Pflügers Archiv

, Volume 375, Issue 3, pp 245–249 | Cite as

Capillary supply in soleus and gastrocnemius muscles of man

  • Pep Andersen
  • Andries J. Kroese
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology


4 healthy men had biopsies taken from the soleus and the lateral head of the gastrocnemius muscle for determination of capillary supply, fibre type distribution and fibre area.

In serial transverse sections slow twitch (ST) and fast twitch (FT) fibres were identified histochemically by myofibrillar adenosine triphosphatase stains and capillaries visualized by a periodic acid Schiff stain after digestion of glycogen by α-amylase.

In soleus, ST and FT fibre type areas were approximately two times greater than in gastrocnemius. FT fibres (11060 μm2) were 50% greater than ST fibres (7520 μm2) in soleus, whereas no difference between FT and ST fibres was observed in gastrocnemius (4730 μm2 and 4310 μm2, respectively). Both muscles were mixed with respect to fibre types but in all subjects soleus had a higher relative content of ST fibres than gastrocnemius. The area occupied by ST fibres relative to total area was 64% in soleus and 50% in gastrocnemius.

Capillary density was 288 capillaries·mm−2 in soleus and 365 in gastrocnemius. Evaluated on the basis of mean number of capillaries in contact with fibres of each type relative to fibre type area (μm−2·10−3) individual ST fibres had a richer capillary supply than FT fibres in both soleus (0.84 and 0.57, respectively) and gastrocnemius (1.12 and 0.92, respectively).

Differences in organization of the capillary network between soleus and gastrocnemius were observed. Capillaries are running mainly parallel to the muscle fibres in gastrocnemius, but tortuously and/or frequently branching in soleus. Tortuously and/or frequently branching capillaries implies nutritional advantages, which will not be reflected in the quantitative data obtained from counts of capillaries made on transverse sectioned muscle. Therefore the absolute values given for soleus and gastrocnemius, respectively, cannot be directly compared.

Key words

Human muscles Soleus Gastrocnemius Capillary density Capillary arrangement Fibre type area 


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  1. Andersen, P.: Capillary density in skeletal muscle of man. Acta Physiol. Scand.95, 203–205 (1975)Google Scholar
  2. Andersen, P., Henriksson, J.: Capillary supply of the quadriceps femoris muscle of man: Adaptive response to exercise. J. Physiol.270, 677–690 (1977)Google Scholar
  3. Ariano, M. A., Armstrong, R. B., Edgerton, V. R.: Hindlimb muscle fiber populations of five mammals. J. Histochem. Cytochem.21, 51–55 (1973)Google Scholar
  4. Barnard, R. J., Edgerton, V. R., Furukawa, T., Peter, J. B.: Histochemical, biochemical, and contractile properties of red, white, and intermediate fibers. Am. J. Physiol.220, 410–414 (1971)Google Scholar
  5. Bergström, J.: Muscle electrolytes in man. Scand. J. Clin. Lab. Invest. Suppl.68, (1962)Google Scholar
  6. Brooke, M. H., Kaiser, K. K.: Some comments on the histochemical characterization of muscle adenosine triphosphatase. J. Histochem. Cytochem.17, 431–432 (1969)Google Scholar
  7. Buchthal, F., Schmalbruch, H.: Contraction times and fibre types in intact human muscle. Acta Physiol. Scand.79, 435–452 (1970)Google Scholar
  8. Costill, D. L., Daniels, J., Evans, W., Fink, W., Krahenbuhl, G., Saltin, B.: Skeletal muscle enzymes and fibre composition in male and female track athletes. J. Appl. Physiol.40, 149–154 (1976)Google Scholar
  9. Edgerton, V. R., Smith, J. L., Simpson, R.: Muscle fibre type populations of human leg muscles. Histochem. J.7, 259–266 (1975)Google Scholar
  10. Edgerton, V. R., Barnard, R. J., Peter, J. B., Maier, A., Simpson, D. R.: Properties of immobilized hind-limb muscles of the Galago senegalensis. Exp. Neurol.46, 115–131 (1975)Google Scholar
  11. Edström, L., Nyström, B.: Histochemical types and sizes of fibers in normal human muscles. Acta Neurol. Scand.45, 257–269 (1969)Google Scholar
  12. Edström, L., Torlegård, K.: Area estimation of transversely sectioned muscle fibers. Z. Wiss. Mikrosk.69, 166–178 (1968/1969)Google Scholar
  13. Folkow, B., Halicka, H. D.: A comparison between “red” and “white” muscle with respect to blood supply, capillary surface area and oxygen uptake during rest and exercise. Microvasc. Res.1, 1–14 (1968)Google Scholar
  14. Gollnick, P. D., Armstrong, R. B., Saubert, C. W., IV, Piehl, K., Saltin, B.: Enzyme activity and fiber composition in skeletal muscle of untrained and trained men. J. Appl. Physiol.33, 312–319 (1972)Google Scholar
  15. Golnick, P. D., Sjödin, B., Karlsson, J., Jansson, E., Saltin, B.: Human soleus muscle: A comparison of fiber composition and enzyme activities with other leg muscles. Pflügers Arch.348, 247–255 (1974)Google Scholar
  16. Gomori, G.: The distribution of phosphatase in normal organs and tissues. J. Cell. Comp. Physiol.17, 71–83 (1941)Google Scholar
  17. Guth, L., Samaha, F. J.: Qualitative differences between actomyosin ATPase of slow and fast mammalian muscle. Exp. Neurol.25, 138–152 (1969)Google Scholar
  18. Hudlická, O., Pette, D., Staudte, H.: The relation between blood flow and enzymatic activities in slow and fast muscles during development. Pflügers Arch.343, 341–356 (1973)Google Scholar
  19. Johnson, M. A., Polgar, J., Weightman, D., Appleton, D.: Data on the distribution of fibre types in thirty-six human muscles. J. Neurol. Sci.18, 111–129 (1973)Google Scholar
  20. Lee, J. C.: Vascular patterns in the red and white muscles of the rabbit. Anat. Rec.132, 597–611 (1958)Google Scholar
  21. Novikoff, A. B., Shin, W., Drucker, J.: Mitochondrial localization of oxidation enzymes: Staining results with two tetrazolium salts. J. Biophys. Biochem. Cytol.9, 47–61 (1961)Google Scholar
  22. Padykula, H. A., Herman, E.: The specificity of the histochemical method for adenosine triphosphatase. J. Histochem. Cytochem.3, 170–195 (1955)Google Scholar
  23. Peter, J. B., Barnard, R. J., Edgerton, V. R., Gillespie, C. A., Stempel, K. E.: Metabolic profiles of three fiber types of skeletal muscle in guinea pigs and rabbits. Biochemistry11, 2627–2633 (1972)Google Scholar
  24. Plyley, M. J., Groom, A. C.: Geometrical distribution of capillaries in mammalian striated muscle. Am. J. Physiol.228, 1376–1383 (1975)Google Scholar
  25. Polgar, J., Johnson, M. A., Weightman D., Appleton, D.: Data on fibre size in thirty-six human muscles. An autopsy study. J. Neurol. Sci.19, 307–318 (1973)Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Pep Andersen
    • 1
  • Andries J. Kroese
    • 1
  1. 1.The August Krogh InstituteUniversity of CopenhagenCopenhagenDenmark

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