Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Pharmacological evidence in vitro and in vivo for functional beta1 receptors in the cerebral circulation

  • 27 Accesses

  • 36 Citations


Blood flow in the caudate nucleus was measured by the thermoclearance technique in non-anesthetized rabbits, accompanied by recording of electrocorticogram, systemic blood pressure, andPa O 2 andPa CO 2 (mass spectrometry). Isoprenaline increased caudate nucleus blood flow and reduced systemic blood pressure. The beta2-receptor agonist, terbutaline, on the other hand, had only peripheral effects. The caudate nucleus flow response was selectively blocked by the beta1-receptor antagonist, practolol, whereas propranolol inhibited all effects of both agonists. Isoprenaline dilated isolated pieces of the tributary artery (middle cerebral) tested in cats, the effect being inhibited not only by propranolol but also by practolol. The results offer further evidence for the view that the beta-receptors in the cerebral vascular bed are of the beta1-type.

This is a preview of subscription content, log in to check access.


  1. 1.

    Aubineau, P.-F., Seylaz, J., Sercombe, R., Mamo, H.: Evidence for regional differences in the effect of beta-adrenergic stimulation on cerebral blood flow. Brain Res.61, 153–161 (1973)

  2. 2.

    Edvinsson, L., Nielsen, K. C., Owman, Ch.: Influence of initial tension and changes in sensitivity during amine-induced contractions of pial arteries in vitro. Arch. int. Pharmacodyn.208, 235–242 (1974)

  3. 3.

    Edvinsson, L., Owman, Ch.: Pharmacological characterization of adrenergic alpha and beta receptors mediating the vasomotor responses of cerebral arteries in vitro. Circulat. Res.35, 835–849 (1974)

  4. 4.

    Furchgott, R. F.: The classification of adrenoceptors (adrenergic receptors). An evaluation from the stand-point of receptor theory. In: Handbook of Experimental Pharmacology vol. 33 (H. Blaschko and E. Muscholl, eds.), pp. 283–335. Berlin-Heidelberg-New York: Springer 1972

  5. 5.

    Lowe, R. F., Gilboe, D. D.: Demonstration of alpha and beta adrenergic receptors in canine cerebral vasculature. Stroke2, 193–200 (1971)

  6. 6.

    Oberdörster, G., Lang, R., Zimmer, R.: Direct effects of α-and β-sympathomimetic amines on the cerebral circulation of the dog. Pflügers Arch.340, 145–160 (1973)

  7. 7.

    Persson, H., Olsson, T.: Some pharmacological properties of terbutaline (INN), 1-(3,5-dihydroxyphenyl)-2-(T-butylamino)-ethanol. A new sympathomimetic beta-receptor-stimulating agent. Acta med. scand. Suppl.512, 11–19 (1970)

  8. 8.

    Samorajski, T., Marks, B. H.: Localization of tritiated norepinephrine in mouse brain. J. Histochem. Cytochem.10, 392–399 (1962)

  9. 9.

    Sercombe, R., Aubineau, P.-F., Edvinsson, L., Mamo, H., Owman, Ch., Pinard, E., Seylaz, J.: Neurogenic influence on local cerebral blood flow. Effect of catecholamines or sympathetic stimulation as correlated with the sympathetic innervation. Neurology (Minneap.)25, 954–963 (1975)

  10. 10.

    Seylaz, J., Aubineau, P., Correze, J. L., Mamo, H.: Techniques for continuous measurement of local cerebral blood flow,Pa O 2,Pa CO 2, and blood pressure in the non-anesthetised animal. Pflügers Arch.340, 175–180 (1973)

  11. 11.

    Seylaz, J., Pinard, E., Correze, J. L., Aubineau, P.-F., Mamo, H.: Quantitative continuous measurement of blood gas tensions by mass spectrometry. J. appl. Physiol.37, 937–941 (1974)

  12. 12.

    Wahl, M., Kuschinsky, W., Bosse, O., Neiss, A.: Micropuncture evaluation of β-receptors in pial arteries of cats. Pflügers Arch.348, 293–303 (1974)

  13. 13.

    Weil-Malherbe, H., Whitby, L. G., Axelrod, J.: The blood-brain barrier for catecholamines in different regions of the brain. In: Regional neurochemistry (S. S. Kety and J. Elkes, eds.), pp. 284–292. Oxford-London-New York-Paris: Pergamon Press 1961

  14. 14.

    Wilson, C. W. M., Murray, A. W., Titus, E.: The effects of reserpine on uptake of epinephrine in brain and certain areas outside the blood-brain barrier. J. Pharmacol. exp. Ther.135, 11–16 (1962)

  15. 15.

    Xanalatos, C., James, I. M.: Effect of arterial CO2 pressure on the response of cerebral and hind-limb blood flow and metabolism to isoprenaline infusion in the dog. Clin. Sci.42, 63–68 (1972)

Download references

Author information

Correspondence to L. Edvinsson.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Sercombe, R., Aubineau, P., Edvinsson, L. et al. Pharmacological evidence in vitro and in vivo for functional beta1 receptors in the cerebral circulation. Pflugers Arch. 368, 241–244 (1977). https://doi.org/10.1007/BF00585202

Download citation

Key words

  • Regional CBF
  • Thermoclearance
  • In vitro pharmacology
  • Isolated brain vessels
  • Isoprenaline
  • Terbutaline
  • Practolol
  • Propranolol