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Pflügers Archiv

, Volume 404, Issue 4, pp 374–377 | Cite as

A microscope stage temperature controller for the study of whole-cell or single-channel currents

  • Lee D. Chabala
  • Robert E. Sheridan
  • David C. Hodge
  • John N. Power
  • Michael P. Walsh
Excitable Tissues and Central Nervous Physiology Instruments and Techniques

Abstract

The construction of a microscope stage temperature controller is described that works equally well with upright or inverted microscopes. The control circuit directly regulates the bath temperature near the physiological preparation from 0.0°C to 40.0°C with a stability of ±0.1°C; it allows biophysical studies of whole-cell or single channel currents to be carried out at different temperatures without introducing additional electrical noise into the measurements. The device may also be of interest to neurobiologists who work with tissue-culture preparations.

Key words

Temperature regulation Voltage clamp Tissue culture 

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References

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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • Lee D. Chabala
    • 1
  • Robert E. Sheridan
    • 1
  • David C. Hodge
    • 1
  • John N. Power
    • 1
  • Michael P. Walsh
    • 1
  1. 1.Division of BiologyCalifornia Institute of TechnologyPasadenaUSA

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