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Blockade by tetraethylammonium (TEA) and rubidium of potassium exchange in sartorius muscle fibers: Distribution of14C-TEA in muscle

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Tetraethylammonium (TEA) causes a blockade of42K-exchange in resting sartorius muscle by a mechanism that differs from that caused by rubidium ions. Whereas the blockade by rubidium of42K-efflux was antagonized by elevation of extracellular potassium, that caused by TEA was antagonized only partially. Rubidium-induced blockade has characteristics of competitive inhibition of42K-exchange while the TEA-induced blockade appears to be non-competitive. Moreover, TEA causes a greater blockade of42K-exchange in muscles bathed in hypertonic solutions than in muscles bathed in isotonic solutions. This finding may be related to the more rapid rate of42K-exchange in muscles bathed in hypertonic solutions. The equilibrium constant for the interaction between TEA and membrane receptors estimated during42K-efflux is approximately 20 mM; the equilibrium constant for rubidium ions is 1.4 mM. The14C-TEA space in sartorius muscle is about 2-times greater than the14C-inulin or sodium spaces but somewhat smaller than14C-urea space. The rates of efflux14C-TEA,14C-inulin and14C-urea are comparable and rapid. Thus, the muscle membrane does not appear to offer a barrier to the exchange of TEA.

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Supported by research grant NS-07540-05 and NS-09148-02 from the Institute of Neurological Sciences and Stroke, National Institutes of Health, U.S.P.H.S., Bethesda, Maryland.

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Volle, R.L., Glisson, S.N. & Henderson, E.G. Blockade by tetraethylammonium (TEA) and rubidium of potassium exchange in sartorius muscle fibers: Distribution of14C-TEA in muscle. Pflugers Arch. 333, 281–296 (1972).

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Key words

  • Tetraethylammonium
  • Rubidium
  • 42K-Exchange
  • Sartorius Muscle
  • 14C-TEA-Distribution