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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Effects of the antisecretory factor in pigs

Abstract

The effect of the antisecretory factor (ASF) on experimental porcine enterotoxin-induced jejunal secretion was tested. The heat-labile enterotoxin (LT) fromEscherichia coli and cholera toxin (CT) was used for challenge in ligated intestinal loops. Less than 10 units of ASF inhibited the LT-induced secretion, while that due to CT required more than 10 units of ASF. ASF was effective only when administered prior to toxin challenge, and could be given either intravenously or intra-intestinally. Mixing of ASF with specific anti-ASF antibodies prior to injection abolished its antisecretory effect. LT- and CT-induced secreted fluid contained equal concentrations of Na+, K+ and Cl, and the ionic concentration was not affected by ASF. Less than 0.1 units of ASF per pituitary gland was present in 3- and 5-week old pigs, while it increased to 4.5 units in 28-week old animals, and to 12.2 units in pigs older than two years. However, after intra-intestinal vaccination with 2.0 mg CT, the pituitary ASF content in the 5-week old animals increased to 2.0 units within 24h.

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Abbreviations

ASF:

antisecretory factor

CT:

cholera toxin

LT:

the heat-labile enterotoxin fromEscherichia coli

References

  1. 1.

    Argenzio RA (1980) Glucose-stimulated fluid absorption in the pig small intestine during the early stage of swine dysentery. Am J Vet Res 41:2000–2006

  2. 2.

    Carpenter CCJ (1972) Cholera and other enterotoxin-related diarrheal diseases. J Infect Dis 126:551–564

  3. 3.

    Cooke HJ (1986) Neurobiology of the intestinal mucosa. Gastroenterology 90:1057–1081

  4. 4.

    Craig IP (1972) The enterotoxic enteropathies. In: Smith H, Pearce JH (eds) Microbial pathogenicity in man and animals. Cambridge University Press, Cambridge. pp 129–155

  5. 5.

    Field M (1976) Regulation of active ion transport in the small intestine. In: Elliot K, Knight I (eds) Acute diarrhea in childhood. Ciba Foundation, Symposium 42. Elsevier, Amsterdam, pp 109–122

  6. 6.

    Hirst TR, Sanchez I, Kapu IB, Hardy JSJ, Holmgren J (1984) Mechanism of toxin secretion byVibrio cholerae investigated in ofEscherichia coli. Proc Natl Acad Sci USA 81:7752–7756

  7. 7.

    Kimberg DV (1974) Cyclic nucleotides and their role in gastrointestinal secretion. Gastroenterology 67:1023–1064

  8. 8.

    Lange S (1982) A rat model for anin vivo assay of enterotoxic diarrhea. Microbiol Lett 15:239–242

  9. 9.

    Lange S, Lönnroth I (1984) Passive transfer of protection against cholera toxin in the rat intestine. Microbiol Lett 24:165–168

  10. 10.

    Lange S, Lönnroth I (1984) Bile and milk from cholera toxin treated rats contain a hormone-like factor which inhibits diarrhea induced by the toxin. Int Arch Allergy Appl Immunol 79:270–275

  11. 11.

    Lange S, Lönnroth I, Palm A, Hydén H (1985) An inhibitory protein of intestinal fluid secretion reverses neuronal GABA transport. Biochem Biophys Res Commun 130:1032–1036

  12. 12.

    Lönnroth I, Lange S (1984) Purification and characterization of a hormone-like factor which inhibits cholera secretion. FEBS Lett 177:104–108

  13. 13.

    Lönnroth I, Lange S (1985) A hormone-like protein from the pituitary gland inhibits intestinal hypersecretion induced by cholera toxin. Regul Pept (Suppl 4) 216–218

  14. 14.

    Lönnroth I, Lange S (1986) Purification and characterization of the antisecretory factor — a protein in the central nervous system and in the gut which inhibits intestinal hypersecretion induced by cholera toxin. Biochim Biophys Acta 883:138–144

  15. 15.

    Lönnroth I,Hannson H-A, Lange S (1984) Intestinal adaption to cyclic AMP-mediated hypersecretion induced by heat-labile enterotoxin ofVibrio cholerae andEscherichia coli. Acta Pathol Microbiol Immunol Scand (B) 92:53–60

  16. 16.

    Morishita T, Hibi T, Asakuva H, Tsuchiya M (1977) Clinical studies on cholera-variation of blood hormones and amines in a course of cholera. In: Fukumi H, Zinnaka Y (eds) Proc 12th Joint Conf US-Japan cooperative medical science program cholera panel. National Institute of Health, Tokyo, pp 65–71

  17. 17.

    Sack RB (1975) Human diarrheal disease caused by enterotoxigenicEscherichia coli. Annu Rev Microbiol 29:333–354

  18. 18.

    Skadhauge E (1978) Analysis of computer models. In: Gupta BL, Moreton RB, Oschman SL, Wall BJ (eds) Transport of ions and water in animals. Academic Press, London, pp 145–165

  19. 19.

    Smyth CI, Olsson E, Moncalvo C, Söderlind O, Örskov F, Örskov I (1981) K99 antigen-positive enterotoxigenicEscherichia coli from piglets with diarrhea in Sweden. J Clin Microbiol 13:252–257

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Correspondence to Stefan Lange.

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Lange, S., Lönnroth, I. & Skadhauge, E. Effects of the antisecretory factor in pigs. Pflugers Arch. 409, 328–332 (1987). https://doi.org/10.1007/BF00583485

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Key words

  • Jejunal secretion
  • Pig
  • Enterotoxin
  • Nerve peptide
  • Passive transfer
  • Ion transport
  • Ligated loops