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A new technique for rapid freezing of tissue specimens from isolated skeletal muscles

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Summary

A new technique for rapid sampling and freezing of tissue specimens from isolated skeletal muscles is described. A sharp-edged steel tube is connected to the spring of an air gun and shot obliquely through the muscle thereby excising a tissue cylinder. Exactly at the moment when the steel tube leaves the undersurface of the muscle the excised muscle cylinder is shot directly into a cooling agent by the increased pressure developed by the modified air gun. The time between excision and immersion of the muscle cylinder in the cooling agent does not exceed 10 msec. Therefore, the rapidity of cooling is practically limited by the thermal conductivity of the cooling agents and by the isolating gas cover formed around the tissue specimens. Experiments in dogs show that up to 6 specimens can be shot from one gastrocnemius by this technique without interrupting contractions.

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Supported by the Deutsche Forschungsgemeinschaft.

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Hirche, H., Langohr, H.D., Oswald, S. et al. A new technique for rapid freezing of tissue specimens from isolated skeletal muscles. Pflugers Arch. 343, 89–94 (1973). https://doi.org/10.1007/BF00586577

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Key words

  • Rapid Freezing Technique for Muscle Tissue
  • Muscle Metabolism