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Influence of the membrane stabilizer diphenylhydantion on potassium and sodium movements in skeletal muscle

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The membrane stabilizer diphenylhydantoin (DPH) did not alter the net content of Na, K, Ca or Mg in frog sartorius muscle freshly incubated or actively transporting Na and K following Na-loading and K-depletion. Resting influx of K from normal Ringer was significantly reduced by DPH, and this inhibition occurred in the ouabain-insensitive K-uptake. Inhibition of K-influx by DPH was overcome when [K]0 was raised to 10 mM, and Rb-influx was not sensitive to the inhibitor in 2.5 mM Rb-Ringer. Efflux of tracer K was reduced by DPH in the presence and absence of ouabain. Exchange of muscle Na was not affected under condit in which K-exchange was significantly reduced, but DPH appeared to cause increased net loss of Na from muscles washed in Na-free medium. The inhibition by DPH of resting K-exchange was not sensitive to wide variations in [Na]0 or in [Ca]0. The results suggest that the effect of DPH on frog skeletal muscle in normal ionic environment is to reduce the resting, passive component of K-exchange across the fibre membrane. This effect is discussed in relation to the membrane stabilizing actions of diphenylhydantoin.

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O'Donnell, J.M., Kovács, T. & Szábó, B. Influence of the membrane stabilizer diphenylhydantion on potassium and sodium movements in skeletal muscle. Pflugers Arch. 358, 275–288 (1975). https://doi.org/10.1007/BF00587224

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Key words

  • Skeletal Muscle
  • Electrolytes
  • Potassium
  • Sodium
  • Flux Inhibition
  • Membrane Stabilization