashimoto, J., Hirabayashi, T., Hayano, Y. et al. Isolation and characterization of cDNA clones encodingcdc2 homologues fromOryza sativa: a functional homologue and cognate variants. Molec. Gen. Genet. 233, 10–16 (1992). https://doi.org/10.1007/BF00587555

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Key words

  • p34cdc2
  • Oryza sativa
  • Protein kinase
  • Cell cycle control