Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope
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The design of a small, inexpensive temperature controlled bath (0.25 ml volume) for electrophysiological studies of isolated cells is described. The design creates a uniform temperature (±1° C) from ambient temperature to approximately 37° C over a bath chamber area of 0.6×1 cm for flow rates from zero to 2ml/min. Access for microelectrodes and unobstructed preparation viewing with an inverted microscope is afforded by a finely stranded heater sandwiched between two glass cover slips and located bencath the bath chamber. Battery operation of the feedback temperature controller and solution pre-heater minimizes electrical interference.
Key wordsPerfusion chamber Isolated heart cell Electrophysiology Temperature regulation Bath design
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