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Measurements of the perivascular PO2 in the vicinity of the pial vessels of the cat

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Abstract

\(P_{O_2 } \) 's in the environment of the pial microvessels of the cat were measured using recessed tip oxygen microelectrodes. Measurements were made on the surface of vessels with internal diameters ranging from 200μm to 22μm. Blood oxygen partial pressures were also measured inside these vessels by penetrating the vessels with sharpened electrodes. Both intravascular and extravascular\(P_{O_2 } \) values decreased progressively from the large arterial vessels down to the small arterioles. The observed values of intravascular\(P_{O_2 } \) showed a systematic longitudinal decrease from 98.5±10.7 (SEM) mm Hg in the largest vessels down to 72.6±3.6 mm Hg in the smallest vessels. In addition to the longitudinal gradient, a transmural gradient was observed across the walls of the microvessels. The difference between blood\(P_{O_2 } \) and vessel surface\(P_{O_2 } \) was 27.0±2.5 mm Hg in the largest vessels and 6.0±2.2 in the smallest. The mean wall thickness in these groups of vesseis were 27.0±1.5 and 7.5±0.8 μm respectively. Measurements of the minimum tissue\(P_{O_2 } \) on the exposed surface of the cortex yielded a value of 25.4±6.6 mm Hg. Systemic arterial partial pressure of oxygen averaged 94.7±4.7 mm Hg. The data indicate that significant gradients for oxygen exist both longitudinally and radially in association with the pial vessels. The longitudinal gradients represent losses of oxygen from the precapillary vessels. The transmural gradients are apparently the result of both consumption by the microvessel wall and diffusional gradients due to oxygen flux into the extravascular space.

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References

  1. 1.

    Baez, S.: Recording of microvascular dimensions with an imagesplitter television microscope. J. Appl. Physiol.211, 299–301 (1966)

  2. 2.

    Betz, E., Wünnenberg, W.: Anpassungsvorgänge der Gehirndurchblutung an Sauerstoffmangel. Arch. Physik. Therapie16, 45–55 (1964)

  3. 3.

    Cook, B. H., Granger, H. J., Taylor, A. E.: Metabolism of coronary arteries and arterioles. A histochemical study. Microvasc. Res.14, 145–159 (1977)

  4. 4.

    Davies, P. W., Bronk, D. W.: Oxygen tension in mammalian brain. Fed. Proc.16, 689–692 (1969)

  5. 5.

    Duling, B. R., Berne, R. M.: Longitudinal gradients in periarteriolar oxygen tension. A possible mechanism for the participation of oxygen in local regulation of blood flow. Circ. Res.27, 669–678 (1970)

  6. 6.

    Grote, J., Kreuscher, R., Schubert, R., Russ, H. J.: New studies on the influence of\(P_a {\text{O}}_2\) and\(P_a {\text{CO}}_2\) on regional and total cerebral blood flow. 6th European Conference on the Microcirculation, pp. 294–297. Basel: Karger 1971

  7. 7.

    Guyton, A. C., Ross, J. M., Carrier, O., Walker, J. R.: Evidence for tissue oxygen demand as the major factor causing autoregulation. Circ. Res.14/15 (Suppl. 1) I60-I68 (1964)

  8. 8.

    Herbert, D. A., Mitchell, R. A.: Blood gas tensions and acid-base balance in awake cats. J. Appl. Physiol.30, 434–437 (1971)

  9. 9.

    Howard, R. O., Richardson, D. W., Smith, M. H., Patterson, J. L.: Oxygen consumption of arterioles and venules as studied in the Cartesian diver. Circ. Res.16, 187–196 (1965)

  10. 10.

    Kety, S. S., Schmidt, C. F.: The effects of altered arterial tensions of carbon dioxide and oxygen on cerebral blood flow and oxygen consumption of normal young men. J. Clin. Invest.27, 484–492 (1948)

  11. 11.

    Kontos, H. A., Wet, E. P., Raper, A. J., Rosenblum, W. I., Navari, R. M., Patterson, J. L.: Role of tissue hypoxia in local regulation of cerebral microcirculation. Am. J. Physiol.234, H582-H591 (1978)

  12. 12.

    Kosan, R. L., Burton, A. C.: Oxygen consumption of arterial smooth muscle as a function of active tone and passive stretch. Circ. Res.18, 79–88 (1966)

  13. 13.

    Lassen, N. A.: Control of cerebral circulation in health and disease. Circ. Res.34, 749–760 (1974)

  14. 14.

    Leniger-Follert, E., Lübbers, D. W., Wrabetz, W.: Regulation of local tissue\(P_{O_2 } \) of the brain cortex at different arterial O2 pressures. Pflügers Arch.359, 81–96 (1975)

  15. 15.

    Lübbers, D. W.: The oxygen pressure field in the brain and its significance for the normal and critical oxygen supply of the brain. In: Oxygen transport in blood and tissue (D. W. Lübbers, U. C. Luft, G. Thews, E. Witzleb, eds.), pp. 67–92. Berlin, Heidelberg, New York: Springer 1968

  16. 16.

    Pittman, R. N., Duling, B. R.: Effects of altered carbon dioxide tension on hemoglobin oxygenation in hamster cheek pouch microvessels. Microvasc. Res.13, 211–224 (1977)

  17. 17.

    Severinghaus, J. W., Lassen, N.: Step hypocapnia to separate arterial from tissue\(P_{{\text{CO}}_2 }\) in the regulation of cerebral blood flow. Circ. Res.20, 272–278 (1967)

  18. 18.

    Silver, I. A.: Some observations on the cerebral cortex with an ultramicro, membrane-covered, oxygen electrode. Med. Electron. Biol. Engn.3, 377–387 (1965)

  19. 19.

    Stosseck, K.: Hydrogen exchange through the pial vessel wall and its meaning for the determination of the local cerebral blood flow. Pflügers Arch.320, 111–119 (1970)

  20. 20.

    Whalen, W. J., Canfield, R., Nair, P.: Effects of breathing O2 or O2+CO2 and of the injection of neurohumors on the\(P_{O_2 } \) of cat cerebral cortex. Stroke1, 194–200 (1970)

  21. 21.

    Whalen, W. J., Riley, J., Nair, P.: A microelectrode for measuring intracellular\(P_{O_2 } \). J. Appl. Physiol.23, 798–801 (1967)

  22. 22.

    Wiederhielm, C. A.: Distensibility characteristics of small blood vessels. Fed. Proc.24, 1075–1084 (1965)

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Supported by grants from: The Von Humboldt Foundation, Federal Republic of Germany; United States Public Health Service No. NHLBI-12792; and the Deutsche Forschungsgemeinschaft

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Duling, B.R., Kuschinsky, W. & Wahl, M. Measurements of the perivascular PO2 in the vicinity of the pial vessels of the cat. Pflügers Arch. 383, 29–34 (1979). https://doi.org/10.1007/BF00584471

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Key words

  • Microcirculation
  • Local control
  • Brain
  • Arterioles
  • Tissue\(P_{O_2 } \)