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Measurements of facilitated diffusion of oxygen in red blood cells at 37°C

Summary

The steady state transfer of O2 across thin layers of centrifuged red cells at 37°C was measured before and after saturation of the haemoglobin with CO. The measurements were taken at 107 Torr and 116 Torr average O2 partial pressure difference, respectively.

Before CO saturation the O2 transfer was 64 percent higher than afterwards. The average difference of the O2 saturation at both surfaces of the erythrocyte layer was 82%.

The maximum of the facilitated O2 diffusion by oxyhaemoglobin diffusion was calculated to be equal to the free diffusion which occurs at 100 Torr partial pressure difference.

The rate of facilitated O2 diffusion in red cells corresponds to a diffusion coefficient of the carrier (haemoglobin) of 5–6×10−8 cm2/sec.

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References

  1. Adams, L. R., andI. Fatt: The diffusion coefficient of human hemoglobin at high concentrations. Resp. Physiol.2, 293–301 (1967).

  2. Bartels, H., K. Betke, P. Hilpert, G. Niemeyer u.K. Riegel: Die sogenannte Standard-O2-Dissoziationskurve des gesunden, erwachsenen Menschen. Pflügers Arch. ges. Physiol.272, 372–383 (1961).

  3. Collins, R. E.: Transport of gases through hemoglobin solution. Bull. Math. Biophys.23, 223–232 (1961).

  4. Fatt, I., andR. C. La Force: Theory of oxygen transport through hemoglobin solutions. Science133, 1919–1921 (1961).

  5. Grote, J., u.G. Thews: Die Bedingungen für die Sauerstoffversorgung des Herzmuskelgewebes. Pflügers Arch. ges. Physiol.276, 142–165 (1962).

  6. Hemmingsen, E.: Accelerated exchange of oxygen-18 through a membrane containing oxygen-saturated hemoglobin. Science135, 733–734 (1962).

  7. —, andP. F. Scholander: Specific transport of oxygen through hemoglobin solutions. Science132, 1379–1381 (1960).

  8. Kleihauer, E., u.K. Betke: Zur Hämoglobinbestimmung mittels Cyanhämoglobin nach Betke und Savelsberg. Ärztl. Lab.3, 202–205 (1957).

  9. Klug, A., F. Kreuzer, andF. J. W. Roughton: The diffusion of oxygen in concentrated haemoglobin solutions. Helv. physiol. pharmacol. Acta14, 121–128 (1956).

  10. Koyama, T., u.K. Brecht: Ein Beitrag zur Stabilisierung der Pt-Elektrode und ihrer Anwendung bei gleichzeitiger Bestimmung des Sauerstoffverbrauchs und der mechanischen Aktivität des Muskels. Pflügers Arch. ges. Physiol.286, 181–188 (1965).

  11. Longmuir, I. S., andF. J. W. Roughton: The diffusion coefficients of carbon monoxide and nitrogen in haemoglobin solutions. J. Physiol. (Lond.)118, 264–275 (1952).

  12. Moll, W.: The diffusion coefficient of haemoglobin. Resp. Physiol.1, 357–365 (1966).

  13. Moll, W. The influence of haemoglobin diffusion on oxygen uptake and release by red cells. Resp. Physiol. (in press).

  14. Rathschlag-Schaefer, A. M. Private communication (1968).

  15. Roughton, F. J. W.: Diffusion and simultaneous chemical reaction velocity in haemoglobin solutions and red cell suspensions. Progr. Biophys. Biophys. Chem.9, 56–104 (1959).

  16. — Kinetics of gas transport in the blood. Brit. med. Bull.19, 80–89 (1963).

  17. Scholander, P. F.: Oxygen transport through hemoglobin solutions. Science131, 585–590 (1960).

  18. Sendroy, J., R. T. Dillon, andD. D. van Slyke: Studies of gas electrolyte equilibria in blood. XIX. The solubility and physical state of uncombined oxygen in blood. J. biol. Chem.105, 597–632 (1934).

  19. Wittenberg, J. B.: Oxygen transport — a new function proposed for myoglobin. Biol. Bull.117, 402–403 (1959).

  20. — The molecular mechanism of hemoglobin facilitated oxygen diffusion. J. biol. Chem.241, 104–114 (1966).

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with the technical assistance ofD. Nienaber andH. Büklow

Supported by the Deutsche Forschungsgemeinschaft.

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Moll, W. Measurements of facilitated diffusion of oxygen in red blood cells at 37°C. Pflugers Arch. 305, 269–278 (1969). https://doi.org/10.1007/BF00587281

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Key-Words

  • Facilitated Diffusion
  • Oxygen
  • Erythrocytes
  • Haemoglobin

Schlüsselwörter

  • Erleichterte Diffusion
  • Sauerstoff
  • Erythrocyten
  • Hämoglobin