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Pflügers Archiv

, Volume 403, Issue 3, pp 318–323 | Cite as

Versatile temperature controlled tissue bath for studies of isolated cells using an inverted microscope

  • N. B. Datyner
  • G. A. Gintant
  • I. S. Cohen
Excitable Tissues and Central Nervous Physiology Instruments and Techniques

Abstract

The design of a small, inexpensive temperature controlled bath (0.25 ml volume) for electrophysiological studies of isolated cells is described. The design creates a uniform temperature (±1° C) from ambient temperature to approximately 37° C over a bath chamber area of 0.6×1 cm for flow rates from zero to 2ml/min. Access for microelectrodes and unobstructed preparation viewing with an inverted microscope is afforded by a finely stranded heater sandwiched between two glass cover slips and located bencath the bath chamber. Battery operation of the feedback temperature controller and solution pre-heater minimizes electrical interference.

Key words

Perfusion chamber Isolated heart cell Electrophysiology Temperature regulation Bath design 

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References

  1. Cohen IS, Datyner NB, Gintant GA (1984) A study of potassium currents in acutely dissociated canine cardiac Purkinje myocytes J Physiol 349:49Google Scholar
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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • N. B. Datyner
    • 1
  • G. A. Gintant
    • 1
  • I. S. Cohen
    • 1
  1. 1.Department of Physiology and BiophysicsState University of New York at Stony BrookStony BrookUSA

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