Adrenergic stimulation of pepsinogen release from rabbit isolated gastric glands

  • Erik Skoubo-Kristensen
  • Jan Fryklund
Article
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Summary

Isolated gastric glands from the rabbit were used for studying the effect of catecholamines on the release of pepsinogen. Isoprenaline, adrenaline and noradrenaline stimulated pepsinogen release in a dose-dependent manner with similar maximal effects, but isoprenaline was significantly more potent than the other two agonists. The effect was mediated through β-adrenoceptors, since the response was inhibited by the β-adrenoceptor antagonist, propranolol, and since the α-adrenoceptor agonist, phenylephrine, was without effect in the concentration range 0.01–10 μM.

Concentration-response curves for isoprenaline were shifted to the right in parallel by increasing doses of propranolol, and maximal response was not influenced by propranolol per se, which indicates a competitive type of antagonism. A Schild plot showed a pA2-value for propranolol of 7.70 and the slope of the regression line was 1.02. Studies with the β1-selective antagonist pafenolol and the β2-selective antagonist ICI 118.551 demonstrated that isoprenaline acted through β1-adrenoceptors. The results suggest an adrenergic component in the control of the peptic cells in rabbit gastric mucosa.

Key words

β-Adrenoceptors Catecholamines Gastric glands Pepsinogen 
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Erik Skoubo-Kristensen
    • 1
  • Jan Fryklund
    • 2
  1. 1.Department of Surgery, Aarhus AmtssygehusUniversity of AarhusAarhusDenmark
  2. 2.Department of GI-PharmacologyAB HässleMölndalSweden

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