Pflügers Archiv

, Volume 315, Issue 2, pp 110–124 | Cite as

Über die unterschiedliche Wirkung von Calcium und Noradrenalin auf den Sauerstoffverbrauch isolierter Meerschweinchenvorhöfe

  • R. Krebs
Article
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Zusammenfassung

An isolierten, elektrisch gereizten (240/min, 5 msec, 20 V) inken Meerrschweinchenvorhöfen wurde mit einer früher beschriebenen Methode (Klaus et al., 1968a), die eine gleichzeitige und fortlaufende Bestimmung des O2-Verbrauches (polarographisch) und der Kontraktionskraft (isometrisch) erlaubt, die Wirkung verschiedener Calcium-(Ca 0,9; 1,8; 3,6; 7,2 mM/l) und Noradrenalin-(NA 10−11; 10−9; 10−7; 10−5 g/ml) Konzentrationen untersucht (Tyrodelösung, 37°C).

Der Ruhe-O2-Verbrauch der mit 1 g vorbelasteten Vorhöfe betrug 0,194\(\frac{{\mu l{\text{ O}}_{\text{2}} }}{{{\text{mg TG }} \cdot {\text{ min}}}}\). Er wurde durch keine der untersuchten Ca- und NA-Konzentrationen signifikant beeinflußt.

Reizung erhöhte den O2-Verbrauch (bei 0,9 mM/l Ca) um\(0,186{\text{ }}\frac{{\mu l{\text{ O}}_{\text{2}} }}{{{\text{mg TG }} \cdot {\text{ min}}}}\). Die Kontraktionskraft stieg unter steigenden Ca- und Noradrenalin-Konzentrationen von ca.\(20{\text{ }}\frac{{\text{g}}}{{{\text{mg TG }}}}{\text{ auf ca}}{\text{. 4}}0{\text{ }}\frac{{\text{g}}}{{{\text{mg TG }}}}{\text{ an}}{\text{.}}\)

Der O2-Verbrauch wurde durch Ca konzentrationsabhängig gesteigert. Unter NA 10−11 g/ml fand sich eine statistisch signifikante (p<0,01) Senkung des O2-Verbrauches. Bei 10−9 und 10−7 g/ml NA war er nicht vom Kontrollwert unter-schieden und nahm erst bei 10−5 g/ml sehr stark zu.

Der Wirkungsgrad wurde durch Ca zwischen 0,9 und 1,8 mM/l um ca. 50% erhöht und erreichte damit sein Maximum.

Noradrenalin in Konzentrationen von 10−11 und 10−9 g/ml steigerte den Wirkungsgrad um ca. 150%. Weitere Erhöhung der NA-Konzentration bewirkte einen steilen Abfall des Wirkungsgrades, der aber auch bei 10−5 g/ml noch um ca. 25% gegenüber den Kontrollen erhöht war.

Der Vergleich der Wirkungen von Ca und Noradrenalin zeigt, daß für gleiche Kontraktionskraftsteigerung der O2-Verbrauch unter Noradrenalin immer geringer war als er unter Ca gefunden wurde. Die Befunde werden über die positiv bathmotrope Wirkung des Noradrenalin sowie über Veränderungen im Ca-Umsatz der Herzmuskelzelle und deren Einfluß auf den oxydativen Stoffwechsel erklärt.

Schlüsselwörter

Herzmuskel Sauerstoffverbrauch Kontraktionskraft Calcium Noradrenalin 

On the difference between the action of calcium and norepinephrine on the oxygen consumption of isolated guinea-pig atria

Summary

The influence of various concentrations of Ca2+ (0.9; 1.8; 3.6 and 7.2 mM/l) and norepinephrine (NE) (10−11, 10−9, 10−7, 10−5 g/ml) on the oxygen consumption and the contractile force of isolated left guinea pig atria was studied (Tyrode solution, 37°C).

The oxygen consumption of quiescent preparations was\(0.194\frac{{\mu l{\text{ O}}_{\text{2}} }}{{{\text{mg TG }} \cdot {\text{ min}}}}\) (TG = dry weigt) under standard conditions (0.9 mM Ca2+) and the variations in the Ca2+ and NE concentrations mentioned above did not significantly influence this value. Electrically stimulated (240/min; 5 msec; 20 V) preparations showed an increased oxygen consumption\(\left( {0.380\frac{{\mu l{\text{ O}}_{\text{2}} }}{{{\text{mg TG }} \cdot {\text{ min}}}}{\text{ understandard conditions}}} \right)\). This additional O2 consumption was very closely related to the mechanical activity of the guinea pig atria under the influence of different Ca2+ concentrations. The mechanical efficiency was significantly increased by increasing Ca2+ concentration in the range from 0.9 to 1.8 mM/l. Higher Ca2+ concentration did not change this parameter.

The various concentrations of NE caused positive inotropic effects of comparable magnitude as the effect of increased Ca2+ concentrations. The oxygen consumption however, was reduced at low concentrations of NE (10−11 g/ml) and unchanged at medium concentrations (10−9, 10−7 g/ml), only the highest concentration tested (10−5 g/ml) produced an augmentation of the oxygen consumption as compared to the control value. This dissociation between the effects of NE on the contractile behaviour and on the oxygen consumption resulted in markedly increased mechanical efficiencies under the influence of low concentrations of NE.

The results demonstrate that the same degree of positive inotropic change can be connected with different values of myocardial oxygen consumption, depending on the nature of the inotropic agent.

These results are interpreted on the basis of recent findings on the myocardial Ca metabolism under the influence of NE and increased extracellular Ca-concentrations.

Key-Words

Heart Muscle Oxygen Consumption Muscle Contraction Calcium Norepinephrine 
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Copyright information

© Springer-Verlag 1970

Authors and Affiliations

  • R. Krebs
    • 1
  1. 1.Pharmakologisches Institut der UniversitätMainz

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