Antonie van Leeuwenhoek

, Volume 59, Issue 4, pp 263–268 | Cite as

Pyrimidine base and ribonucleoside utilization by thePseudomonas alcaligenes group

  • Thomas P. West


Pyrimidine base and ribonucleoside utilization was investigated in the two type strains of thePseudomonas alcaligenes group. As sole sources of nitrogen, the pyrimidine bases uracil, thymine and cytosine as well as the dihydropyrimidine bases dihydrouracil and dihydrothymine supported the growth ofPseudomonas pseudoalcaligenes ATCC 17440 but neither these bases nor pyrimidine nucleosides supportedPseudomonas alcaligenes ATCC 14909 growth. Ribose, deoxyribose, pyrimidine and dihydropyrimidine bases as well as pyrimidine nucleosides failed to be utilized by eitherP. pseudoalcaligenes orP. alcaligenes as sole carbon sources. The activities of the pyrimidine salvage enzymes nucleoside hydrolase, cytosine deaminase, dihydropyrimidine dehydrogenase and dihydropyrimidinase were detected in cell-free extracts ofP. pseudoalcaligenes andP. alcaligenes. InP. pseudoalcaligenes, the levels of cytosine deaminase, dihydropyrimidine dehydrogenase and dihydropyrimidinase could be affected by the nitrogen source present in the culture medium.

Key words

cytosine deaminase dihydropyrimidinase dihydropyrimidine dehydrogenase nucleoside hydrolase Pseudomonas pyrimidines 


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Copyright information

© Kluwer Academic Publishers 1991

Authors and Affiliations

  • Thomas P. West
    • 1
  1. 1.Department of ChemistrySouth Dakota State UniversityBrookingsUSA

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