Pflügers Archiv

, Volume 356, Issue 3, pp 277–286 | Cite as

Renin measurement in blood collected from the efferent arteriole of the kidney of the rat

  • C. I. Johnston
  • P. G. Matthews
  • J. M. Davis
  • T. Morgan
Short Communications and Technical Notes
Received:

Summary

Plasma renin could be detected in 16 nl of rat plasma by incubating at 50°C, pH 6.2 with 20 μl of a rat renin substrate. In 60 nl samples it could be estimated and differences in concentration of 20% could be detected.

Plasma renin concentration was measured in blood from the superficial efferent arterioles of a rat's kidney. The concentrations of renin in different efferent arterioles of the same rat were similar. The renin concentration of efferent arteriolar blood was 361±189 (S.D.) ng Al ml−1 (blood) hour−1 which was not different from the renin concentration of simultaneously taken femoral artery blood (340±217).

Plasma renin concentration can be measured in efferent arteriolar blood and will allow the control of renin secretion to be studied.

Key words

Renin Efferent Arteriole Feedback Control Macula densa Angiotensin 
This is a preview of subscription content, log in to check access.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Assaykeen, T. A., Ganong, W. F.: The sympathetic nervous system and renin secretion.In: Frontiers in neuroendocrinology, W. F. Ganong, and L. Martin, eds. New York: Oxford University Press 1971Google Scholar
  2. 2.
    Blaine, E. H., Davis, J. O.: Evidence for a renal vascular mechanism in renin release: new observations with graded stimulation by aortic constriction. Circulat. Res.28, 11, 118 (1971)Google Scholar
  3. 3.
    Dahlheim, H., Granger, P., Thurau, K.: A sensitive method for determination of renin activity in the single juxtaglomerular apparatus of the rat kidney. Pflügers Arch.321, 303 (1970)Google Scholar
  4. 4.
    Guyton, A. C., Lanston, J. B., Navar, G.: Theory for renal autoregulation by feedback at the juxtaglomerular apparatus. Circulat. Res.15, 187 (1964)Google Scholar
  5. 5.
    Hartoft, P. M., Sutherland, L. M., Hartroft, W. S.: Juxtaglomerular cells as the source of renin: Further studies with the fluorescent antibody technique and the effect of passive transfer of antirenin. Canad. med. Ass. J.90, 163 (1964)Google Scholar
  6. 6.
    Herbert, V., Lau, V. S., Gottlieb, G. W., Bleicher, S. J.: Coated charcoal immunoassay of insulin. J. clin. Endocr.25, 1375 (1965)Google Scholar
  7. 7.
    Hierholzer, K., Butz, M., Mueller-Suur, R., Lichtenstein, I.: Pressure measurements in proximal surface tubules of the rat: Single nephron filtration rate and tubuloglomerular feedback. Yale J. Biol. Med.45, 224 (1972)Google Scholar
  8. 8.
    Johnston, C. I., Mendelsohn, F., Casley, D.: Evaluation of renin and angiotensin assays and their clinical application. Med. J. Aust.1, 126 (1971)Google Scholar
  9. 9.
    Johnston, C. I., Mendelsohn, F. A. O., Doyle, A. E.: Metabolism of angiotensin II in sodium depletion and hypertension in humans. Circulat. Res.30, 203 (1972)Google Scholar
  10. 10.
    Leyssae, P. P.: Dependence of glomerular filtration rate on proximal tubular reabsorption of salt. Acta physiol. Scand.56, 236 (1963)Google Scholar
  11. 11.
    Leyssac, P. O.: The in vivo effect of angiotensin on the proximal tubular reabsorption of salt in rat kidneys. Acta physiol. Scand.62, 436 (1964)Google Scholar
  12. 12.
    Mogil, R., Itskovitz, H. D., Russel, J. H., Murphy, J. J.: Renal innervation and renin activity in salt metabolism and hypertension. Amer. J. Physiol.216, 693 (1969)Google Scholar
  13. 13.
    Nairn, R. C., Fraser, K. B., Chadwick, C. S.: The histological localisation of renin with fluorescent antibody. Brit. J. exp. Path.40, 155 (1959)Google Scholar
  14. 14.
    Navar, L. G., Burke, T. J., Robinson, R. R., Clapp, J. R.: Distal tubular feedback in the autoregulation of single nephron glomerular filtration rate. J. clin. Invest.53, 516 (1974)Google Scholar
  15. 15.
    Schnermann, J., Persson, A. E. G., Agerup, B.: Tubuloglomerular feedback. Nonlinear relation between glomerular hydrostatic pressure and loop of Henle perfusion rate. J. clin. Invest.52, 862 (1973)Google Scholar
  16. 16.
    Schnermann, J., Wright, F. S., Davis, J. M., v. Stackelberg, W., Grill, G.: Regulation of superficial nephron filtration rate by tubuloglomerular feedback. Pflügers Arch.318, 147 (1970)Google Scholar
  17. 17.
    Skinner, S. L.: Improved assay methods for renin “concentration” and “activity” in human plasma. Methods using selective denaturation of renin substrate. Circulat. Res.20, 391 (1967)Google Scholar
  18. 18.
    Skinner, S. L., McCubbin, J. W., Page, I. H.: Renal baroreceptor control of renin release. Science141, 814 (1963)Google Scholar
  19. 19.
    Thurau, K.: Renal haemodynamics. Amer. J. Med.36, 698 (1964)Google Scholar
  20. 20.
    Thurau, K.: Micropuncture evaluation of local control of arteriolar resistance of kidney and brain. Circulat. Res.29, 106 (1971)Google Scholar
  21. 21.
    Thurau, K., Schnermann, J., Nagel, W., Horster, M., Wahl, M.: Composition of tubular fluid in the macula densa segment as a factor regulating the juxtaglomerular apparatus. Circulat. Res.20/21, 11, 79 (1967)Google Scholar
  22. 22.
    Tobian, L., Tomboulian, A., Janecek, J.: Effect of high perfusion pressure on the granulation of juxtaglomerular cells in an isolated kidney. J. clin. Invest.38, 605 (1959)Google Scholar
  23. 23.
    Vander, A. J.: Control of renin release. Physiol. Rev.47, 359–382 (1967)Google Scholar

Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • C. I. Johnston
    • 1
    • 2
  • P. G. Matthews
    • 1
    • 2
  • J. M. Davis
    • 1
    • 2
  • T. Morgan
    • 1
    • 2
  1. 1.Department of MedicineAustin HospitalHeidelbergAustralia
  2. 2.Repatriation HospitalHeidelbergAustralia

Personalised recommendations