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Micropuncture and microperfusion study ofl-glucose secretion in rat kidney

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Summary

Studies with the free flow micropuncture technique have shown that the ratio of TF/Pl-glucose to TF/PInulin in proximal tubular fluid, in distal tubular fluid, and in more than half of the final urine samples measured was greater than one, which suggests thatl-glucose was actively secreted. Studies with the microperfusion technique confirmed this finding and showed thatl-glucose was secreted by the proximal tubules. A maximum rate of secretion was reached at a plasma concentration of 4 mM. The tubular secretion ofl-glucose was augmented by the presence of 16.6 mMd-glucose in tubular lumen and inhibited by 10−4 M phlorizin. Kinetic analysis showed that theV max values forl-glucose secretion in the absence and in the presence ofd-glucose are 5.0×10−10 and 6.3×10−10 mol×cm−2×sec−1 respectively which were very close to the value reported for theV max ford-glucose reabsorption. However, theK m forl-glucose secretion was 3.1 mM and was reduced to 1.6 mM whend-glucose was present in the perfusion fluid. TheK m ford-glucose reabsorption has been reported to be 0.6 mM (8). The results of this investigation were interpreted as being consistant with the hypothesis thatl-glucose secretion andd-glucose reabsorption share the same carrier system.

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A preliminary report was appeared in Fed. Proceeding Vol. 27, No. 2, 1968.

On sabbatical leave from Univ. Louisville, School of Medicine and supported by a NIAMD Special Fellowship (1-F3-AM-32,720-01) and a research grant of USPHS (AMO2217-10).

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Baumann, K., Huang, K.C. Micropuncture and microperfusion study ofl-glucose secretion in rat kidney. Pflugers Arch. 305, 155–166 (1969). https://doi.org/10.1007/BF00585842

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Key-Words

  • l-Glucose
  • Micropuncture and Microperfusion
  • Proximal Tubule
  • Active Secretion
  • Kinetic Study

Schlüsselwörter

  • l-Glucose
  • Mikropunktion und Mikroperfusion
  • proximaler Tubulus
  • aktive Sekretion
  • kinetische Studien