Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Genetic evidence for ribosomal antisuppressors inPodospora anserina

  • 32 Accesses

  • 37 Citations

Summary

Antisuppressors were screened for with the help of informational suppressors inPodospora anserina. Four mutations in the AS1 locus and two in the AS2 locus were isolated, using allele non specific suppressors supposed to be ribosomal ambiguity mutations. Four mutations in the AS3 locus and 45 in the AS4 locus were obtained, using a nonsense (tRNA like) suppressor. All antisuppressors are partially dominant. Most mutations in the AS4 locus are lethal. The four mutants at the AS3 locus and 6 out of the 8 viable mutants at the AS4 locus are cold sensitive. Phenotypic properties and action spectra of the antisuppressors suggest that they are restrictive ribosomal mutations.

This is a preview of subscription content, log in to check access.

References

  1. Bayliss, F.T., Vinopal, R.T.: Selection of ribosomal mutants by antibiotic suppression in yeast. Science174, 1339–1341 (1971)

  2. Biswas, D.K., Gorini, L.: Restriction, De-restriction and mistranslation in missense suppression. Ribosomal discrimination of transfer RNA's. J. molec. Biol.64, 119–134 (1972)

  3. Cooper, D., Banthorpe D.V., Wilkie, D.: Modified ribosomes conferring resistance to cycloheximide in mutants ofS. cerevisiae. J. molec. Biol.26, 347–350 (1967)

  4. Elseviers, D., Gorini, L.: Direct selection of mutants restricting efficiency of suppression and misreading levels inE. coli B. Molec. gen. Genet.137, 277–287 (1975)

  5. Esser, K.: An introduction toPodospora anserina. Neurospora Newsletter15, 27–31 (1969)

  6. Gale, E.F., Gundliffe, E., Reynolds, P.E., Richmond, N.H., Waring M.J.: Cycloheximide and related glutarimide antibiotics. In the molecular basis of antibiotic action, pp. 357–361. London: John Wiley and Sons 1972

  7. Garvin, R.T., Gorini, L.: A new gene for ribosomal restriction inEscherichia coli. Molec. gen. Genet.137, 73–78 (1975)

  8. Gilmore, R.A.: Suppressors inSaccharomyces cerevisiae. Genetics56, 641–658 (1967)

  9. Gilmore, R.A., Stewart, J.W., Sherman, F.: Amino acid replacements resulting from supersuppression of nonsense mutants of iso-1-cytochromec from yeast. J. molec. Biol.61, 157–173 (1971)

  10. Gorini, L.: Streptomycin and Misreading of the genetic code. Ribosomes. Cold Spring Harbor Laboratory, pp. 791–803 (1974)

  11. Gorini, L., Jacoby, G., Breckenridge, L.: Ribosomal ambiguity. Cold Spr. Harb. Symp. quant. Biol.31, 657–664 (1966)

  12. Gorman, J.A., Gorman, J.: Genetic anylsis of a gene required for the expression of allele-specific missense suppression inSaccharomyces cerevisiae. Genetics67, 337–352 (1971)

  13. Guthrie, C., Nashimoto, H., Nomura, M.: Structure and function ofE. coli ribosomes. VII. Cold sensitive mutants defective in ribosome assembly. Proc. nat. Acad. Sci. (Wash.)63, 384–391 (1969)

  14. Hawthorne, D.C.: The selection of nonsense suppressors in yeast. Mutations Res.7, 187–197 (1969)

  15. Inge-Vechtomov, S.G.: Antisuppression. Genetika9, 176–178 (1967)

  16. McCready, S.J., Cox, B.S.: Antisuppressors in yeast. Molec. gen. Genet.124, 305–320 (1973)

  17. Ozaki, M., Mizushima, S., Nomura, M.: Identification and functional characterization of the protein controlled by the streptomycin-resistant locus inE. coli. Nature (Lond.)222, 333–339 (1969)

  18. Perrot, M.: Etude des ribosomes chez le champignonPodospora anserina: caractérisation chez une souche sauvage et divers types de souches mutantes. Thèse de 3è cycle, Université de Bordeaux II, France (1975)

  19. Picard, M.: Genetic evidence for a polycistronic unit of transcription in the complex locus “14” inPodospora anserina. I. Genetic and complementation maps. Molec. gen. Genet.111, 35–50 (1971)

  20. Picard, M.: Genetic evidence for a polycistronic unit of transcription in the complex locus “14” inPodospora anserina. II. Genetic analysis of informational suppressors. Genet. Res. (Camb.)21, 1–15 (1973a)

  21. Picard, M.: Mise en évidence d'une unité de transcription polygénique et de suppresseurs informationnels chez l'ascomycètePodospora anserina: analyse génétique. Thèse, Université de Paris XI, Centre d'Orsay, France (1973b)

  22. Rizet, G., Engelmann, C.: Contribution à l'étude génétique d'un ascomycète tétra-sporé:Podospora anserina. Rev. Cytol. Biol. Veget.2, 202–304 (1949)

  23. Roberds, D.R., DeBusk, A.G.: Cold-sensitive mutants ofNeurospora crassa. J. Bact.115, 1121–1129 (1973)

  24. Rosset, R., Gorini, L.: A ribosomal ambiguity mutation. J. molec. Biol.39, 95–112 (1969)

  25. Rothstein, R.J.: The effect of ochre suppression on meiosis and ascospore formation inSaccharomyces. Ph. D. Thesis, Chicago University, Illinois, USA (1975)

  26. Schindler, D., Grant, P., Davies, J.: Trichodermin resistance, a mutation affecting eucaryotic ribosomes. Nature (Lond.)248, 535–536 (1974)

  27. Seale, T.W.: Supersuppressors inNeurospora crassa. I. Induction, genetic localization and relationship to a missense suppressor. Genetics70, 385–396 (1972)

  28. Simonet, J.M., Zickler, D.: Mutations affecting meiosis inPodospora anserina. I. Cytological studies. Chromosoma (Berl.)37, 327–351 (1972)

  29. Singh, A., Manney, T.R.: Genetic analysis of mutations affecting growth ofSaccharomyces cerevisiae at low temperature. Genetics77, 651–659 (1974)

  30. Stafford, M.E., McLaughlin, C.S.: Trichodermin, a possible inhibitor of the termination process of protein synthesis. J. cell Physiol.82, 121–128 (1973)

  31. Strigini, P., Gorini, L.: Ribosomal mutation affecting efficiency of amber suppression. J. molec. Biol.47, 517–530 (1970)

  32. Tai, P.C., Kessler, D.P., Ingraham, J.L.: Cold sensitive mutations inSalmonella typhimurium which affect ribosome synthesis. J. Bact.97, 1298–1304 (1969)

  33. Terry, K.: Suppression of aNeurospora td mutant that lacks cross-reacting material. Genetics54, 105–114 (1966)

  34. Waldron, C., Roberts, C.F.: Cold sensitive mutants inAspergillus nidulans. II. Mutations a affecting ribosome production. Molec. gen. Genet.134, 115–132 (1974)

  35. Yourno, J., Suskind, S.R.: Suppressor gene action in the tryptophan synthetase system ofNeurospora crassa. I. Genetic studies. II. Biochemical studies. Genetics50, 803–828 (1964)

  36. Zimmerman, R.A., Garvin, R.T., Gorini, L.: Alteration of a 30S ribosomal protein accompanying theram mutation inEscherichia coli. Proc. nat. Acad. Sci. (Wash.)68, 2263–2267 (1971)

Download references

Author information

Additional information

Communicated by F. Gros

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Picard-Bennoun, M. Genetic evidence for ribosomal antisuppressors inPodospora anserina . Molec. Gen. Genet. 147, 299–306 (1976). https://doi.org/10.1007/BF00582881

Download citation

Keywords

  • Action Spectrum
  • Genetic Evidence
  • Phenotypic Property
  • Viable Mutant
  • Specific Suppressor