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Pflügers Archiv

, Volume 367, Issue 2, pp 137–142 | Cite as

The time course of phosphorylcreatine resynthesis during recovery of the quadriceps muscle in man

  • R. C. Harris
  • R. H. T. Edwards
  • E. Hultman
  • L. O. Nordesjö
  • B. Nylind
  • K. Sahlin
Article

Summary

The time course of phosphorylcreatine (PC) resynthesis in the human m. quadriceps femoris was studied during recovery from exhaustive dynamic exercise and from isometric contraction sustained to fatigue. The immediate postexercise muscle PC content after either form of exercise was 15–16% of the resting muscle content. The time course of PC resynthesis during recovery was biphasic exhibiting a fast and a slow recovery component. The half-time for the fast component was 21–22 s but this accounted for a smaller fraction of the total PC restored during recovery from the isometric contraction than after the dynamic exercise. The half-time for the slow component was in each case more than 170 s. After 2 and 4 min recovery the total amounts of PC resynthesized after the isometric exercise were significantly lower than from the dynamic exercise.

Occlusion of the circulation to the quadriceps completely abolished the resynthesis of PC. Restoration of resynthesis occurred only after release of occlusion.

Key words

Man Muscle Exercise Recovery Phosphorylcreatine Phosphagen 

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References

  1. 1.
    Ahlborg, B., Bergström, J., Ekelund, L.-G., Guarnieri, G., Harris, R. C., Hultman, E., Nordesjö, L.-O.: Muscle metabolism during isometric exercise performed at constant force. J. appl. Physiol.33, 224–228 (1972)Google Scholar
  2. 2.
    Arese, P., Kirsten, R., Kirsten, E.: Metabolitgehalte und-Gleichgewichte nach tetanischer Kontraktion des Taubenbrustmuskels und des Rattenskelettmuskels. Biochem. Z.341, 523–533 (1965)Google Scholar
  3. 3.
    Bergström, J.: Muscle electrolytes in man. Determination by neutron activation analysis on needle biopsy specimens. A study on normal subjects, kidney patients and patients with chronic diarrhoea. Scand. J. clin. Lab. Invest.14, Suppl. 68 (1962)Google Scholar
  4. 4.
    Bergström, J., Harris, R. C., Hultman, E., Nordesjö, L.-O.: Energy rich phosphagens in dynamic and static work. In: Muscle metabolism during exercise (B. Pernow and B. Saltin, eds.), pp. 341–355 New York: Plenum Press 1971Google Scholar
  5. 5.
    Bonde-Petersen, E., Knuttgen, H. G., Henriksson, J.: Muscle metabolism during exercise with concentric and eccentric contractions. J. appl. Physiol.33, 792–795 (1972)Google Scholar
  6. 6.
    Cerretelli, P., Ambrosoli, G., Fumagalli, M.: Anaerobic recovery in man. Europ. J. appl. Physiol.34, 141–148 (1975)Google Scholar
  7. 7.
    Clarke, D. H.: Strength recovery from static and dynamic muscular fatigue. Res. Quart.33, 349–355 (1962)Google Scholar
  8. 8.
    Clarke, D. H., Stull, G. A.: Strength recovery patterns following isometric and isotonic exercise. J. Motor Behav.1, 233–243 (1969)Google Scholar
  9. 9.
    Di Prampero, P. E., Margaria, R.: Mechanical efficiency of phosphagen (ATP+CP) splitting and its speed of resynthesis. Pflügers Arch.308, 197–202 (1969)Google Scholar
  10. 10.
    Edwards, R. H. T., Harris, R. C., Hultman, E., Nordesjö, L.-O.: Phosphagen utilization and resynthesis in succesive isometric contractions, sustained to fatigue, of the quadriceps muscle in man. J. Physiol. (Lond.)224, 40–41 P (1972)Google Scholar
  11. 11.
    Edwards, R. H. T., Nordesjö, L.-O., Koh, D., Harris, R. C., Hultman, E.: Isometric exercise-factors influencing endurance and fatigue. In: Adv. Exptl. Med. Biol., Vol. 11 (B. Pernow and B. Saltin, eds.), pp. 357–360. New York: Plenum Press 1971Google Scholar
  12. 12.
    Edwards, R. H. T., Harris, R. C., Hultman, E., Kaijser, L., Koh, D., Nordesjö, L.-O.: Effect of temperature on muscle energy metabolism and endurance during succesive isometric contractions, sustained to fatigue, of the quadriceps muscle in man. J. Physiol. (Lond.)220, 335–352 (1972)Google Scholar
  13. 13.
    Funderburk, C. F., Hipskind, S. G., Welton, R. C., Lind, A. R.: Development of and recovery from fatigue induced by static effort at various tensions. J. appl. Physiol.37, 392–396 (1974)Google Scholar
  14. 14.
    Harris, R. C., Hultman, E., Nordesjö, L.-O.: Glycogen, glycolytic intermediates and high-energy phosphates determined in biopsy samples of musculus quadriceps femoris of man at rest. Methods and variance of values. Scand. J. clin. Lab. Invest.33, 109–120 (1974)Google Scholar
  15. 15.
    Harris, R. C., Hultman, E., Kaijser, L., Nordesjö, L.-O.: The effect of circulatory occlusion on isometric exercise capacity and energy metabolism on the quadriceps muscle in man. Scand. J. clin. Lab. Invest.35, 87–95 (1975)Google Scholar
  16. 16.
    Harris, R. C., Sahlin, K., Hultman, E.: On the adenine nucleotide and phosphorylcreatine content of biopsy samples of the m. quadriceps femoris of man taken after exercise. J. appl. Physiol., submitted for publicationGoogle Scholar
  17. 17.
    Hermansen, L., Osnes, J.-B.: Blood and muscle pH after maximal exercise in man. J. appl. Physiol.32, 304–308 (1972)Google Scholar
  18. 18.
    Hultman, E., Bergström, J., McLennan-Anderson, N.: Breakdown and resynthesis of phosphorylcreatine and adenosine triphosphate in connection with muscular work in man. Scand. J. clin. Lab. Invest.19, 56–66 (1967)Google Scholar
  19. 19.
    Karlsson, J., Bonde-Petersen, F., Henriksson, J., Knuttgen, H. G.: Effects of previous exercise with arms or legs on metabolism and performance in exhaustive exercise. J. appl. Physiol.38, 763–767 (1975)Google Scholar
  20. 20.
    Karlsson, J., Funderburk, C. F., Essen, B., Lind, A.: Constituents of human musle in isometric fatigue. J. appl. Physiol.38, 208–211 (1975)Google Scholar
  21. 21.
    Karlsson, J., Ollander, B.: Muscle metabolites with exhaustive static exercise of different duration. Acta physiol. scand.86, 309–314 (1972)Google Scholar
  22. 22.
    Karlsson, J., Saltin, B.: ATP and CP in working muscles during exhaustive exercise in man. J. appl. Physiol.29, 598–602 (1970)Google Scholar
  23. 23.
    Karlsson, J., Saltin, B.: Oxygen deficit and muscle metabolites in intermittent exercise. Acta physiol. scand.82, 115–122 (1971)Google Scholar
  24. 24.
    Kuby, S., Noda, L., Lardy, H. A.: Adenosinetriphosphate-creatine transphosphorylase. III. Kinetic studies. J. biol. Chem.210, 65–82 (1954)Google Scholar
  25. 25.
    Margaria, R., Edwards, H. T., Dill, D. B.: The possible mechanism of contracting and paying the oxygen debt and the role of lactic acid in muscular contraction. Amer. J. Physiol.106, 689–715 (1933)Google Scholar
  26. 26.
    Merton, P. A.: Voluntary strength and fatigue. J. Physiol. (Lond.)123, 553–564 (1954)Google Scholar
  27. 27.
    Piiper, J., Spiller, P.: Repayment of O2 debt and resynthesis of high-energy phosphates in gastrocnemius muscle of the dog. J. appl. Physiol.28, 657–662 (1970)Google Scholar
  28. 28.
    Piiper, J., Di Prampero, P. E., Cerretelli, P.: Oxygen debt and high-energy phosphates in gastrocnemius muscle of the dog. Amer. J. Physiol.215, 523–531 (1968)Google Scholar
  29. 29.
    Sacks, J.: Recovery from muscular activity and its bearing on the chemistry of contraction. Amer. J. Physiol.122, 215–223 (1938)Google Scholar
  30. 30.
    Sacks, J., Sacks, W. C.: The resynthesis of phosphocreatine after muscular contraction. Amer. J. Physiol.112, 116–123 (1935)Google Scholar
  31. 31.
    Sahlin, K., Harris, R. C., Hultman, E.: Creatine kinase equilibrium and lactate content compared with muscle pH in tissue samples obtained after isometric exercise. Biochem. J.152, 173–180 (1975)Google Scholar
  32. 32.
    Sahlin, K., Harris, R. C., Hultman, E., Nylind, B.: Lactate content and pH in muscle samples obtained after dynamic exercise. Pflügers Arch.367, 143–149 (1976)Google Scholar
  33. 33.
    Saltin, B.: Metabolic fundamentals in exercise. Med. Sci. Sports5, 137–146 (1973)Google Scholar
  34. 34.
    Saltin, B., Essen, B.: Muscle glycogen, lactate, ATP and CP in intermittent exercise. In: Muscle metabolism during exercise (B. Pernow and B. Saltin, eds.), pp. 419–424. New York: Plenum Press 1971Google Scholar
  35. 35.
    Spande, J. I., Schottelius, B. A.: Chemical basis of fatigue in isolated mouse soleus muscle. Amer. J. Physiol.219, 162–171 (1970)Google Scholar
  36. 36.
    Spronck, A. C.: In: Symposia biologica Hungarica (E. Ernst and F. Straub, eds.), Vol. 8, pp. 181–185 (1968)Google Scholar
  37. 37.
    Stull, G. A., Clarke, D. H.: Patterns of recovery following isometric and isotonic strength decrement. Med. Sci. Sports3, 135–139 (1971)Google Scholar
  38. 38.
    Tornvall, G.: Assessment of physical capabilities with special reference to the evaluation of maximal voluntary isometric muscle strength and maximal working capacity. Acta physiol. scand.58, Suppl. 201 (1963)Google Scholar
  39. 39.
    Wilson, G., Karlsson, J.: The acute effects of electrical stimulation on metabolites, enzymes and fiber recruitment pattern in human skeletal muscle. Acta physiol. scand.91, 20A (1974)Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • R. C. Harris
    • 1
  • R. H. T. Edwards
    • 2
  • E. Hultman
    • 3
  • L. O. Nordesjö
    • 4
  • B. Nylind
    • 5
  • K. Sahlin
    • 1
  1. 1.Department of Rheumatological and Metabolic ResearchS:t Eriks sjukhusStockholmSweden
  2. 2.Department of Medicine, Royal Postgraduate Medical SchoolHammersmith HospitalLondonUK
  3. 3.Department of Clinical ChemistrySerafimerlasarettetStockholmSweden
  4. 4.Department of Clinical PhysiologyUppsala Akademiska sjukhusUppsalaSweden
  5. 5.Military Medical Examination CenterKarolinska sjukhusetStockholmSweden

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