Pflügers Archiv

, Volume 388, Issue 2, pp 169–175 | Cite as

The effects of extracellular potassium, ouabain, and prostaglandins on intracellular potassium activity in sheep cardiac Purkinje fibers

  • Michael Wiederholt
  • Peteris Danisevskis
  • Lothar L. Hansen
  • Hans-Jürgen Lichey
  • Klaus D. Platsch
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


(1) Intracellular K activity (a K i ) of sheep heart Purkinje fibers was measured using K-selective microelectrodes (liquid ion exchanger).a K i in the resting state with an extracellular K of 4 mmol·l−1 was 112.9±6.1 mmol·l−1 (n=47) for a membrane potential (VM) of −73.3±0.9 mV.VM deviated from the calculated potassium equilibrium potential (EK=−93 mV). (2) When extracellular K was decreased to 2 mmol·l−1 or increased to 6 and 10 mmol·l−1EK changed from −114 to −84 and −73 mV, with little change ina K i . (3)a K i andVM significantly decreased after administration of 10−6 mol·l−1 ouabain. (4) Prostaglandins (PGI2 10–100 μg·l−1 and PGE2 0.01–1 μg·l−1) decreaseda K i without greatly changingVM. The differences betweenVM andEK became smaller. These effects indicate an increase in K permeability and may explain the antiarrhythmic action of prostaglandins.

Key words

Sheep Purkinje fiber Intracellular potassium activity Extracellular potassium concentration Ouabain Prostaglandins 


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  1. Blaustein MP (1974) The interrelationship between sodium and calcium fluxes across cell membranes. Rev Physiol Biochem Pharmacol 70:33–82Google Scholar
  2. Blood BE (1975) The influence of low doses of ouabain and potassium ions on sheep Purkinje fibre contractility. J Physiol 251:69–70PGoogle Scholar
  3. Carmeliet EE (1961) Chloride and potassium permeability in cardiac Purkinje fibers. Arscia, S.A. and Presses Academiques Europeenes, BruxellesGoogle Scholar
  4. Civan MM (1978). Intracellular activities of sodium and potassium. Am J Physiol 234, F261-F269Google Scholar
  5. Cohen I, Daut J, Noble D (1976) The effects of potassium and temperature on the pace-maker current, IK2, in Purkinje fibres. J Physiol 260:55–74Google Scholar
  6. De Deckere EAM, Nugteren DH, Tenfloor F (1977) Prostacyclin is the major prostaglandin release from the isolated, perfused rabbit and rat heart. Nature (London) 268:160–163Google Scholar
  7. Dudel J, Trautwein W (1958) Elektrophysiologische Messungen zur Strophantinwirkung am Herzmuskel. Naunyn-Schmiedeberg's Arch Exp Path Pharmakol 232:393–407Google Scholar
  8. Ellis D (1977) The effects of external cations and ouabain on the intracellular sodium activity of sheep Purkinje fibres. J Physiol 273:211–240Google Scholar
  9. Förster W (1976) Prostaglandins and prostaglandin precursors as endogenous antiarrhythmic principles of the heart. Acta Biol Med Ger 35:1101–1112Google Scholar
  10. Förster W, Mest HJ, Mentz P (1973) The influence of PGF on experimental arrhythmias. Prostaglandins 3:895–904Google Scholar
  11. Fozzard HA, Lee CO (1976) Influence of changes in external potassium and chloride ions on membrane potential and intracellular potassium ion activity in rabbit ventricular muscle. J Physiol 256:663–689Google Scholar
  12. Fries S, Isenberg G (1977) Sheep Purkinje fiber: Intracellular potassium activity measured by ion sensitive electrodes in comparison with potassium reversal potential. Pflügers Arch 368:R3Google Scholar
  13. Godfraind T, Ghysel-Burton J (1977) Binding sites related to ouabain-induced stimulation on inhibition of the sodium pump. Nature 265:165–166Google Scholar
  14. Hansen LL, Koch M, Platsch KD, Wiederholt M (1979) Intracellular potassium activity of isolated human and rabbit corneal epithelium. Exp Eye Res 29:367–378Google Scholar
  15. Hauswirth O, Singh B (1979) Ionic mechanisms in heart muscle in relation to the genesis and the pharmacological control of cardiac arrhythmias. Pharmacol Rev 30:5–63Google Scholar
  16. Kline R, Morad M (1976) Potassium efflux and accumulation in heart muscle. Evidence from K+ electrodes experiments. Biophys J 16:367–372Google Scholar
  17. Lee CO, Fozzard HA (1979) Membrane permeability during low potassium depolarization in sheep cardiac Purkinje fibers. Am J Physiol 237:C156-C165Google Scholar
  18. Mest HJ, Förster W (1978) The antiarrhythmic action of prostacyclin (PGI2) on aconitine enduced arrhythmias in rats. Acta Biol Med Ger 37:827–828Google Scholar
  19. Miura DS, Hoffmann BF, Rosen MR (1977) The effect of extracellular potassium on the intracellular potassium ion activity and transmembrane potentials of beating canine cardiac Purkinje fibers. J Gen Physiol 69:463–474Google Scholar
  20. Müller MD (1965) Ouabain effects on cardiac contraction, action potential, and cellular potassium. Circ Res 17:46–56Google Scholar
  21. Opie LH, Nathan D, Lubbe W (1979) Biochemical aspects of arrhythmogenesis and ventricular fibrillation. Am J Cardiol 43:131–148Google Scholar
  22. Robinson RA, Stokes RH (1970) Electrolyte solutions, 2nd Ed. Butterworths, London, pp 223–253Google Scholar
  23. Schoenberg M, Fozzard HA (1979) The influence of intercellular clefts on the electrical properties of sheep cardiac Purkinje fibers. Biophys J 25:217–234Google Scholar
  24. Schwartz A, Lindenmayer GE, Allen JC (1975) The sodium-potassium adenosine triphosphatase: Pharmacological, physiological and biochemical aspects. Pharmacol Rev 27:3–134Google Scholar
  25. Selden R, Smith TW (1972) Ouabain pharmacokinetics in dog and man: determination by radioimmunoassay. Circulation 45:1176–1182Google Scholar
  26. Sivakoff M, Pure E, Hsueh W, Needleman P (1979) Prostaglandins and the heart. Fed Proc 38:78–82Google Scholar
  27. Walker JL (1971) Ionic specific liquid ion exchanger microelectrode. Anal Chem 43:89A-93AGoogle Scholar
  28. Weidmann S (1956) Elektrophysiologie der Herzmuskelfaser. Huber Verlag, StuttgartGoogle Scholar
  29. Zijlstra WG, Brunsting JR, Ten Hoor F, Vergroesen AJ (1972) Prostaglandin E1 and cardiac arrhythmia. Eur J Pharmacol 18:392–395Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Michael Wiederholt
    • 1
  • Peteris Danisevskis
    • 1
  • Lothar L. Hansen
    • 1
  • Hans-Jürgen Lichey
    • 2
  • Klaus D. Platsch
    • 1
  1. 1.Institut für Klinische Physiologie, Klinikum SteglitzFreie Universität BerlinBerlin 45
  2. 2.Klinikum Steglitz (Abteilung für Kardiopneumonologie)Medizinische Klinik und PoliklinikBerlin

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