Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

Renal urate transport during variations in urate synthesis in the rat

  • 21 Accesses

  • 3 Citations

Summary

In order to determine the effect of intrarenal synthesis of urate upon the urinary urate excretion in the rat, we effected large changes in urate synthesis by increasing it with hypoxanthine and decreasing it with allopurinol. Hypoxanthine infusion increased plasma urate rapidly and also increased the urinary urate excretion and its renal clearance. However, when the plasma urate was maintained constant, hypoxanthine had no effect upon renal urate transport. Conversely, allopurinol infusion rapidly diminished the plasma urate, urinary urate excretion and its renal clearance. Again, the maintenance of a constant plasma urate concentration prevented any change in urate transport during allopurinol. The urinary degradative purine metabolite pattern was altered pre-dictably by hypoxanthine and allopurinol. Assuming that any putative intrarenal component of urate synthesis would be affected predictably and consistently by hypoxanthine and allopurinol, these results suggest that changes in intrarenal urate synthesis are not an important determinant of urate excretion in the rat.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Abramson, R. G., Levitt, M. F.: Micropuncture study of uric acid transport in rat kidney. Amer. J. Physiol.228, 1597–1605 (1975)

  2. 2.

    Boudry, J. F.: Mechanismes de l'excretion d'acide urique chez le rat. Pflügers Arch.328, 265–278 (1971)

  3. 3.

    Cook, M. A., Adkinson, J. T.: Uric acid excretion by the rat kidney. Amer. J. Physiol.229, 586–591 (1975)

  4. 4.

    De Rougemont, D., Roch-Ramel, F.: Renal uric acid excretion studied by free-flow micropuncture in the rat. Kidney Int.5, 312 (abstract) (1974)

  5. 5.

    Greger, R., Lang, F., Deetjen, P.: Handling of uric acid by the rat kidney. I. Microanalysis of uric acid in proximal tubular fluid. Pflügers Arch.324, 279–287 (1971)

  6. 6.

    Greger, R., Lang, F., Deetjen, P.: Urate handling by the rat kidney. IV. Reabsorption in the loops of Henle. Pflügers Arch.352, 115–120 (1974)

  7. 7.

    Klinenberg, J. R., Goldfinger, S., Bradley, K. H., Seegmiller, J. B.: An enzymatic spectrophotometric method for the determination of xanthine and hypoxanthine. Clin. Chem.13, 834–846 (1967)

  8. 8.

    Kramp, R. A., Lassiter, W. E., Gottschalk, C. W.: Urate-2-14C transport in the rat nephron. J. clin. Invest.50, 35–47 (1971)

  9. 9.

    Kramp, R. A., Lenoir, R.: Characteristics of urate influx in the rat nephron. Amer. J. Physiol.229, 1654–1661 (1975)

  10. 10.

    Kramp, R. A., Lenoir, R.: Distal permeability to urate and effects of benzofuran derivatives in the rat kidney. Amer. J. Physiol.228, 875–883 (1975)

  11. 11.

    Lang, F., Greger, R., Deetjen, P.: Handling of uric acid by the rat kidney. II. Microperfusion studies on bidirectional transport of uric acid in the proximal tubule. Pflügers Arch.335, 257–265 (1972)

  12. 12.

    Lang, F., Greger, R., Deetjen, P.: Handling of uric acid by the rat kidney. III. Microperfusion studies on steady state concentration of uric acid in the proximal tubule. Pflügers Arch.338, 295–302 (1973)

  13. 13.

    Lang, F., Greger, R., Deetjen, P.: In vivo studies on uricase activity in the rat. Pflügers Arch.351, 323–330 (1974)

  14. 14.

    Liddle, L., Seegmiller, J. E., Laster, L.: The enzymatic spectrophotometric method for determination of uric acid. J. Lab. clin. Med.54, 903–913 (1959)

  15. 15.

    Oelert, H., Baumann, K., Gekle, D.: Permeabilitätsmessungen einiger schwacher organischer Säuren aus dem distalen Konvolut der Ratteniere. Pflügers Arch.307, 178–189 (1969)

  16. 16.

    Pentz, E. I.: Adaptation of the Rimini-Schryver reaction for the measurement of allantoin in urine to the Auto-Analyzer. Analyt. Biochem.27, 333–342 (1969)

  17. 17.

    Quebbemann, A. J.: Renal synthesis of uric acid. Amer. J. Physiol.224, 1398–1402 (1973)

  18. 18.

    Quebbemann, A. J., Cumming, J. D., Shideman, J. R., Toledo-Pereyra, L.: Synthesis of uric acid in isolated normothermic perfused mongrel and Dalmatian dog kidneys. Amer. J. Physiol.228, 959–963 (1975)

  19. 19.

    Quebbemann, A. J., Harvey, R. B., Cacini, W., Zmuda, M. J.: Renal formation and excretion of uric acid in the isolated perfused rat kidney. Pharmacologist15, 186 (abstract) (1974)

  20. 20.

    Roch-Ramel, F., Tellier, M., Peters, G.: Synthesis and degradation of uric acid in rat renal cortical tissue. Experientia (Basel)29, 765 (abstract) (1973)

  21. 21.

    Roch-Ramel, F., Tellier, M., Peters, G.: The role of the rat kidney in uric acid exchanges. Kidney Int.5, 312 (abstract) (1974)

  22. 22.

    Steel, R. G. D., Torrie, J. H.: Principles and Procedures of statistics, p. 67. New York: McGraw-Hill 1960

  23. 23.

    Steele, T. H.: A modified semi-automated resorcinol method for the determination of inulin. Clin. Chem.15, 1072–1078 (1969)

  24. 24.

    Weiner, I. M., Fanelli, G. M., Jr.: Renal urate excretion in animal models. Nephron14, 33–47 (1975)

  25. 25.

    Weinman, E. J., Eknoyan, G., Suki, W. N.: The influence of the extracellular fluid volume on the tubular reabsorption of uric acid. J. clin. Invest.55, 283–291 (1975)

  26. 26.

    Wyngaarden, J. B., Kelley, W. N.: Gout. In: The Metabolic basis of inherited disease (J. B. Stanbury, J. B. Wyngaarden, and D. S. Fredrickson, eds.), 3rd edition, pp. 889–968: New York: McGraw-Hill 1972

Download references

Author information

Additional information

Reported in abstract form in Clinical Research23, 508 A (1975)

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Steele, T.H., Underwood, J.L. Renal urate transport during variations in urate synthesis in the rat. Pflugers Arch. 367, 183–188 (1976). https://doi.org/10.1007/BF00585156

Download citation

Key words

  • Urate
  • Uric acid
  • Allantoin
  • Oxypurines
  • Allopurinol