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Pflügers Archiv

, Volume 388, Issue 2, pp 169–175 | Cite as

The effects of extracellular potassium, ouabain, and prostaglandins on intracellular potassium activity in sheep cardiac Purkinje fibers

  • Michael Wiederholt
  • Peteris Danisevskis
  • Lothar L. Hansen
  • Hans-Jürgen Lichey
  • Klaus D. Platsch
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands

Abstract

(1) Intracellular K activity (a K i ) of sheep heart Purkinje fibers was measured using K-selective microelectrodes (liquid ion exchanger).a K i in the resting state with an extracellular K of 4 mmol·l−1 was 112.9±6.1 mmol·l−1 (n=47) for a membrane potential (VM) of −73.3±0.9 mV.VM deviated from the calculated potassium equilibrium potential (EK=−93 mV). (2) When extracellular K was decreased to 2 mmol·l−1 or increased to 6 and 10 mmol·l−1EK changed from −114 to −84 and −73 mV, with little change ina K i . (3)a K i andVM significantly decreased after administration of 10−6 mol·l−1 ouabain. (4) Prostaglandins (PGI2 10–100 μg·l−1 and PGE2 0.01–1 μg·l−1) decreaseda K i without greatly changingVM. The differences betweenVM andEK became smaller. These effects indicate an increase in K permeability and may explain the antiarrhythmic action of prostaglandins.

Key words

Sheep Purkinje fiber Intracellular potassium activity Extracellular potassium concentration Ouabain Prostaglandins 

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Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Michael Wiederholt
    • 1
  • Peteris Danisevskis
    • 1
  • Lothar L. Hansen
    • 1
  • Hans-Jürgen Lichey
    • 2
  • Klaus D. Platsch
    • 1
  1. 1.Institut für Klinische Physiologie, Klinikum SteglitzFreie Universität BerlinBerlin 45
  2. 2.Klinikum Steglitz (Abteilung für Kardiopneumonologie)Medizinische Klinik und PoliklinikBerlin

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