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Distribution and circulation of extracellular fluid and protein during different states of hydration in the cat

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  1. 1.

    Determinations of plasma volume (PV) and circulating protein in 13 anaesthetized cats before and after dehydration by deprivation of water for 48 h revealed an 11.5% decrease of PV and a 13.5% decrease of circulating protein, the plasma protein concentration and the albumin/globulin ratio remaining essentially constant. Extracellular fluid volume (ECFV) was reduced by 13.8% in 9 dehydrated animals as compared to 10 control animals.

  2. 2.

    The fractional disappearance rate of radio albumin revealed that half a pool of albumin is exchanging with the extravascular space in 10 h in control animals as well as in dehydrated animals. The observation that lymph flow and lymph protein transport from the thoracic duct were closely correlated with the state of filling of the interstitial space explains why half a pool of plasma albumin was returned to the blood stream in 16.5 h in dehydrated animals as compared to 10 h in controls. The reduction of plasma albumin due to dehydration was calculated to result from the disturbance of the dynamic equilibrium between transcapillary albumin shift and lymphatic return.

  3. 3.

    During rehydration by saline infusion, dehydrated animals kept larger percentages of the infused fluid inside the intravascular space demonstrating a decrease of net transcapillary fluid shift. The increase of lymph flow during infusion was shown to be closely correlated with the filtration rate. There was an increase of transcapillary protein shift and a decrease of transport rates for lymph protein during infusion.

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  1. 1.

    Adolph, E. F.: Blood changes in dehydration. In: Physiology of man in the desert. New York-London: Hafner Publ. 1969.

  2. 2.

    Albert, S. N., Hirsch, E. F., Economopoulos, B., Albert, C. A.: Tripletracer technique for measuring red-blood-cell, plasma and extracellular-fluid volume. J. nucl. Med.9, 19 (1967).

  3. 3.

    Baratz, R. A., Ingraham, R. C.: Capillary permeability during hemorrhagic shock in the rat. Fed. Proc.13, 7 (1954).

  4. 4.

    Bass, D. E., Henschel, A.: Responses of body fluid compartments to heat and cold. Physiol. Rev.36, 128 (1956).

  5. 5.

    Birke, G., Liljedahl, S.-O., Plantin, L.-O., Rieger, Å.: Blood volume and plasma protein. I. Changes in blood volume and plasma proteins after bleeding in the splenectomized dog. Acta chir. scand.132, 477 (1966).

  6. 6.

    Caldwell, G. T.: Studies in water metabolism of the cat. The influence of dehydration on blood concentration, thermoregulation, respiratory exchange, and metabolic-water production. Physiol. Zool.4, 324 (1931).

  7. 7.

    Campbell, R. M., Cuthberson, D. P., Matthews, C. M., McFarlane, A. S.: Behaviour of14C- and131I-labelled plasma proteins in the rat. Int. J. appl. Radiat.1, 66 (1956).

  8. 8.

    Courtice, F. C.: Lymph and plasma proteins: barriers to their movement throughout the extracellular fluid. Lymphology1, 9 (1971).

  9. 9.

    D'Amato, H. E., Hegnauer, A. H.: Blood volume in the hypothermic dog. Amer. J. Physiol.173, 100 (1953).

  10. 10.

    Darrow, D. C., Yannet, H.: Metabolic studies of the changes in body electrolyte and distribution of body water induced experimentally by deficit of extracellular electrolyte. J. clin. Invest.15, 419 (1936).

  11. 11.

    Drinker, C. K.: Extravascular protein and the lymphatic system. Ann. N. Y. Acad. Sci.46, 807 (1946).

  12. 12.

    Drinker, C. K., Field, M. E.: The protein content of mammalian lymph and the relation of lymph to tissue fluid. Amer. J. Physiol.97, 32 (1931).

  13. 13.

    Dyrbye, M. O., Kragelund, E.: Simultaneous determination of the apparent3HOH,82Br, and125I human albumin and51Cr red cell volumes in human subjects. Scand. J. clin. Lab. Invest.26, 61 (1970).

  14. 14.

    Field, M. E., Drinker, C. K.: Permeability of capillaries of dog to protein. Amer. J. Physiol.97, 40 (1931).

  15. 15.

    Fine, J., Seligman, A. M.: Traumatic shock. VII. A study of the problem of the “lost plasma” in hemorrhagic, tourniquet, and burn shock by the use of radioactive Jodo-Plasma Protein. J. clin. Invest.23, 720 (1944).

  16. 16.

    Flexner, L. B., Cowie, D. B., Vosburgh, G. J.: Studies on capillary permeability with tracer substances. Cold Spr. Harb. Symp. quant. Biol.XIII, 88 (1948).

  17. 17.

    Földi, M., Rusznyák, I., Szabó, Gy.: The role of lymph-circulation in the pathogenesis of edema. Acta med. Acad. Sci. hung.3, 259 (1952).

  18. 18.

    Gamble, J. L.: Chemical anatomy, physiology and pathology of extracellular fluid. Cambridge, Mass.: Harvard University Press 1954.

  19. 19.

    Gersh, I., Catchpole, H. R.: The nature of ground substance of connective tissue. Persp. Biol. Med.3, 282 (1960).

  20. 20.

    Guyton, A. C., Granger, J. H., Taylor, A. E.: Interstitial fluid pressure. Physiol. Rev.51, 527 (1971).

  21. 21.

    Guyton, A. C., Lindley, J. E., Touchstone, R. N., Smith, Ch. M., Batson, H. M.: Effects of massive transfusion and hemorrhage on blood pressure and fluid shifts. Amer. J. Physiol.163, 529 (1950).

  22. 22.

    Guyton, A. C., Scheel, K., Murphree, D.: Interstistial fluid pressure: III. Its effect on resistance to tissue fluid mobility. Circulat. Res.19, 412 (1966).

  23. 23.

    Hatton, G. T.: Time course of blood changes during acute water deprivation in rats. Physiol. Behav.7, 35 (1971).

  24. 24.

    Hopper, J., Elkington, J. R., Winkler, A. W.: Plasma volume of dogs in dehydration with and without salt loss. J. clin. Invest.23, 110 (1944).

  25. 25.

    Horowitz, M., Borut, A.: Effect of acute dehydration on body fluid compartments in three rodent species, rattus norvegicus, acomys cahirinus and meriones crassus. Comp. Biochem. Physiol.35, 283 (1970).

  26. 26.

    Kaltreider, N. L., Meneely, G. R.: The effect of exercise on the volume of the blood. J. clin. Invest.19, 627 (1940).

  27. 27.

    Kirsch, K., Schultze, G., Lange, L., Echt, M.: Die Bedeutung der Wasserbilanz für die Homöostase des Niederdrucksystems. Verh. dtsch. Ges. Kreisl.-forsch. (in print) (1972b).

  28. 28.

    Kirsch, K., Schultze, G., Röcker, L., Bierbaum, U., Eckert, P.: The effect of exercise and dehydration on plasma volume and central venous pressure. Z. Kreisl.-Forsch. (in print) (1972a).

  29. 29.

    Korner, P. I., Morris, B., Courtice, F. C.: An analysis of factors affecting lymph flow and protein composition during gastric absorption of food and fluids, and during intravenous infusion. Aust. J. exp. Biol. med. Sci.32, 301 (1954).

  30. 30.

    Leak, L. V.: Electron microscopic observations on lymphatic capillaries and the structural components of the connective tissue-lymph interface. Microvasc. Res.2, 361 (1970).

  31. 31.

    Lee, J. S.: Analysis on diffusion and convection of protein in tissue. J. appl. Physiol.32, 254 (1972).

  32. 32.

    Liljedahl, S. O., Rieger, Å.: Importance of thoracic duct lymph in resistution of plasma volume and plasma proteins after bleeding and immediate substitution in the splenectomized dog. Acta chir. scand., Suppl.379, 39 (1967).

  33. 33.

    MacFarlance, W. V., Morris, R. J., Howard, B.: Water economy of tropical Merino sheep. Nature (Lond.)178, 304 (1956).

  34. 34.

    MacFarlane, W. V., Morris, R. J. H., Howard, B.: Turn-over and distribution of water in desert camels, sheep, cattle, and kangaroos. Nature (Lond.)197, 270 (1963).

  35. 35.

    Maloiy, G. M. O., Boarer, C. D. H.: Responses of the somali donkey to dehydration: hematological changes. Amer. J. Physiol.221, 37 (1971).

  36. 36.

    Matthews, C. M. E., Freeman, T.: The albumin pool. In: Compartments, pools, and spaces in medical physiology. Oak Ridge, Tennessee: U.S. Atomic Energy Commission 1967.

  37. 37.

    Mayerson, H. S.: The physiologic importance of lymph. Handbook of Physiology, Circulation II, p. 1035. Washington 1963.

  38. 38.

    Moore, F. D., Dagher, F. J., Boyden, C. M., Lee, C. J., Lyons, J. H.: Hemorrhage in normal man: I. Distribution and dispersal of saline infusions following acute blood loss: Clinical kinetics of blood volume support. Ann. Surg.163, 458 (1966).

  39. 39.

    Moore, F. D., Mudowney, F. P., Haxhe, J. J., Marczynska, A. W., Ball, M. R., Boyden, C. M.: Body composition in the dog. I. Findings in the normal animal. J. surg. Res.2, 245 (1962).

  40. 40.

    Nadal, J. W., Pedersen, S., Maddock, W. G.: A comparison between dehydration from salt loss and from water deprivation. J. clin. Invest.20, 691 (1941).

  41. 41.

    Oeff, K.: Umsatz von radioaktiven Serumeiweißfraktionen. II. Versuche an normalen Ratten. Z. ges. exp. Med.123, 309 (1954).

  42. 42.

    Painter, E. E., Holmes, J. H., Gregersen, M. I.: Exchange and distribution of fluid in dehydration in the dog. Amer. J. Physiol.52, 66 (1948).

  43. 43.

    Prentiss, P. G., Wolf, A. V., Eddy, H. A.: Hydropenia in cat and dog. Ability of the cat to meet its water requirements solely from a diet of fish or meat. Amer. J. Physiol.196, 625 (1959).

  44. 44.

    Reeve, E. B.: The plasma albumin system. In: Dynamic clinical studies with radioisotopes. Oak Ridge, Tennessee: U.S. Atomic Energy Commission 1964.

  45. 45.

    Reeve, E. B., Chen, Y. A.: Regulation of interstitial albumin. In: Plasma protein metabolism. Ed. A. Rothschild and Th. Waldmann. New York-London: Academic Press 1970.

  46. 46.

    Rieger, Å.: Blood volume and plasma protein. II. Changes in blood volume and plasma proteins after bleeding and immediate substitution with physiologic saline, dog plasma and dog albumin in the splenectomized dog. Acta chir. scand., Suppl.379, 3 (1967).

  47. 47.

    Rodbard, H. S., Malin, A., Young, C.: Significance of changes in plasma and extracellular volumes in induced hyperthermia and hypothermia. Amer. J. Physiol.167, 485 (1951).

  48. 48.

    Rosenblum, W. I., Asofsky, R. M.: Effects of dehydration on blood viscosity and on distribution of plasma proteins in experimental macroglobulinaemia. Nature (Lond.)216, 1327 (1967).

  49. 49.

    Rusznyák, I., Földi, m., Szabó, G.: Lymphologie, Physiologie und Pathologie der Lymphgefäße und des Lymphkreislaufes. Stuttgart: G. Fischer 1969.

  50. 50.

    Scatchard, G., Batchelder, A. C., Brown, A.: Chemical, clinical, and immunological studies on the products of human plasma fractionation. VI. The osmotic pressure of plasma and of serum albumin. J. clin. Invest.23, 458 (1944).

  51. 51.

    Schmidt-Nielsen, K.: Desert Animals. Oxford: Clarendon Press 1964.

  52. 52.

    Schultze, H. E., Heremans, J. F.: Molecular biology of human proteins. I: Nature and metabolism of extracellular proteins. Amsterdam-London-New York: Elsevier Publ. Comp. 1966.

  53. 53.

    Schultze, G., Kirsch, K., Röcker, L.: Menge und Zusammensetzung des Nettokapillarfiltrats in Abhängigkeit vom Hydrierungszustand. Symposion Gefäßwand und Blutplasma IV, Leipzig 1972 (in print).

  54. 54.

    Senay, L. C., Jr.: Movement of water, protein and crystalloids between vascular and extravascular compartments in heat-exposed men during dehydration and following limited relief of dehydration. J. Physiol. (Lond.)210, 617 (1970).

  55. 55.

    Senay, L. C., Jr., Christensen, M. L.: Changes in blood plasma during progressive dehydration. J. appl. Physiol.20, 1136 (1965).

  56. 56.

    Senay, L. C., Jr., Christensen, M. L.: Variations of certain blood constituents during acute heat exposure. J. appl. Physiol.24, 302 (1968).

  57. 57.

    Shires, T., Williams, J., Brown, F.: Simultaneous measurement of plasma volume, extracellular fluid volume, and red blood cell mass in man utilizing I131, S35O4, and Cr51. J. Lab. clin. Med.55, 776 (1960).

  58. 58.

    Siegenthaler, W.: Wasser und Elektrolythaushalt. In: Klinische Pathophysiologie. Stuttgart: G. Thieme (1970).

  59. 59.

    Simmonds, J. W.: The effect of fluid, electrolyte and food intake on thoracic duct lymph flow in unanaesthetized rats. Aust. J. exp. Biol. med. Sci.32, 285 (1954).

  60. 60.

    Snashall, P. D., Lucas, J., Guz, A., Floyer, M. A.: Measurement of interstitial fluid pressure by means of a cotton wick in man and animals and analysis of the origin of the pressure. Clin. Sci.41, 35 (1971).

  61. 61.

    Swan, R. C., Madisso, H., Pitts, R. F.: Measurement of extracellular fluid volume in nephrectomized dogs. J. clin. Invest.33, 1447 (1954).

  62. 62.

    Szabó, G., Magyar, Z., Papp, N.: Correlation between capillary filtration and lymph flow in venous congestion. Acta med. Acad. Sci. hung.19, 185 (1963).

  63. 63.

    Szabó, G., Magyar, Z., Zoltán Ö. T.: Über die Wirkung der Venenstauung und Hypoproteinämie auf den transkapillaren Eiweißaustausch. Acta med. Acad. Sci. hung.18, 219 (1962).

  64. 64.

    Walser, M., Seldin, D. W., Grollman, A.: An evaluation of radiosulfate for the determination of the volume of extracellular fluid in man and dogs. J. clin. Invest.32, 299 (1953).

  65. 65.

    Wasserman, K., Joseph, J. D., Mayerson, H. S.: Kinetics of vascular and extravascular protein exchange in unbled and bled dogs. Amer. J. Physiol.184, 175 (1956).

  66. 66.

    Wasserman, K., Mayerson, H. S.: Exchange of albumin between plasma and lymph. Amer. J. Physiol.165, 15 (1951).

  67. 67.

    Wasserman, K., Mayerson, H. S.: Mechanism of plasma protein changes following saline infusion. Amer. J. Physiol.170, 1 (1952).

  68. 68.

    Wassermann, K., Mayerson, H. S.: Plasma volume changes compared with hematocrit and plasma protein changes after hemorrhage. Amer. J. Physiol.182, 419 (1955).

  69. 69.

    Wetterfors, J.: Albumin, a clinical and experimental study. Acta med. scand.177, Suppl. 430 (1965).

  70. 70.

    White, J. C., Field, M. E., Drinker, C. K.: On the protein content and normal flow of lymph from the foot of the dog. Amer. J. Physiol.103, 34 (1933).

  71. 71.

    Wyndham, C. H., Benade, A. J. A., Williams, C. G., Strydom, N. B., Goldin, A., Heyns, A. J. A.: Changes in central circulation and body fluid spaces during acclimatization to heat. J. appl. Physiol.25, 586 (1968).

  72. 72.

    Yoffey, J. M., Courtice, F. C.: Lymphatics, lymph and lymphoid tissue. London: Edw. Arnold Ltd. 1956.

  73. 73.

    Yousef, M. K., Dill, D. B., Mayes, M. G.: Shifts in body fluids during dehydration in the burro, Equus asinus. J. appl. Physiol.29, 345 (1970).

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This study was supported by Contract No. F 44620-71-C-0117 of the Air Force Office of Scientific Research, European Office of Aerospace Research (OAR) and Deutsche Forschungsgemeinschaft.

A preliminary report was given at the 39th Congress of the German Physiological Society, Erlange, April 1972.

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Schultze, G., Kirsch, K., Röcker, L. et al. Distribution and circulation of extracellular fluid and protein during different states of hydration in the cat. Pflugers Arch. 337, 351–366 (1972). https://doi.org/10.1007/BF00586651

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Key words

  • Dehydration
  • Extracellular Fluid Space
  • Plasma Volume
  • Plasma Protein
  • Transcapillary Exchange
  • Lymphatic Transport
  • Rehydration