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Catecholamine excretion by the healthy adult human

Summary

During the past few years much progress has been made concerning the development of accurate chemical methods for the determination of adrenaline, noradrenaline, and hydroxy-methoxy mandelic acid in urine and blood plasma. The problem of establishing standard values for the healthy average adult human of both sexes has thus become more urgent. We determined creatinine, adrenaline and noradrenaline (trihydroxyindole method) as well as hydroxy-methoxy mandelic acid (HMMA) in 16 men and 11 women. The daily excretion of creatinine remained to within 5% in 12 of the 27 subjects but varied by more than 5% to over 20% in the remaining 15 subjects. The mean values of the 24 hr urinary catecholamine excretion were: for men: noradrenaline = 24.3±6.9 μg, adrenaline = 7.1±2.9 μg, the NA/A ratio = 4.1±2.3, HMMA=6.9±2.1 mg; for women: noradrenaline = 23.1±7.1 μg, adrenaline = 5.6±2.4 μg, ratio = 5.1±3.6, HMMA=6.2±2.5 mg. A study of the diurnal variation showed that adrenaline and noradrenaline excretion was highest during the period from 7–15 hr, decreased from 15–23 hr by 20% for noradrenaline and by 30% for adrenaline, and further decreased during the time from 23–7 hr by 45% for noradrenaline and 75% for adrenaline; the excretion of HMMA was the same during the two day-time periods and was 35% lower during the night. The resting plasma levels were: for men: noradrenaline = 0.12 μg/l and adrenaline = 0.23 μg/l; for women: noradrenaline = 0.11 μg/l and adrenaline =0.21 μg/l. The values reported here agreed well with data obtained by Kärki (1956) from bioassay and were of the same order of magnitude as those collected by Brunjes (1964) using a different version of the trihydroxyidole method. The discrepancies with some other studies were critically reviewed.

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References

  1. Anton, A. H.: Catecholamines in human plasma. Science151, 709 (1966).

  2. — Gravenstein, J. S., Wheat, M. W.: Extracorporeal circulation and endogenous epinephrine and norepinephrine in plasma, atrium, and urine in man. Anesthesiology25, 262 (1964).

  3. — Sayre, D. F.: A study of the factors affecting the aluminum oxide-trihydroxyindole procedure for the analysis of catecholamines. J. Pharmacol. exp. Ther.138, 360 (1962).

  4. Bonsnes, R. W., Taussky, H. H.: On the colorimetric determination of creatinine by the Jaffe reaction. J. biol. Chem.158, 581 (1945).

  5. Brunjes, S.: Catecholamine metabolism in essential hypertension. New Engl. J. Med.27, 120 (1964).

  6. Connelian, T. P., Godfrey, J. M.: The routine determination of urinary 4-hydroxy-3-methoxy mandelic acid. Clin. chim. Acta.9, 410 (1964).

  7. Crout, J. R.: Catecholamines in urine. In: Standard Methods of Clinical Chemistry. New York: Academic Press 1961.

  8. Cunningham, W. L., Becker, E. J., Kreuzer, F.: Catecholamines in plasma and urine at high altitude. J. appl. Physiol.20, 607 (1965).

  9. Dauchy, F., Giudicelli, J. F.: Nouvelles méthodes de dosage de l'acide vanilmandélique, de la métadrénaline et de la normétadrénaline dans l'urine. Ann. Biol. clin.23, 769 (1965).

  10. Euler, U. S. v.: The catecholamines. Adrenaline; Noradrenaline. In: Hormones in blood (Eds. C. H. Gray and A. L. Bacharach), p. 515. New York: Academic Press Inc. 1961.

  11. —: Quantitation of stress by catecholamine analysis. Clin. Pharmacol. Ther.5, 398 (1964).

  12. —: Evaluation of stress by quantitative hormone studies. In: First Intern. Symp. on Basic Environm. Problems of Man in Space, Paris, 1962, p. 308. Berlin-Heidelberg-New York: Springer 1965.

  13. — Hellner-Björkman, S., Orwén, I.: Diurnal variations in the excretion of free and conjugated noradrenaline and adrenaline in urine from healthy subjects. Acta physiol. scand.33, Suppl. 118, 10 (1955).

  14. Filbert, M. G., Weller, J. M.: Distribution kinetics of intravenous epinephrine. Proc. Soc. exp. Biol. (N. Y.)101, 294 (1959).

  15. Garnier, B., Gertsch, R.: Autonome Hyperreflexie und Katecholaminausscheidung beim Paraplegiker. Schweiz. med. Wschr.94, 124 (1964).

  16. Häggendal, J.: An improved method for fluorimetric determination of small amounts of adrenaline and noradrenaline in plasma and tissues. Acta physiol. scand.59, 242 (1963).

  17. Hunter, R. B., Marschall, P. B., Oram, F. J.: Catecholamine excretion in normal persons and in cases of phaeochromocytoma. Quart. J. Med. (New Ser.)32, 225 (1963).

  18. Jacobs, S. L., Sobel, C., Henry, R. J.: Specificity of the trihydroxyindole method for determination of urinary catecholamines. J. clin. Endocr.21, 305 (1961).

  19. Kärki, N. T.: The urinary excretion of noradrenaline and adrenaline in different age groups, its diurnal variation and the effect of muscular work on it. Acta physiol. scand.39, Suppl. 132, 1 (1956).

  20. Kershbaum, A., Bellet, S., Jimenez, J., Feinberg, L. J.: Differences in effects of cigar and cigarette smoking on free fatty acid mobilization and catecholamine excretion. J. Amer. med. Ass.195, 1095 (1966).

  21. Klensch, H.: Der basale Noradrenalinspiegel im peripheren venösen Blut des Menschen. Pflügers Arch. ges. Physiol.290, 218 (1966).

  22. Olesen, K. H.: Body composition in normal adults. In: Symposia of the society for the study of human biology. Vol. VII: Human Body Composition, p. 177. Ed. J. Brožek. Oxford: Pergamon Press 1963.

  23. Oliver, G., Schäfer, E. A.: On the physiological action of extracts of the suprarenal capsules. J. Physiol. (Lond.)18, 230 (1895).

  24. Pekkarinen, A., Iisalo, E., Hakulinen, A., Linna, M., Rinne, U.: Excretion of vanilmandelic acid in normal and clinical conditions. Acta endocr. (Kbh.), Suppl.100, 140 (1965).

  25. Peyrin, L.: Dosage des catécholamines urinaires (méthode fluorimétriquue). Rev. franç. Étud. clin. biol.9, 1096 (1964).

  26. Varley, H., Gowenlock, A. H. (Eds.): The clinical chemistry of Monoamines. Amsterdam: Elsevier Publ. Co. 1963.

  27. Vestergaard, P., Leverett, R.: Constancy of urinary creatinine excretion. J. Lab. clin. Med.51, 211 (1958).

  28. Williams, C. M., Greer, M.: Homovanillic acid and vanilmandelic acid in diagnosis of neuroblastoma. J. Amer. med. Ass.183, 836 (1963).

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Becker, E.J., Kreuzer, F. Catecholamine excretion by the healthy adult human. Pflugers Arch. 316, 95–113 (1970). https://doi.org/10.1007/BF00586479

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Key-Words

  • Catecholamines
  • Catecholamine Metabolites
  • Catecholamine Excretion

Schlüsselwörter

  • Katecholamine
  • Katecholaminmetabolite
  • Katecholaminausscheidung