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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Effect of luminal potassium on cellular sodium activity in the early distal tubule ofAmphiuma kidney

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From previous studies it is known that a furosemide-sensitive sodium chloride cotransport system is operative in the luminal cell membrane of the early distal amphibian tubule. Since inhibition of sodium chloride cotransport prevents potassium reabsorption in this nephron segment, experiments were carried out to evaluate further the possible relationship between sodium chloride and potassium transport by studying the changes of cellular sodium activity following luminal deletion of potassium ions.

Sodium-sensitive liquid ion exchange microelectrodes and conventional microelectrodes were employed to determine the transpithelial potential (PDte), the peritubular cell membrane potential (PDpt) and the intracellular sodium activity (Nai +) in the presence and absence of luminal potassium. The ratio of the luminal cell membrane resistance over the peritubular cell membrane resistance (Rlu/Rpt) was also estimated. When potassium ions are omitted from the luminal perfusate, PDpt hyperpolarizes by some 20 mV, PDte approaches zero and Nai + decreases by about 40%. Rlu/Rpt is more than doubled in the presence of a potassium-free perfusate. Both potential and resistance changes are fully reversible. Similar results were obtained in experiments in which Barium ions (1 mmol/l BaCl2) were present during the luminal potassium substitution. Our results indicate that absence of potassium inhibits luminal sodium chloride entry; as a result of continued peritubular sodium extrusion cellular sodium activity falls. The increase of Rlu/Rpt following perfusion with a potassium-free perfusate is interpreted as a decrease of a significant electrodiffusive potassium conductance in the luminal cell membrane.

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  1. 1.

    Aiton JF, Chipperfield AR, Lamb JR, Ogden P, Simmons NL (1981) Occurrence of passive furosemide-sensitive transmembrane potassium transport in cultured cells. Biochim Biophys Acta 646:389–398

  2. 2.

    Biagi B, Kubota T, Sohtell M, Giebisch G (1981) Intracellular potentials in rabbit proximal tubules perfused in vitro. Am J Physiol 240:F200-F210

  3. 3.

    Biagi B, Sohtell M, Giebisch G (1981) Intracellular potassium activity in the rabbit proximal straight tubule. Am J Physiol 241:F677-F686

  4. 4.

    Boulpaep EL, Giebisch G (1978) Electrophysiological measurements on the renal tubule. In: Martinez-Maldonado M (ed) Methods in pharmacol, vol 4B. Plenum Publishing Corporation, New York London, pp 165–193

  5. 5.

    Burg MB, Green N (1973) Function of the thick ascending limb of Henle's loop. Am J Physiol 224:659–668

  6. 6.

    Burg M, Stoner L, Cardinal J, Green N (1973) Furosemide effect on isolated perfused tubules. Am J Physiol 225:119–124

  7. 7.

    Chipperfield AR (1981) Chloride dependence of furosemide- and Phloretin-sensitive passive sodium and potassium fluxes in human red cells. J Physiol 312:435–444

  8. 8.

    Clothier RH, Horley RTS, Balls M (1978) The structure and ultrastructure of the renal tubule of the urodele amphibian,Amphiuma means. J Anat 127:491–504

  9. 9.

    Dirks JH, Seely JF (1970) Effect of saline infusions and furosemide on the dog distal nephron. Am J Physiol 219:114–121

  10. 10.

    Geck P, Heinz E (1980) Coupling of ion flows in cell suspension systems. In: Annals of the New York Academy of Sciences, vol 341, pp 57–66

  11. 11.

    Geck P, Pietrzyk C, Burckhardt BC, Pfeiffer P, Heinz E (1980) Electrically silent cotransport of Na+, K+ and Cl in Ehrlich cells. Biochim Biophys Acta 600:432–447

  12. 12.

    Greger R (1981) Chloride reabsorption in the rabbit cortical thick ascending limb of the loop of Henle a sodium dependent process. Pflügers Arch 390:38–43

  13. 13.

    Greger R, Schlatter E (1981) Coupled transport of 2 Cl, 1 Na+ and 1 K+ at the luminal membrane of the rabbit cortical thick ascending limb of Henle's loop (ctal). 14th Annual Meeting of the American Society of Nephrology, Washington, p 147A

  14. 14.

    Greger R, Schlatter E (1982) Presence of luminal K+, a prerequisite for active NaCl transport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch 392:92–94

  15. 15.

    Greger R, schlatter E (1983) Properties of the lumen membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney Pflügers Arch (in press)

  16. 16.

    Greger R, Schlatter E (1983) Properties of the basolateral membrane of the cortical thick ascending limb of Henle's loop of rabbit kidney. A model for secondary active chloride transport. Pflügers Arch (in press)

  17. 17.

    Greger R, Schlatter E, Lang F (1983) Evidence for electroneutral chloride cotransport in the cortical thick ascending limb of Henle's loop of rabbit kidney. Pflügers Arch (in press)

  18. 18.

    Guggino WB, Stanton BA, Giebisch G (1982) Regulation of apical potassium conductance in the isolated early distal tubule of theAmphiuma kidney. Biophysical J 37:338

  19. 19.

    Imai M (1977) Effect of bumetanide and furosemide on the thick ascending limb of Henle's loop of rabbits and rats perfused in vitro Eur J Pharmacol 41:409–416

  20. 20.

    Kahn T, Goldstein MH, Alfago E, Levitt MF (1972) K+ transport and its relation to Na+ transport in distal tubule of the hydrated dog. Am J Physiol 221:1456–1463

  21. 21.

    Koenig B, Kinne R (1982) Sodium transport by plasma membranes isolated from cells of the thick ascending limb of Henle's loop. Fed Proc 41:1007

  22. 22.

    Koeppen BM, Biagi BA, Giebisch G (1982) Microelectrode characterization of the rabbit cortical collecting tubule. Am J Physiol (in press)

  23. 23.

    Matsumura T, Guggino W, Giebisch G (1982) Electrical effects of potassium and bicarbonate on proximal tubule cells of Necturus: Intracellular K affects K conductance. Kidney Int 21:281

  24. 24.

    McManus TJ, Schmidt WF III (1978) Ion and co-ion transport in avian red cells. In: Hoffman JF (ed) Membrane transport processes, vol 1 Raven Press, New York, pp 97–106

  25. 25.

    Morgan T, Tadokoro M, Marin D, Berliner RW (1970) Effect of furosemide on Na+ and K+ transport studied by microperfusion of the rat nephron. Am J Physiol 218:292–297

  26. 26.

    Oberleithner H, Giebisch G (1981) Mechanism of potassium transport across distal tubular epithelium ofAmphiuma. In: MacKnight ADC, Leader JP (eds) Epithelial ion and water transport Raven Press. New York, pp 97–105

  27. 27.

    Oberleithner H, Giebisch G (1981) Effects of furosemide and low chloride on potassium (K) transport acrossAmphiuma distal tubule-linkage of potassium (K) with chloride (Cl) reabsorption. 8th Int Congr of Nephrol, Athens 7–12 June, Abstr TT-079

  28. 28.

    Oberleithner H, Guggino W, Giebisch G (1982) Mechanism of distal tubular chloride transport inAmphiuma kidney. Am J Physiol 242:F331-F339

  29. 29.

    Oberleithner H, Lang F, Wang W, Giebisch G (1982) Effects of inhibition of chloride transport on intracellular sodium activity in distal amphibian nephron. Pflügers Arch 394:55–60

  30. 30.

    Oberleithner H, Guggino W, Giebisch G (1983) The effect of furosemide on luminal sodium, chloride and potassium transport in the early distal tubule ofAmphiuma kidney. Pflügers Arch 396:27–33

  31. 31.

    Palfrey HC, Feit PW, Greengard P (1980) cAMP-stimulated cation contransport in avian erythrocytes: Inhibition by “loop” diuretics. Am J Physiol 238:C139-C148

  32. 32.

    Robinson RA, Stokes RH (1970) Electrolyte solutions, 2nd ed. Butterworth, London

  33. 33.

    Rocha AS, Kokko JP (1973) Sodium chloride and water transport in the medullary thick ascending limb of Henle. J Clin Invest 52:612–623

  34. 34.

    Stanton B, Guggino W, Giebisch G (1981) Electrophysiology of isolated and perfused distal tubules ofAmphiuma. 14th Annual Meeting of the American Society of Nephrology, Washington, p 162A

  35. 35.

    Steiner RA, Oehme M, Ammann D, Simon W (1979) Neutral carrier sodium ion-selective microelectrode for intracellular studies. Analyt Chem 51:351–353

  36. 36.

    Stoner LC (1977) Isolated perfused amphibian renal tubules: The diluting segment. Am J Physiol 233:F438-F444

  37. 37.

    Sullivan LP, Welling DJ, Rome LA (1981) Effects of sodium and chloride on potassium transport by the bullfrog kidney. Am J Physiol 240:F127-F137

  38. 38.

    Thomas RC (1972) Intracellular sodium activity and the sodium pump in snail neurons. J Physiol 220:55–71

  39. 39.

    Velázquez H, Wright FS, Good DW (1982) Luminal influences on potassium secretion: Chloride replacement with sulfate. Am J Physiol 242:F46-F55

  40. 40.

    Walker JL (1971) Ionic specific liquid ion-exchanger microelectrodes. Anal Chem 43:89A-93A

  41. 41.

    Wiederholt M, Sullivan WJ, Giebisch G (1971) Potassium and sodium transport across single distal tubules ofAmphiuma. J Gen Physiol 57:495–525

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Author information

Correspondence to H. Oberleithner.

Additional information

This work was supported by Österr. Forschungsrat, Proj. No.: 4366 and by NIH Grant PHS AM 17433

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Oberleithner, H., Lang, F., Greger, R. et al. Effect of luminal potassium on cellular sodium activity in the early distal tubule ofAmphiuma kidney. Pflugers Arch. 396, 34–40 (1983). https://doi.org/10.1007/BF00584695

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Key words

  • Intracellular sodium activity
  • Early distal tubule
  • Sodium chloride cotransport
  • Potassium
  • Sodium sensitive microelectrode