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Chromosoma

, Volume 101, Issue 7, pp 448–455 | Cite as

The genome of the Mediterranean fruitflyceratitis capitata: Localization of molecular markers by in situ hybridization to salivary gland polytene chromosomes

  • A. Zacharopoulou
  • M. Frisardi
  • C. Savakis
  • A. S. Robinson
  • P. Tolias
  • M. Konsolaki
  • K. Komitopoulou
  • F. C. Kafatos
Original Articles

Abstract

We hybridized cloned DNA segments to salivary gland polytene chromosomes of the medfly,Ceratitis capitata, and thus established molecular markers for 24 sites on 6 out of 10 autosomal arms. An additional marker identified a medfly repetitive element that hybridizes to approximately 100 autosomal sites as well as a granular network that is thought to represent theX chromosome. Some of the markers correspond to 9 characterized transcription units, while 17 remain anonymous; at least 3 of the latter are restriction fragment length polymorphism (RFLP) markers. The characterized transcription units document that chromosomal arm 5L ofC. capitata is homologous to theDrosophila melanogaster X chromosome, in agreement with previous inferences based on the extensive conservation of linkage groups in Diptera.

Keywords

Molecular Marker Salivary Gland Linkage Group Developmental Biology Restriction Fragment Length Polymorphism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • A. Zacharopoulou
    • 1
  • M. Frisardi
    • 2
  • C. Savakis
    • 3
    • 4
  • A. S. Robinson
    • 3
  • P. Tolias
    • 2
  • M. Konsolaki
    • 3
  • K. Komitopoulou
    • 5
  • F. C. Kafatos
    • 2
    • 3
  1. 1.Department of Biology, Division of Genetics, Cell and Developmental BiologyUniversity of PatrasGR-PatrasGreece
  2. 2.Department of Cellular and Development BiologyHarvard UniversityCambridgeUSA
  3. 3.Institute of Molecular Biology and Biotechnology-FORTHHeraklion, Crete, Greece
  4. 4.Department of Medical SciencesUniversity of Crete Medical SchoolGR-Heraklion, Crete, Greece
  5. 5.Department of Biochemistry, Cell and Molecular Biology and GeneticsUniversity of AthensAthensGreece

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