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The control mechanism of relaxation in molluscan catch-muscle (ABRM)

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Summary

  1. 1.

    The relaxing effects of 5-Hydroxtryptamine (5-HT) have been tested on glycerol-extracted and chemically treated fibres (EDTA and Triton X-100 solutions) of ABRM in a low Ca2+ solution (10−9 M) on a high Ca2+ solution (10−6 M): at two ionic strengths (0.06 and 0.28) and two pH's (6.5 and 7.0). The resistance to stretch of the two muscle preparations has been studied in the low Ca2+ medium i.e. without contractile activity in presence and in absence of 5-HT. The presence of 5-HT reduces significantly the resistance to stretch of chemically treated fibres in conditions of ionic strength 0.28 and pH 7.0, but has no effect on glycerol extracted fibres.

  2. 2.

    After the maximal tension has been developed in a high Ca2+ solution (10−6 M) the rate of relaxation of chemically treated fibres, induced by a low Ca2+ solution (10−9M) at ionic strength 0.28 and pH 7.0 is increased by the addition of 5-HT. No effect is observed in glycerol extracted fibres.

  3. 3.

    Chemically treated fibres in catch-state induced at ionic strength 0.28 and pH 7.0, relaxe after addition of 5-HT to the low Ca2+ solution. No relaxing effect is observed when the catch-state is induced at ionic strength 0.06 and pH 6.5.

  4. 4.

    Cyclic AMP (c-AMP) induces the same relaxing effects as 5-HT on chemically treated fibres in the same conditions of ionic strength, pH and Ca2+ concentration.

  5. 5.

    The results are in disagreement with the hypothesis that the catch-state in ABRM is due to a lowering of the intracellular ionic strength and pH; they suggest that the intracellular physiological conditions are rather near ionic strength 0.28 and pH 7.0 even in catch-state. We suppose that the relaxing effect of 5-HT, is not be due to a decrease of the intracellular free Ca2+ level but rather to an increase of the rate of Ca2+ release from the contractile proteins, and that it is mediated through c-AMP and intracellular relaxing mediator.

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References

  1. Baguet, F.: The catch-state in glycerol extracted fibres from a Lamellibranch smooth muscle (ABRM) Pflügers Arch.340, 19–34 (1973)

  2. Baguet, F., Marchand-Dumont, G.: The muscular membrane and calcium activation of the contractile system of a Lamellibranch smooth muscle (ABRM). Pflügers Arch.354, 75–85 (1975)

  3. Bloomquist, E., Curtis, B. A.: The action of serotonin on calcium-45 efflux from the anterior byssal retractor muscle of Mytilus edulis. J. gen. Physiol.59, 476–485 (1972)

  4. Cole, R. A., Twarog, B. M.: Relaxation of catch in a molluscan smooth muscle. I. Effects of drugs which act on the adenyl cyclase system. Comp. Biochem. Physiol.43A, 321–330 (1972)

  5. Dölling, B., Achazi, R. K., Zebe, E., Ahlert, V.: 3′,5′-Adenosinmonophosphat im hyssus-Retraktor-muskel der Miesmuschel Mytilus edulis. Naturwissenschaften59, 313 (1972)

  6. Johnson, W. H., Kahn, S. J., Szent-Györgyi, A. G.: Paramyosin and contraction of “catch muscles”. Science130, 160–161 (1959)

  7. Julian, F. J.: The effect of calcium on the force-velocity relation of briefly glycerinated muscle fibres. J. Physiol. (Lond.)218, 117–146 (1971)

  8. Nauss, J. M., Davies, R. E.: Changes in inorganic phosphate and arginine during the development, maintainance and loss of tension in the anterior byssus retractor muscle of Mytilus edulis. Biochem. Z.345, 173–187 (1966)

  9. Stössel, W., Zebe, E.: Zur intracellulären Regulation der Kontraktionsaktivität. Pflügers Arch.302 38–56 (1958)

  10. Twarog, B. M.: Factors influencing contraction and catch in Mytilus smooth muscle. J. Physiol. (Lond.)192, 847–856 (1967)

  11. Twarog, B. M., Muneoka, Y.: Calcium and the control of contraction and relaxation in a molluscan catch-muscle. Cold Spr. Harb. Symp. quant. Biol, 489–503 (1973)

  12. York, B., Twarog, B. M.: Evidence for the release of serotonin by relaxing nerves in molluscan muscle. Comp. Biochem. Physiol.44A, 423–430 (1973)

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Marchand-Dumont, G., Baguet, F. The control mechanism of relaxation in molluscan catch-muscle (ABRM). Pflugers Arch. 354, 87–100 (1975). https://doi.org/10.1007/BF00584505

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Key words

  • 5-Hydroxytryptamine
  • Cyclic AMP
  • Catch-State
  • Smooth Muscle
  • Muscular Membrane