Pflügers Archiv

, Volume 388, Issue 2, pp 159–164 | Cite as

Adrenergic control of bicarbonate absorption in the proximal convoluted tubule of the rat kidney

  • Y. L. Chan
Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands


The effects of norepinephrine and phenoxybenzamine on bicarbonate absorption in the rat proximal convoluted tubule were studied by simultaneous microperfusion of tubule and peritubular capillaries. Bicarbonate was determined by using a pH-sensitive membrane electrode system. The rates of bicarbonate absorption\((J_{HCO_3 } )\) were examined in the same proximal tubule before and after the addition of norepinephrine or phenoxybenzamine. When the proximal tubule was perfused with Ringer solution and peritubular capillaries were perfused with albumin Ringer solution,\(J_{HCO_3 }\) was 145±3.3 pEq/min×mm. Addition of 2×10−6 mol/l norepinephrine to the capillary perfusate caused a 21% increase in\(J_{HCO_3 }\). Addition of 2×10−6 mol/l phenoxybenzamine to the capillary perfusate caused a 12% decrease in\(J_{HCO_3 }\). Addition of both norepinephrine and phenoxybenzamine to the capillary perfusate caused a 19% decrease in\(J_{HCO_3 }\). However, there was no significant effect on\(J_{HCO_3 }\) observed when either norepinephrine or phenoxybenzamine was added to the luminal perfusate. These results suggest that adrenergic nerves participate in the regulation of renal tubular bicarbonate absorption through the direct action of norepinephrine on adrenergic receptors located at the basolateral side of the proximal tubule.

Key words

Microperfusion Rat kidney Bicarbonate reabsorption Norepinephrine Alpha adrenergic blocker 


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  1. 1.
    Barajas L, Muller J (1973) The innervation of the juxtaglomerular apparatus and surrounding tubules: A quantitative analysis by serial section electron microscopy. J Ultrastruct Res 43:107–132Google Scholar
  2. 2.
    Bello-Reuss E, Colindres RE, Pastoriza-Munoz E, Mueller RA, Gottschalk CW (1975) Effects of acute unilateral renal denervation in the rat. J Clin Invest 56:208–217Google Scholar
  3. 3.
    Bello-Reuss E, Trevino DL, Gottschalk CW (1976) Effect of renal sympathetic nerve stimulation on proximal water and sodium reabsorption. J Clin Invest 57:1104–1107Google Scholar
  4. 4.
    Benscath P, Bonvalet J-P, de Rouffignac C (1972) Tubular factors in denervation diuresis and natriuresis. In: Wirz H, Spinelli F (eds) Recent advances in renal physiology. International symposium on renal handling of sodium. Karger, Basel, pp 96–106Google Scholar
  5. 5.
    Brasitus TA, Field M, Kimberg DV (1976) Intestinal mucosal cyclic GMO: Regulation and relation to ion transport. Am J Physiol 231:275–282Google Scholar
  6. 6.
    Burg MB, Green N (1977) Bicarbonate transport by isolated perfused rabbit proximal convoluted tubules. Am J Physiol 233:F307-F314Google Scholar
  7. 7.
    Chan YL (1976) Cellular mechanisms of renal tubular transpart ofL-dopa and its derivatives in the rat: Microperfusion studies. J Pharmacol Exp Ther 199:17–24Google Scholar
  8. 8.
    Chan JCM, Grushkin CM, Malekzadek M, Better OS, Fine RN (1973) The adaptation of hydrogen ion excretion associated with nephron reduction in post-transplant patients. Pediatr Res 7:712Google Scholar
  9. 9.
    Gill JR Jr, Casper AGT (1971a) Depression of proximal tubular sodium reabsorption in the dog in response to renal beta-adrenergic stimulation by isoproterenol. J Clin Invest 50:112–118Google Scholar
  10. 10.
    Gill JR Jr, Casper AGT (1971b) Renal effects of adenosine 3′,5′ cyclic monophosphate and dibutyril adenosine 3′,5′ cyclic monophosphate. J Clin Invest 50:1231–1240Google Scholar
  11. 11.
    Gill JR Jr, Tate J, Kelly G (1971) Evidence that guanosine 3′,5′-cyclic monophosphate but not guanosine 5′-monophosphate increase sodium reabsorption by the proximal tubule. Proc Am Soc Nephrol 5:26Google Scholar
  12. 12.
    Gottschalk CW (1979) Renal nerves and sodium excretion. Ann Rev Physiol 41:229–240Google Scholar
  13. 13.
    Kaminsky NI, Ball JH, Broadus AE, Hardman JT, Sutherland EW, Liddle GW (1970) Hormonal effects on extracellular cyclic nucleotides in man. Trans Assoc Am Physicians 83:235–243Google Scholar
  14. 14.
    Karlmark B (1973) The determination of bicarbonate in nanoliter samples. Anal Biochem 53:1–11Google Scholar
  15. 15.
    Karlmark B, Sohtell M, Ulfedahl HR (1971) A pH-glass electrode for nanoliter biological samples. Acta Soc Med Upsal 76:58–62Google Scholar
  16. 16.
    Kurokawa K, Massry SG (1973) Interaction between catecholamines and vasopressin on renal medullary cyclic AMP of rat. Am J Physiol 225:825–829Google Scholar
  17. 17.
    Liang CT, Sacktor B (1977) Preparation of renal cortex basallateral and brush border membranes. Localization of adenylate cyclase and guanylate cyclase activities. Biochim Biophys Acta 466:474–487Google Scholar
  18. 18.
    Lief PD, Mutz BF, Bank N (1979) Effect of cyclic AMP on hydrogen ion secretion by turtle urinary bladder. Kidney Int 16:103–112Google Scholar
  19. 19.
    Lozada E, Soberman R, Gliedman M, Veith F (1969) Hydrogen ion excretion of transplated kidneys. Brill NY Acad Med 45:977Google Scholar
  20. 20.
    Lucci MS, Warnock DG, Rector FC Jr (1979) Carbonic anhydrase-dependent bicarbonate reabsorption in the rat proximal tubule. Am J Physiol 236:F58-F65Google Scholar
  21. 21.
    Murer H, Hoper U, Kinne R (1976) Sodium/proton antiport in brush-border membrane vesicles isolated from rat small intestine and kidney. Biochem J 154:597–604Google Scholar
  22. 22.
    Prosnitz EH, DiBona GF (1978) Effect of decreased renal sympathetic nerve activity on renal tubular sodium reabsorption. Am J Physiol 235:F557-F563Google Scholar
  23. 23.
    Slick GL, Aguilera AJ, Zambrashi EJ, DiBona GF, Kaloyanides GJ (1975) Renal neuradrenergic transmission. Am J Physiol 229:60–65Google Scholar
  24. 24.
    Smith HW (1937) The physiology of the kidney. Oxford University Press, New York, pp 176–181Google Scholar
  25. 25.
    Sonnenberg H, Deetjen P (1964) Methode zur Durchströmung einzelner Nephronabschnitte. Pflügers Arch gesamte Physiol 278:669–674Google Scholar
  26. 26.
    Spitzer A, Windhager EE (1972) Continuous in vivo perfusion of postglomerular and capillary network in superficial rat kidney cortex. Yale J Biol Med 45:307–311Google Scholar
  27. 27.
    Szalay L, Bencsath P, Takacs L (1977) Effect of splanchnicotomy on the renal excretion of inorganic phosphate in the anesthetized dog. Pflügers Arch 367:283–286Google Scholar
  28. 28.
    Szalay L, Bencsath P, Takacs L (1977) Effect of splanchnicotomy on the renal excretion of para-aminohippuric acid in the anesthetized dog. Pflügers Arch 367:287–290Google Scholar
  29. 29.
    Szalay L, Bencsath P, Takacs L (1977) Effect of sphlanchnicotomy on the renal excretion ofD-glucose in the anesthetized dog. Pflügers Arch 369:79–84Google Scholar
  30. 30.
    Ullrich KJ, Frömter E, Baumann K (1969) Micropuncture and microanalysis in kidney physiology. In: Passow H, Stampfli R (eds) Laboratory techniques in membrane biophysics. Springer, Berlin Heidelberg New York, pp 106–129Google Scholar
  31. 31.
    Zinche H, Ott NT, Woods JE, Wilson DM (1976) The role of denervation in renal transplantation of renal function in the dog. Invest Urol 14:210–212Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • Y. L. Chan
    • 1
  1. 1.Department of Physiology and Biophysics, College of MedicineUniversity of Illinois at the Medical CenterChicagoUSA

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