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  • Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology
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Lactate dehydrogenase and creatine kinase activities in isolated edema fluid and peripheral blood in cold-induced brain edema

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Abstract

A study of the alterations in lactate dehydrogenase and creatine kinase isoenzyme patterns in isolated grey and white matter edema fluid and in peripheral blood is described with a view to possible clinical use in severe brain-injury.

The breakdown of the blood-brain barrier, caused by the infliction of a cold injury to the cerebral cortex of cats, resulted in a shift in the LD isoenzyme distribution in favour of the faster moving fractions and the appearance of brain-type CK in the peripheral blood. Total lactate dehydrogenase activity demonstrated no statistical significant changes during the experiment and total creatine kinase showed only slight increased values in the samples collected simultaneously with the infliction of a cold lesion. This strongly suggest that measurements of these parameters in peripheral blood are of little value as indicators of brain damage.

Alterations in isoenzyme patterns observed in edema fluids are reflected in peripheral blood. The presence, therefore, of brain-type creatine kinase acompanied by a relative high amount of lactate dehydrogenase H-sub unit in peripheral blood, can be considered as adequate indices of severe brain damage.

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References

  1. 1.

    Brightman MW, Klatzo I, Olsson Y, Reese TS (1970) The bloodbrain barrier to proteins under normal and pathological conditions. J Neurol Sci 10:215–239

  2. 2.

    Cao A, Devirgillis S, Lippi C, Trabalza N (1969) Creatine kinase isoenzymes in serum of children with neurological disorders. Clin Chim Acta 23:475–478

  3. 3.

    Dubo H, Park DC, Pennington RJT, Kalbag RM, Walton JN (1967) Serum creatine-kinase in cases of stroke, head injury and meningitis. Lancet 2:743–748

  4. 4.

    Gazendam J, Go KG, van Zanten AK (1979) Composition of isolated edema fluid in cold-induced brain edema. J Neurosurg 51:70–77

  5. 5.

    Gazendam J, Go KG, Van der Meer JJ, Zuiderveen F (1980) Changes of electrical impedance in edematous cat brain during hypoxia and after intracerebral ouabain injection. Exp Neurol (in press)

  6. 6.

    Graune A, Adler S, Fässler H, Rieder HP, Schärer M, Wüthrich R (1976) Liquorprotein-Elektrophoresen bei nicht entzündlichen Erkrankungen des Zentralnervensystems. Schweiz Med Wochenschr 106:1602–1606

  7. 7.

    Johanson GB (1972) Agarose gel electrophoresis. Scand J Clin Lab Invest 29 (Suppl. 124:7–19

  8. 8.

    Kaste M, Somer H, Kontinnen A (1978) Heart type creatine kinase isoenzyme (CK MB) in acute cerebral disorders. Br Heart J 40:802–805

  9. 9.

    Klatzo I, Miquel J, Otenasek R (1962) The application of fluorescein labeled serum proteins (FLSP) to the study of vascular permeability in the brain. Acta Neuropathol (Berl) 2:144–160

  10. 10.

    Lee J, Olszewski J (1959) Permeability of cerebral blood vessels in healing of brain wounds. Neurology 9:7–14

  11. 11.

    Lindblom U, Aberg B (1972) The pattern of S-LDH isoenzymes and S-GOT after traumatic brain injury. Scand J Rehab Med 4:61–72

  12. 12.

    Olsson Y, Hossmann KA (1970) Fine structural localization of exudated protein tracers in the brain. Acta Neuropathol (Berl) 16:103–116

  13. 13.

    Pappius HM, Gulati DR (1963) Water and electrolyte content of cerebral tissue in experimentally induced edema. Acta Neuropathol 2:451–460

  14. 14.

    Rabow L (1977) Lactate dehydrogenase (LD), hydroxybutyrate dehydrogenase (HBD), and LD-isoenzymes in brain tissue. Acta Neurochir 36 (1–2) 61–70

  15. 15.

    Rabow L, Kristensson K (1977) Changes in lactate dehydrogenase isoenzyme patterns in patients with tumours of the central nervous system. Acta Neurochir 36 (1–2) 71–81

  16. 16.

    Rabow L, Tibbling G (1977) Serum activities of hydroxybutyrate dehydrogenase (HBD) and isoenzymes of lactate dehydrogenase (LD) as an index of traumatic brain injury. Acta Neurochir 37 (3–4) 245–261

  17. 17.

    Schliep G, Felgenhauer K (1974) The alpha2-Macroglobulin level in cerebrospinal fluid; a parameter for the condition of the blood CSF barrier. J Neurol 207:171–181

  18. 18.

    Steinwall O, Klatzo I (1965) Double tracer methods in studies on blood-brain barrier dysfunction and brain edema. Acta Neurol Scand 41 Suppl. 13:591–596

  19. 19.

    Van der Veen KJ, Willebrands AF (1966) Isoenzymes of creatine phosphokinase in tissue extracts and in normal and pathological sera. Clin Chim Acta 13:312–316

  20. 20.

    Zweig MH, Van Steirteghem AC (1979) Increased CK-BB concentrations in serum following cardiac surgery. Clin Chim Acta 93:29–33

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Gazendam, J., Kwarts, E. Lactate dehydrogenase and creatine kinase activities in isolated edema fluid and peripheral blood in cold-induced brain edema. Pflügers Arch. 386, 71–76 (1980). https://doi.org/10.1007/BF00584190

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Key words

  • Brain edema
  • Alterations in lactate dehydrogenase and creatine kinase isoenzyme patterns
  • Hypoxia
  • Sodium pump