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  • Transport Processes, Metabolism and Endocrinology; Kidney, Gastrointestinal Tract, and Exocrine Glands
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Potassium microclimate at the mucosal surface of the proximal and the distal colon of guinea pig

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Abstract

K+ concentrations were measured with K+ sensitive liquid ion exchanger microelectrodes in situ and in vitro in the mucus layer at the luminal cell surface of the proximal and the distal colon in guinea pig.

In a first series of experiments K+ concentrations were increased in the luminal solution from 0 to 70 mmol·l−1; the serosal K+ concentrations were kept in vitro at 5.4 mmol·l−1. In the proximal colon mean K+ concentration in the microclimate was in vitro 7.9±3.5 mmol·l−1, and independent from mucosal concentrations. In the distal colon in vitro, and in situ in the proximal as well as in the distal colon, K+ concentrations in the microclimate were increased slightly when K+ concentrations were elevated in the luminal solution up to 70 mmol·l−1.

In a second series of in vitro studies K+ concentrations were also altered in the serosal fluid. In the proximal and in the distal colon K+ concentrations increased linearily with elevated K+ concentrations in the serosal solutions.

A temporarily interrupted mucosal blood flow resulted in a significant increase in the K+ concentration in the microclimate.

A paracellular shunt pathway and a high preepithelial diffusion barrier for K+ would explain the observed K+ concentrations in the microclimate at the luminal cell surface.

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Correspondence to W. v. Engelhardt.

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Engelhardt, W.v., Kück, U. & Krause, M. Potassium microclimate at the mucosal surface of the proximal and the distal colon of guinea pig. Pflugers Arch. 407, 625–631 (1986). https://doi.org/10.1007/BF00582643

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Key words

  • Guinea pig
  • Colon
  • Potassium
  • Microclimate
  • Mucus layer
  • Preepithelial barrier