Pflügers Archiv

, Volume 341, Issue 1, pp 63–72 | Cite as

Critical evaluation of methods for determination of blood volume in the dog

  • U. Finsterer
  • P. Prucksunand
  • H. Brechtelsbauer


In 7 mongrel dogs a total of 202 measurements of blood volume were performed according to three different methods (RIHSA, Cr51, double-isotope method). Careful weighing of tracers and samples proved to be essential for correct measurements. Large vessel hematocrit values obtained by centrifugation and by the distribution of RIHSA in whole blood and in plasma were essentially equal. The disappearance rate of RIHSA averaged 10% per hour, while that of Cr51 differed significantly between dogs with and without saline infusion (20.9 and 13.7% per hour respectively). Values of blood volume for the fasting conscious dog averaged 80 ml/kg of BW with no significant difference between the three methods of determination. The correlation between blood volumes obtained by synchronized determination with Cr51 and RIHSA was rather weak (r=0.7143, standard deviation of the mean error ±14.3%). In agreement with this, the rate offcells showed a large scatter of ±7 and ±10% respectively for the two groups. It was demonstrated that the decrease of activity of Cr51 in the circulating blood due to the uptake of tagged red cells by the spleen is not linear, as initially supposed, but follows an exponential function, resulting in an error in the measurement of red cell volume of +10 and +13% respectively.

Key words

Blood Volume Cr51 Labelled Erythrocytes RIHSA Plasma Trapping Disappearance Rate fcells 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Albert, S. N.: Blood Volume, Springfield, Ill.: Ch. C. Thomas 1963.Google Scholar
  2. 2.
    Barcroft, J., Stephens, J. G.: Observations upon the size of the spleen. J. Physiol. (Lond.)64, 1 (1927).Google Scholar
  3. 3.
    Burck, H.-Chr.: Fehler und Fortschritte der Hämatokritmethode. Dtsch. med. Wschr.95, 1362 (1970).Google Scholar
  4. 4.
    Chaplin, H., Mollison, P. L.: Correction for plasma trapped in the red cell column of the hematocrit. Blood7, 1227 (1967).Google Scholar
  5. 5.
    Garby, L., Vuille, J.-C.: The amount of trapped plasma in a high speed microcapillary hematocrit centrifuge. Scand. J. clin. Lab. Invest.13, 642 (1961).Google Scholar
  6. 6.
    Hahn, P. F., Bale, W. F., Bonner, J. F.: Removal of red cells from the active circulation by sodium pentobarbital. Amer. J. Physiol.138, 415 (1942).Google Scholar
  7. 7.
    Huggins, R. A., Smith, E. L., Deavers, S., Overton, R. C.: Changes in cell and plasma volumes in the dog produced by hemorrhage and reinfusion. Amer. J. Physiol.189, 249 (1957).Google Scholar
  8. 8.
    Kramer, K., Luft, U. C.: Mobilization of red cells and oxygen from the spleen in severe hypoxia. Amer. J. Physiol.165, 215 (1951).Google Scholar
  9. 9.
    Rapaport, E., Kuida, H., Haynes, F. W., Dexter, L.: Pulmonary red cell and plasma volumes and pulmonary hematocrit in the normal dog. Amer. J. Physiol.185, 127 (1956).Google Scholar
  10. 10.
    Reeve, E. B., Gregersen, M. I., Allen, T. H., Seap, H.: Distribution of cells and plasma in the normal and splenectomized dog and its influence on blood volume estimates with P32 and T-1824. Amer. J. Physiol.175, 195 (1953).Google Scholar
  11. 11.
    Seifert, J., Jesch, F., Klövekorn, W. P., Messmer, K.: Die Blutvolumenbestimmung beim Hund im hämorrhagischen Schock. Res. exp. Med.157, 269 (1972).Google Scholar
  12. 12.
    Seifert, J., Messmer, K.: Validity of blood volume determinations in hemorrhagic shock in rats. Europ. Surg. Res.3, 306 (1971).Google Scholar
  13. 13.
    Sterling, K., Gray, S. J.: Determination of the circulating red cell volume in man by radioactive chromium. J. clin. Invest.29, 1614 (1950).Google Scholar

Copyright information

© Springer-Verlag 1973

Authors and Affiliations

  • U. Finsterer
    • 1
  • P. Prucksunand
    • 1
  • H. Brechtelsbauer
    • 1
  1. 1.Physiologisches Institut der Universität MünchenGermany

Personalised recommendations