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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Effect of hydrostatic pressure on ADH induced osmotic water flow in toad bladder

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  • 1 Citations

Abstract

The effect of hydrostatic pressure (HP) on antidiuretic hormone (ADH) stimulated osmotic water flow (Jv) across the toad urinary bladder was evaluated. Jv for ADH-stimulated bladders was significantly reduced by an elevation of the serosal HP gradient to 1 cm H2O. Subsequent elimination of the HP gradient resulted in a recovery of Jv. Serosal HP also caused a reversible increase in sucrose permeability (P sucrose). For ADH-treated bladders fixed with glutaraldehyde during serosal HP exposure, subsequent exposure to a mucosal or serosal HP gradient caused acceleration or inhibition of Jv, respectively. The reduction in ADH-associated Jv with serosal HP was apparently caused by a back-flux of water through a paracellular pathway. Jv and P sucrose were not affected by mucosal HP during ADH stimulation. The results suggest a specific sensitivity of a paracellular pathway to a small serosal HP gradient in bladders with ADH-stimulated water flow. The reversibility of this effect on P sucrose suggests that the elements comprising the apical junctions are dynamic structures capable of recovering at least some of their permeability properties.

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References

  1. 1.

    Bentzel CJ, Hainu B, Edelman A, Anagnostopoulos T, Benedetti EL (1976) Effect of plant cytokinens on microfilaments and tight junction permeability. Nature 264:666–668

  2. 2.

    Bracho H, Erlij D, Martinez-Palomo A (1971) The site of the permeability barrier in frog skin epithelim. J Physiol (London) 213:50–62

  3. 3.

    Berry CA, Boulpaep EL (1975) Nonelectrolyte permeability of the paracellular pathway in Necturus proximal tubule. Am J Physiol 228:581–595

  4. 4.

    Cereijido M, Robbins ES, Dolan WJ, Rotunno CA, Sabatini DD (1978) Polarized monolayers formed by epithelial cells on a permeable translucent support. J Cell Biol 77:853–880

  5. 5.

    Civan MM, Di Bona DR (1974) Pathways for movement of ions and water across toad urinary bladder. J Membr Biol 19:195–220

  6. 6.

    Claude P, Goodenough DA (1973) Fracture faces of zonulae occludentes from “tight” and “leaky” epithelia. J Cell Biol 58:390–400

  7. 7.

    Croker BP Jr, Tisher CC (1972) Factors affecting fluid movement and intercellular space formation in the toad bladder. Kidney Int 1:145–155

  8. 8.

    Di Bona DR, Civan MM, Leaf A (1969) The cellular specificity of the effect of vasopressin on toad urinary bladder. J Membr Biol 1:79–91

  9. 9.

    Di Bona DR, Civan MM (1973) Pathways for movement of ions and water across toad urinary bladder. I. Anatomical site of transepithelial shunt pathways. J Membr Biol 12:101–128

  10. 10.

    Eggena P (1972) Glutaraldehyde — fixation method for determining the permeability to water of the toad urinary bladder. Endocrinol 91:240–246

  11. 11.

    Hakim AA, Lifson N (1969) Effect of pressure on water and solute transport by dog intestinal mucosa in vitro. Am J Physiol 216:276–284

  12. 12.

    Hays RM, Leaf A (1962) Studies on the movement of water through the isolated toad bladder and its modification by vasopressin. J Gen Physiol 45:905–919

  13. 13.

    Levine SD, Kachadorian WA (1981) Barriers to water flow in vasopressin treated toad urinary bladder. J Membr Biol 61:(in press)

  14. 14.

    Machen TE, Erlij D, Wooding FBP (1972) Permeable junctional complexes. The movement of lanthanum across rabbit gallbladder and intestine. J Cell Biol 54:302–312

  15. 15.

    Onstad GR, Schoenfield LJ, Higgins JA (1967) Fluid transfer in the everted human gallbladder. J Clin Invest 46:606–614

  16. 16.

    Peachy LD, Rasmussen H (1961) Structure of toad urinary bladder as related to its physiology. J Biophys Biochem Cytol 10:529–549

  17. 17.

    Schermann J, Agerup B, Persson E (1974) Correlation between luminal hydrostatic pressure and proximal tubular fluid reabsorption in the rat kidney. Pflügers Arch 350:145–165

  18. 18.

    Snedecor GW, Cochran WG (1967) Statistical methods (6th edn). Chapter 4. Iowa State University Press, Ames Iowa, p 91

  19. 19.

    Strum JM (1977) Lanthanum “staining” of the lateral and basal membrane of the mitochondria-rich cell in toad bladder epithelium. J Ultrastruct Res 59:126–139

  20. 20.

    Tisher CG, Yarger WR (1973) Lanthanum permeability of the tight junction (zonula occludens) in the renal tubule of the rat. Kidney Int 3:238–250

  21. 21.

    van Os, Wiedner CH, Wright EM (1979) Volume flows across gallbladder epithelium induced by small hydrostatic and osmotic gradients. J Membr Biol 49:1–20

  22. 22.

    Voute CL, Mollgard K, Ussing HH (1975) Quantitative relationship between active sodium transport, expansion of endoplasmic reticulum and specialized vacuoles (“Scalloped Sacs”) in the outermost living cell layer of the frog skin epithelium (Rana temporaria). J Membr Biol 21:273–289

  23. 23.

    Wade JB, DiScala VA (1971) The effect of osmotic flow on the distribution of horseradish peroxidase within the intercllular spaces of toad bladder epithelium. J Cell Biol 51:553–558

  24. 24.

    Wade JB, Revel J-P, DiScala VA (1973) Effect of osmotic gradients on intercellular junctions of the toad bladder. Am J Physiol 224: 407–415

  25. 25.

    Whittembury G, Sugino N, Solomon AK (1960) Effect of antidiuretic hormone and calcium on the equivalent pore radius of kidney slices from Necturus. Nature 187:699–701

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Correspondence to Bernard Rosenbaum.

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Rosenbaum, B., Lombardo, G. & DiScala, V.A. Effect of hydrostatic pressure on ADH induced osmotic water flow in toad bladder. Pflugers Arch. 393, 243–247 (1982). https://doi.org/10.1007/BF00584077

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Key words

  • Hydrostatic pressure
  • Toad bladder function
  • Vasopressin